Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EKL02_RS16845 Genome accession   NZ_CP035311
Coordinates   3778579..3779622 (+) Length   347 a.a.
NCBI ID   WP_128903112.1    Uniprot ID   A0A410UWG5
Organism   Janthinobacterium sp. 17J80-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3773579..3784622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKL02_RS16815 (EKL02_16815) - 3773690..3774016 (-) 327 WP_128903106.1 hypothetical protein -
  EKL02_RS16820 (EKL02_16820) - 3774126..3774542 (-) 417 WP_128903107.1 DUF4279 domain-containing protein -
  EKL02_RS16830 (EKL02_16830) - 3775871..3776842 (+) 972 Protein_3328 integron integrase -
  EKL02_RS16835 (EKL02_16835) proC 3776893..3777717 (-) 825 WP_128903110.1 pyrroline-5-carboxylate reductase -
  EKL02_RS16840 (EKL02_16840) - 3777765..3778472 (-) 708 WP_128903111.1 YggS family pyridoxal phosphate-dependent enzyme -
  EKL02_RS16845 (EKL02_16845) pilT 3778579..3779622 (+) 1044 WP_128903112.1 type IV pilus twitching motility protein PilT Machinery gene
  EKL02_RS16850 (EKL02_16850) pilU 3779653..3780789 (+) 1137 WP_128903113.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EKL02_RS16855 (EKL02_16855) - 3780795..3781280 (+) 486 WP_128903114.1 glutathione peroxidase -
  EKL02_RS16860 (EKL02_16860) - 3781308..3782033 (-) 726 WP_128903115.1 LrgB family protein -
  EKL02_RS16865 (EKL02_16865) - 3782030..3782362 (-) 333 WP_128903116.1 CidA/LrgA family protein -
  EKL02_RS16870 (EKL02_16870) - 3782427..3782978 (+) 552 WP_128903117.1 cob(I)yrinic acid a,c-diamide adenosyltransferase -
  EKL02_RS16875 (EKL02_16875) folE2 3782988..3783794 (-) 807 WP_128903118.1 GTP cyclohydrolase FolE2 -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37800.43 Da        Isoelectric Point: 6.9395

>NTDB_id=339273 EKL02_RS16845 WP_128903112.1 3778579..3779622(+) (pilT) [Janthinobacterium sp. 17J80-10]
MDISELLAFSVKNNASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMVYDIMNDGQRKVYEEKLEIDFSFAIPGLAR
FRVNAFNQDRGAAAVLRTIPSKILSLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAGMVNHVNETEYAHILTIED
PIEFVHDSKKCLINQREVGPHTHSFTAALRSALREDPDVILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGDEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDGN
LTDLVRRNIISPAAARSAAKTPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=339273 EKL02_RS16845 WP_128903112.1 3778579..3779622(+) (pilT) [Janthinobacterium sp. 17J80-10]
ATGGACATTTCCGAACTCCTCGCTTTTTCCGTCAAGAACAACGCCTCCGACTTGCACCTGTCGGCCGGCTTGCCGCCCAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGGATGTGCACGGCATGGTCTATGACA
TCATGAACGACGGCCAGCGCAAGGTGTATGAAGAAAAGCTGGAAATCGACTTTTCCTTTGCCATTCCGGGCCTGGCGCGC
TTCCGTGTGAATGCCTTCAACCAGGACCGCGGCGCCGCAGCCGTGCTGCGCACCATTCCTTCCAAGATCCTGAGCCTGGA
GCAGTTGAATGCGCCGAAGATCTTTGGCGACCTGGCCCTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACAGGCT
CGGGCAAGTCCACGACCCTGGCCGGCATGGTCAACCATGTCAATGAAACCGAATACGCCCACATCCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGATTCCAAGAAATGCCTGATCAACCAGCGTGAAGTGGGGCCGCATACGCATTCCTTCACCGC
CGCCTTGCGTTCGGCGCTGCGTGAAGATCCGGACGTGATCCTGGTGGGCGAATTGCGCGACCTCGAAACCATCCGCCTGG
CGCTGTCCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGATGAAAAGGAAATGGTCCGCGCCATGCTGTCGGAGTCGCTCCAGGCCGTCATTTCGCAAAC
GCTCCTGAAAACCAAGGATGGTTCGGGTCGCGTCGCCGCCCATGAAATCATGATCGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAAGCCAAGATCGCGCAGATGTATTCAGCCATCCAGACCGGCTCCAACGTCGGCATGCAGACGCTGGACGGCAAC
CTGACGGACCTCGTGCGCCGCAATATCATTTCTCCGGCAGCCGCACGCTCCGCAGCGAAGACCCCGGAAAATTTCCCAGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A410UWG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas aeruginosa PAK

75.072

99.424

0.746
  pilT Pseudomonas stutzeri DSM 10701

73.623

99.424

0.732
  pilT Acinetobacter baylyi ADP1

73.623

99.424

0.732
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.118

0.674
  pilT Vibrio cholerae strain A1552

69.436

97.118

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.284

93.372

0.395
  pilU Vibrio cholerae strain A1552

38.841

99.424

0.386

Multiple sequence alignment