Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EKO29_RS13575 Genome accession   NZ_CP034660
Coordinates   3143141..3144268 (-) Length   375 a.a.
NCBI ID   WP_126669380.1    Uniprot ID   A0A3S9Q7M7
Organism   Colwellia sp. Arc7-635     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3138141..3149268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKO29_RS13550 (EKO29_13550) - 3138248..3138802 (+) 555 WP_126669375.1 YqgE/AlgH family protein -
  EKO29_RS13555 (EKO29_13555) ruvX 3138802..3139239 (+) 438 WP_126669376.1 Holliday junction resolvase RuvX -
  EKO29_RS13560 (EKO29_13560) - 3139384..3140316 (+) 933 WP_126669377.1 AEC family transporter -
  EKO29_RS13565 (EKO29_13565) - 3140343..3140888 (-) 546 WP_126669378.1 DsrE family protein -
  EKO29_RS13570 (EKO29_13570) - 3140933..3142873 (-) 1941 WP_126669379.1 GGDEF domain-containing phosphodiesterase -
  EKO29_RS13575 (EKO29_13575) pilU 3143141..3144268 (-) 1128 WP_126669380.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EKO29_RS13580 (EKO29_13580) pilT 3144294..3145337 (-) 1044 WP_126669381.1 type IV pilus twitching motility protein PilT Machinery gene
  EKO29_RS13585 (EKO29_13585) - 3145408..3146082 (+) 675 WP_126669382.1 YggS family pyridoxal phosphate-dependent enzyme -
  EKO29_RS13590 (EKO29_13590) proC 3146318..3147133 (+) 816 WP_126669383.1 pyrroline-5-carboxylate reductase -
  EKO29_RS13595 (EKO29_13595) - 3147318..3147866 (+) 549 WP_126669384.1 YggT family protein -
  EKO29_RS13600 (EKO29_13600) - 3148034..3148474 (+) 441 WP_126669385.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41099.02 Da        Isoelectric Point: 5.5458

>NTDB_id=332203 EKO29_RS13575 WP_126669380.1 3143141..3144268(-) (pilU) [Colwellia sp. Arc7-635]
MNFHSLLKVMVQHEASDMFVTAKLAVSAKINGELTAIDEKVLSADEALGLVHDAMSEKQKLEFEKDKECNFAISIDGIGR
FRVSAFWQRDMAGMVVRRIVTEIPQADDLGLPSILKDVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNQNSRGHILTIED
PVEFVHEHAKCMVTQREVGLDTESFDAALKSSLRQAPDVILIGEIRNQETMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPAEQHDKLLFDLALNLRGIIAQQLIPTCDGNGRVAAIEVLLNSPYVAELIKKGDIGSIKEVMEKSTDQGMQTFDQA
LFHLYQQGLITYADALHHADSPNDLRLMIKLRNNDQGGTGSLSGVTIDGLESKNS

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=332203 EKO29_RS13575 WP_126669380.1 3143141..3144268(-) (pilU) [Colwellia sp. Arc7-635]
ATGAATTTTCATAGTTTATTAAAAGTAATGGTGCAGCATGAAGCATCAGATATGTTTGTCACCGCTAAACTAGCGGTCAG
TGCAAAAATTAATGGCGAGCTTACCGCTATTGATGAAAAGGTTTTATCAGCAGATGAAGCATTAGGTTTGGTGCATGATG
CGATGTCTGAAAAACAAAAACTCGAGTTTGAGAAAGACAAAGAATGTAACTTTGCTATTTCAATTGATGGTATTGGCCGG
TTTCGTGTTTCTGCCTTTTGGCAACGAGACATGGCTGGCATGGTGGTTCGTCGAATTGTTACTGAAATTCCACAAGCGGA
TGATCTAGGCCTACCTTCGATATTAAAAGATGTGGTGATGTCCAAGCGTGGTTTGGTCTTGTTTGTGGGCGGCACAGGTA
CGGGTAAATCAACGTCGATGGCTGCATTGATTGGCTATCGTAATCAAAACTCTCGTGGCCATATTTTGACTATCGAAGAT
CCGGTTGAATTTGTTCATGAACATGCCAAATGTATGGTGACACAACGTGAAGTAGGGCTAGATACTGAAAGTTTTGATGC
CGCGCTAAAAAGTTCATTACGTCAAGCACCTGATGTCATACTTATTGGTGAAATACGTAACCAAGAAACCATGGAGCACG
CGTTAAGTTTCGCAGAAACAGGCCATTTATGTATTGCGACCTTGCATGCTAACAATGCTAACCAAGCTATCGATCGTATT
ATGCATTTAGTGCCCGCAGAGCAACATGACAAGTTACTTTTTGATCTAGCACTAAACTTACGCGGTATTATCGCACAGCA
ATTGATTCCTACCTGCGATGGTAACGGCCGTGTAGCGGCTATTGAAGTACTGCTGAACTCGCCTTACGTTGCCGAATTAA
TTAAAAAAGGTGATATTGGTAGTATCAAAGAAGTGATGGAAAAATCAACTGACCAAGGGATGCAAACCTTCGATCAGGCC
TTGTTTCATTTGTATCAACAAGGGCTTATTACTTACGCTGATGCTTTACATCATGCCGATTCACCGAATGATTTACGCTT
AATGATTAAATTACGCAACAATGATCAAGGTGGAACAGGTTCATTATCTGGCGTTACCATTGATGGTTTAGAATCTAAAA
ACTCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9Q7M7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.064

95.2

0.581
  pilU Acinetobacter baylyi ADP1

59.375

93.867

0.557
  pilU Vibrio cholerae strain A1552

56.25

98.133

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.598

96.8

0.403
  pilT Pseudomonas aeruginosa PAK

39.71

92

0.365
  pilT Acinetobacter baylyi ADP1

40.058

91.2

0.365
  pilT Acinetobacter nosocomialis M2

39.766

91.2

0.363
  pilT Acinetobacter baumannii D1279779

39.766

91.2

0.363
  pilT Acinetobacter baumannii strain A118

39.766

91.2

0.363

Multiple sequence alignment