Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EKO29_RS13580 Genome accession   NZ_CP034660
Coordinates   3144294..3145337 (-) Length   347 a.a.
NCBI ID   WP_126669381.1    Uniprot ID   A0A3S9Q7M6
Organism   Colwellia sp. Arc7-635     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3139294..3150337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKO29_RS13560 (EKO29_13560) - 3139384..3140316 (+) 933 WP_126669377.1 AEC family transporter -
  EKO29_RS13565 (EKO29_13565) - 3140343..3140888 (-) 546 WP_126669378.1 DsrE family protein -
  EKO29_RS13570 (EKO29_13570) - 3140933..3142873 (-) 1941 WP_126669379.1 GGDEF domain-containing phosphodiesterase -
  EKO29_RS13575 (EKO29_13575) pilU 3143141..3144268 (-) 1128 WP_126669380.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EKO29_RS13580 (EKO29_13580) pilT 3144294..3145337 (-) 1044 WP_126669381.1 type IV pilus twitching motility protein PilT Machinery gene
  EKO29_RS13585 (EKO29_13585) - 3145408..3146082 (+) 675 WP_126669382.1 YggS family pyridoxal phosphate-dependent enzyme -
  EKO29_RS13590 (EKO29_13590) proC 3146318..3147133 (+) 816 WP_126669383.1 pyrroline-5-carboxylate reductase -
  EKO29_RS13595 (EKO29_13595) - 3147318..3147866 (+) 549 WP_126669384.1 YggT family protein -
  EKO29_RS13600 (EKO29_13600) - 3148034..3148474 (+) 441 WP_126669385.1 DUF4426 domain-containing protein -
  EKO29_RS13605 (EKO29_13605) - 3148630..3149526 (-) 897 WP_126669386.1 EamA family transporter -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38613.19 Da        Isoelectric Point: 6.2334

>NTDB_id=332204 EKO29_RS13580 WP_126669381.1 3144294..3145337(-) (pilT) [Colwellia sp. Arc7-635]
MDITELLAFSVENNASDLHLSAGTPPSIRVDGDVRKLNIPAFDEKDVNALVYDIMNDRQRKEYEEKLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDDLGCPDIFRDISALPRGLVLVTGPTGSGKSTTLAAMVDHINKHKHDHILTIED
PIEFVHENHSCLINQREVHRDTLSFSAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLVKKVGGGRVAAHEIMMGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLVNRGLITKQDAMEKAQDKNQFKGY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=332204 EKO29_RS13580 WP_126669381.1 3144294..3145337(-) (pilT) [Colwellia sp. Arc7-635]
ATGGATATTACCGAATTACTAGCATTTAGTGTGGAAAATAATGCTTCAGATTTACATTTATCAGCCGGAACTCCCCCATC
TATCCGCGTCGATGGTGATGTACGAAAATTAAATATACCAGCATTTGACGAAAAAGATGTGAACGCTTTGGTCTATGATA
TTATGAATGACCGCCAACGCAAAGAGTATGAAGAGAAATTAGAGGTCGATTTTTCTTTTGAAGTACCAAACTTAGCCCGT
TTCAGGGTCAATGCTTTCAATCAAAACCGCGGTCCTGCTGCCGTATTTCGTACCATTCCAAGTAAAATATTGTCATTAGA
TGACTTAGGTTGCCCTGATATTTTTAGAGATATATCGGCATTACCACGCGGACTTGTTTTAGTGACTGGGCCAACAGGTT
CGGGTAAATCTACTACGTTAGCGGCCATGGTTGATCATATTAATAAACATAAGCATGACCATATATTAACGATTGAAGAC
CCCATTGAATTTGTTCATGAAAACCATTCTTGTTTGATTAACCAACGTGAAGTTCACCGCGACACTTTAAGCTTTAGCGC
GGCATTACGCTCAGCTTTACGTGAAGACCCCGATGTTATTCTTGTTGGTGAAATGCGTGATTTAGAAACGATACGTTTAG
CTATGACCGCGGCTGAAACCGGGCATTTGGTCTTTGGCACTTTGCATACCACTTCAGCACCCAAAACAATTGACCGTATT
ATTGATGTTTTCCCAGGGGAAGAAAAGGCGATGGTACGTTCGATGTTGTCAGAATCCTTACGAGCGGTAATTTCGCAAAC
ATTGGTTAAAAAGGTTGGCGGTGGTCGTGTTGCTGCCCATGAAATTATGATGGGTGTGCCAGCAATTCGTAACCTCATTC
GAGAAGATAAAATTGCTCAAATGTATTCTGCCATTCAAACCGGTATGTCTCATGGTATGCAAACCATGGATCAATGTTTA
CAAAATCTTGTTAACCGAGGTTTAATCACTAAGCAAGATGCTATGGAAAAAGCGCAAGATAAAAACCAATTTAAGGGCTA
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9Q7M6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.135

0.758
  pilT Acinetobacter nosocomialis M2

76.453

99.135

0.758
  pilT Acinetobacter baumannii D1279779

76.453

99.135

0.758
  pilT Acinetobacter baumannii strain A118

76.453

99.135

0.758
  pilT Acinetobacter baylyi ADP1

76.163

99.135

0.755
  pilT Pseudomonas aeruginosa PAK

75.872

99.135

0.752
  pilT Legionella pneumophila strain ERS1305867

73.547

99.135

0.729
  pilT Legionella pneumophila strain Lp02

73.547

99.135

0.729
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.976

97.695

0.703
  pilT Vibrio cholerae strain A1552

71.976

97.695

0.703
  pilT Neisseria meningitidis 8013

67.449

98.271

0.663
  pilT Neisseria gonorrhoeae MS11

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

99.424

0.533
  pilU Vibrio cholerae strain A1552

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.751

99.712

0.406
  pilU Acinetobacter baylyi ADP1

40.346

100

0.403

Multiple sequence alignment