Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PERMA_RS07450 Genome accession   NC_012440
Coordinates   1447027..1448106 (-) Length   359 a.a.
NCBI ID   WP_012675268.1    Uniprot ID   C0QRH7
Organism   Persephonella marina EX-H1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1442027..1453106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PERMA_RS07435 (PERMA_1501) - 1443139..1444110 (+) 972 WP_012675689.1 CCA tRNA nucleotidyltransferase -
  PERMA_RS10915 (PERMA_1502) - 1444617..1444748 (+) 132 WP_012676117.1 hypothetical protein -
  PERMA_RS07440 (PERMA_1503) mnmA 1444749..1445810 (-) 1062 WP_015898855.1 tRNA 2-thiouridine(34) synthase MnmA -
  PERMA_RS07445 (PERMA_1504) - 1445811..1447013 (-) 1203 WP_012676425.1 type II secretion system F family protein -
  PERMA_RS07450 (PERMA_1505) pilT 1447027..1448106 (-) 1080 WP_012675268.1 type IV pilus twitching motility protein PilT Machinery gene
  PERMA_RS07455 (PERMA_1506) - 1448124..1449857 (-) 1734 WP_012676535.1 ATPase, T2SS/T4P/T4SS family -
  PERMA_RS07460 (PERMA_1507) accC 1449959..1451326 (+) 1368 WP_012675692.1 acetyl-CoA carboxylase biotin carboxylase subunit -
  PERMA_RS07465 (PERMA_1508) - 1451323..1451961 (+) 639 WP_012676097.1 MBL fold metallo-hydrolase -
  PERMA_RS10660 (PERMA_1509) - 1451955..1452812 (+) 858 WP_012675804.1 deoxyribonuclease IV -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 40313.75 Da        Isoelectric Point: 7.5498

>NTDB_id=33116 PERMA_RS07450 WP_012675268.1 1447027..1448106(-) (pilT) [Persephonella marina EX-H1]
MERELSLTIDEIARKSLEMDATDIHITAGAPPTLRIDGKITYLSDYPVLYPRDTQKLIYSIMNEKQKRTFEDKNEVDFSI
GIKGIGRFRVNVFRQRGSVAAALRRIPYEIKPMEELGLIPSVKELCRKSMGLVLVTGPTGSGKTTTLASMIDYINANFQY
HIITIEDPIEYLFPHKKSLVAQREVGNDTGGFAIALKYALREDPDVILVGEMRDLETIKAALTAAETGHLVFATLHTNTA
IQTINRIINVFPEHEQDQIRTELSFVLQGVISQRLLPRIGGGRVLIHEVLIPTTGIRNLIRENKIHQIYGLMQSGQVGTG
MQTMNQSIMRAIREGLITPEDGLKISPEPQELERLLKLK

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=33116 PERMA_RS07450 WP_012675268.1 1447027..1448106(-) (pilT) [Persephonella marina EX-H1]
ATGGAAAGAGAACTTTCTTTAACAATTGATGAGATAGCAAGAAAATCACTTGAGATGGATGCAACGGATATACATATTAC
AGCTGGAGCACCTCCAACACTCAGGATAGACGGTAAGATCACATACCTTTCAGACTATCCTGTTCTATACCCGAGGGACA
CACAGAAACTTATATACTCAATAATGAATGAAAAACAGAAGAGAACATTTGAGGACAAAAATGAGGTTGATTTCTCAATA
GGTATAAAGGGTATAGGAAGATTCAGGGTTAACGTTTTCAGACAGAGGGGAAGTGTTGCAGCAGCCTTAAGAAGGATTCC
TTACGAGATAAAACCTATGGAAGAGCTTGGACTTATACCTTCTGTTAAAGAGCTTTGTAGAAAAAGTATGGGACTTGTTC
TTGTTACAGGTCCAACAGGATCTGGTAAAACAACAACACTTGCATCTATGATTGATTATATAAATGCAAACTTCCAGTAC
CACATAATAACCATTGAAGATCCCATTGAGTATCTTTTCCCTCATAAAAAATCACTTGTTGCACAGAGGGAGGTTGGTAA
CGATACAGGTGGTTTCGCTATAGCACTGAAGTATGCATTAAGGGAAGACCCGGATGTTATACTTGTTGGTGAGATGAGGG
ACCTTGAGACTATAAAGGCAGCACTCACAGCAGCAGAAACAGGACACCTTGTTTTTGCAACACTTCACACAAACACAGCT
ATACAGACTATAAACCGTATAATAAACGTATTCCCTGAACATGAACAGGATCAGATAAGAACAGAGCTCAGTTTTGTTCT
TCAGGGGGTTATATCACAGAGATTACTTCCAAGGATAGGTGGCGGAAGGGTTCTCATACATGAGGTTCTCATCCCGACAA
CAGGTATAAGAAACCTTATAAGAGAAAATAAGATACACCAGATCTATGGTCTTATGCAGTCAGGACAGGTTGGAACAGGT
ATGCAGACGATGAACCAGTCTATTATGAGAGCTATAAGGGAGGGACTGATTACACCTGAAGACGGTCTTAAGATATCACC
TGAACCTCAGGAGCTTGAAAGATTACTAAAACTAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C0QRH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.143

97.493

0.499
  pilT Acinetobacter baumannii strain A118

51.156

96.379

0.493
  pilT Acinetobacter baumannii D1279779

51.156

96.379

0.493
  pilT Acinetobacter nosocomialis M2

51.156

96.379

0.493
  pilT Acinetobacter baylyi ADP1

50.867

96.379

0.49
  pilT Legionella pneumophila strain ERS1305867

52.711

92.479

0.487
  pilT Legionella pneumophila strain Lp02

52.711

92.479

0.487
  pilT Pseudomonas aeruginosa PAK

50.578

96.379

0.487
  pilT Pseudomonas stutzeri DSM 10701

49.133

96.379

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.149

93.315

0.468
  pilT Vibrio cholerae strain A1552

50.149

93.315

0.468
  pilT Neisseria gonorrhoeae MS11

50.156

89.415

0.448
  pilT Neisseria meningitidis 8013

50.156

89.415

0.448
  pilU Pseudomonas stutzeri DSM 10701

43.059

98.329

0.423
  pilU Acinetobacter baylyi ADP1

43.695

94.986

0.415
  pilU Vibrio cholerae strain A1552

40.294

94.708

0.382

Multiple sequence alignment