Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EIP93_RS18235 Genome accession   NZ_CP034276
Coordinates   4021752..4022768 (-) Length   338 a.a.
NCBI ID   WP_125233078.1    Uniprot ID   -
Organism   Pectobacterium versatile strain 14A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4016752..4027768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EIP93_RS18220 (EIP93_18265) nsrR 4017078..4017503 (-) 426 WP_109225256.1 nitric oxide-sensing transcriptional repressor NsrR -
  EIP93_RS18225 (EIP93_18270) - 4017847..4019418 (+) 1572 WP_103971948.1 methyl-accepting chemotaxis protein -
  EIP93_RS18230 (EIP93_18275) - 4019951..4021783 (+) 1833 WP_125233077.1 sensor domain-containing diguanylate cyclase -
  EIP93_RS18235 (EIP93_18280) pilT 4021752..4022768 (-) 1017 WP_125233078.1 type IV pilus twitching motility protein PilT Machinery gene
  EIP93_RS18240 (EIP93_18285) - 4022790..4023503 (+) 714 WP_125233079.1 YggS family pyridoxal phosphate-dependent enzyme -
  EIP93_RS18245 (EIP93_18290) proC 4023605..4024426 (+) 822 WP_125233360.1 pyrroline-5-carboxylate reductase -
  EIP93_RS18250 (EIP93_18295) - 4024476..4025030 (+) 555 WP_010295003.1 YggT family protein -
  EIP93_RS18255 (EIP93_18300) yggU 4025027..4025317 (+) 291 WP_103942383.1 DUF167 family protein YggU -
  EIP93_RS18260 (EIP93_18305) - 4025349..4025942 (+) 594 WP_125233080.1 XTP/dITP diphosphatase -
  EIP93_RS18265 (EIP93_18310) hemW 4025935..4027074 (+) 1140 WP_125233081.1 radical SAM family heme chaperone HemW -
  EIP93_RS18270 (EIP93_18315) - 4027308..4027568 (+) 261 WP_072040255.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36787.99 Da        Isoelectric Point: 6.6280

>NTDB_id=330348 EIP93_RS18235 WP_125233078.1 4021752..4022768(-) (pilT) [Pectobacterium versatile strain 14A]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSAQPSLDALHAPPVFSTLLEKPNGLLLITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFSQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQTLLPAAHGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=330348 EIP93_RS18235 WP_125233078.1 4021752..4022768(-) (pilT) [Pectobacterium versatile strain 14A]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGATGGGGCATTAATGCTGCCTGATGGGCAA
CGCCTGCGGGTTAACGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGCGTTATTCCATCCGCTCAGCCTTCATT
GGACGCGCTACACGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGCTCCTGATTACCGGTGCGACGG
GGAGCGGCAAATCCACAACGCTGGCGGCCATGCTTGGGGCGTTAAACGACCGCTGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTTC
GCAGGCATTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTACGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCTGGTGAAGAAAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCGGTGGTGACGCA
AACACTCTTGCCTGCGGCACACGGTGGCCGCATTGCGCTTTATGAAGTACTGACCGCCACCGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

50.151

97.929

0.491
  pilT Pseudomonas aeruginosa PAK

49.849

97.929

0.488
  pilT Acinetobacter baylyi ADP1

49.547

97.929

0.485
  pilT Acinetobacter baumannii strain A118

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

49.245

97.929

0.482
  pilT Acinetobacter baumannii D1279779

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment