Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TOLA_RS04975 Genome accession   NC_012691
Coordinates   1048499..1049605 (+) Length   368 a.a.
NCBI ID   WP_012729194.1    Uniprot ID   C4LCN1
Organism   Tolumonas auensis DSM 9187     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1043499..1054605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOLA_RS04945 (Tola_0961) rdgB 1044352..1044942 (-) 591 WP_012729188.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  TOLA_RS04950 (Tola_0962) yggU 1045036..1045326 (-) 291 WP_012729189.1 DUF167 family protein YggU -
  TOLA_RS04955 (Tola_0963) - 1045326..1045877 (-) 552 WP_012729190.1 YggT family protein -
  TOLA_RS04960 (Tola_0964) proC 1045886..1046710 (-) 825 WP_012729191.1 pyrroline-5-carboxylate reductase -
  TOLA_RS04965 (Tola_0965) - 1046716..1047417 (-) 702 WP_012729192.1 YggS family pyridoxal phosphate-dependent enzyme -
  TOLA_RS04970 (Tola_0966) pilT 1047451..1048488 (+) 1038 WP_012729193.1 type IV pilus twitching motility protein PilT Machinery gene
  TOLA_RS04975 (Tola_0967) pilU 1048499..1049605 (+) 1107 WP_012729194.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TOLA_RS04980 (Tola_0968) yaaA 1049680..1050462 (+) 783 WP_012729195.1 peroxide stress protein YaaA -
  TOLA_RS04985 (Tola_0969) srmB 1050525..1051841 (-) 1317 WP_012729196.1 ATP-dependent RNA helicase SrmB -
  TOLA_RS04990 (Tola_0970) - 1052004..1052660 (+) 657 WP_218916118.1 methyltransferase -
  TOLA_RS04995 (Tola_0971) fldB 1052668..1053186 (-) 519 WP_012729198.1 flavodoxin FldB -
  TOLA_RS05000 (Tola_0972) xerD 1053262..1054158 (+) 897 WP_041609704.1 site-specific tyrosine recombinase XerD -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41020.09 Da        Isoelectric Point: 5.4642

>NTDB_id=34137 TOLA_RS04975 WP_012729194.1 1048499..1049605(+) (pilU) [Tolumonas auensis DSM 9187]
MELANLLRILVDEKGSDLFISVGIQPSLKVNGKIRRLGDQLLEEDEVLNLVRQTMTDERFRSYVENREANFAINCPGLGR
FRVSAFWQQDCPGCSIRRIETVIPTCDELFLPMSVKELAMAKRGLILFVGATGAGKSTTQAAMIGYRNSHANDHILTIED
PVEFVHKHDRSLITQREVGSDTLSFDEGLKSALRQAPDVILIGEIRSEETMEFALSFAETGHLCMATLHANNANQAIERI
LHLVPAEKHRLLMYDLAFNLKAIVAQQLVPTIDGKSRRAAFEIMLNSPLISDIMRKGEVHRLKETMSRSRELGMSTFDQS
LFELYQQGAIGFDEALAFADSSNEVRVMIKMAAGGNFDSGMLDNVTVA

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=34137 TOLA_RS04975 WP_012729194.1 1048499..1049605(+) (pilU) [Tolumonas auensis DSM 9187]
ATGGAACTGGCTAATTTATTGCGCATTTTGGTTGATGAAAAAGGGTCGGATTTATTTATTTCTGTCGGTATCCAGCCTTC
ACTTAAAGTGAACGGTAAGATCCGTCGTCTGGGCGATCAATTGCTGGAGGAAGATGAAGTTCTTAATCTGGTTCGCCAGA
CGATGACGGATGAGCGTTTTCGTTCATATGTGGAAAACAGAGAAGCGAACTTTGCGATCAATTGTCCCGGATTAGGCCGG
TTCCGTGTCAGTGCTTTCTGGCAGCAGGATTGCCCGGGGTGCTCTATCCGGCGTATCGAGACGGTGATCCCAACCTGTGA
TGAGCTGTTTTTGCCAATGTCGGTTAAAGAACTGGCAATGGCAAAGCGCGGGTTGATCCTGTTTGTTGGTGCGACCGGTG
CGGGTAAATCGACGACGCAGGCGGCAATGATTGGTTACCGTAACAGTCATGCGAACGACCATATTCTGACGATTGAAGAC
CCGGTCGAATTCGTGCATAAGCATGATCGCAGTCTGATCACGCAGCGTGAAGTCGGCTCGGATACGCTCTCGTTTGATGA
AGGTCTGAAAAGTGCGTTACGCCAGGCTCCGGACGTTATTCTGATCGGTGAAATTCGTTCTGAAGAGACCATGGAATTTG
CCTTATCGTTTGCGGAAACCGGTCATCTTTGCATGGCTACACTGCATGCGAATAATGCAAACCAGGCTATAGAACGTATT
CTTCACTTGGTGCCGGCAGAAAAGCATCGTCTGCTGATGTACGATCTGGCATTCAACTTAAAAGCTATTGTGGCGCAGCA
ACTGGTGCCGACGATCGATGGTAAAAGCCGGCGGGCTGCATTTGAGATTATGCTTAATTCACCGCTGATCAGTGACATTA
TGCGTAAAGGTGAAGTGCATCGTCTGAAAGAGACAATGTCCCGTTCCCGCGAACTGGGCATGTCTACGTTTGATCAATCT
TTGTTTGAGTTGTATCAGCAAGGTGCGATTGGCTTTGATGAAGCGCTGGCGTTTGCAGATTCAAGCAATGAAGTCCGCGT
CATGATCAAGATGGCTGCTGGTGGTAATTTTGACTCTGGCATGCTGGATAACGTGACCGTCGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4LCN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

55.858

99.728

0.557
  pilU Pseudomonas stutzeri DSM 10701

57.224

95.924

0.549
  pilU Acinetobacter baylyi ADP1

54.902

97.011

0.533
  pilT Acinetobacter baylyi ADP1

42.522

92.663

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.563

96.467

0.391
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.667

91.304

0.38
  pilT Vibrio cholerae strain A1552

41.667

91.304

0.38
  pilT Legionella pneumophila strain Lp02

41.692

89.946

0.375
  pilT Legionella pneumophila strain ERS1305867

41.692

89.946

0.375
  pilT Neisseria gonorrhoeae MS11

40.058

92.935

0.372
  pilT Neisseria meningitidis 8013

40.058

92.935

0.372
  pilT Acinetobacter nosocomialis M2

41.212

89.674

0.37
  pilT Acinetobacter baumannii D1279779

41.212

89.674

0.37
  pilT Acinetobacter baumannii strain A118

41.212

89.674

0.37
  pilT Pseudomonas aeruginosa PAK

40

91.033

0.364

Multiple sequence alignment