Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EHF44_RS08380 Genome accession   NZ_CP033969
Coordinates   1449565..1450608 (+) Length   347 a.a.
NCBI ID   WP_124683318.1    Uniprot ID   -
Organism   Cupriavidus pauculus strain FDAARGOS_614     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1444565..1455608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHF44_RS08350 (EHF44_08350) - 1444863..1445819 (+) 957 WP_124683312.1 LysR substrate-binding domain-containing protein -
  EHF44_RS08355 (EHF44_08355) - 1446047..1446232 (+) 186 WP_124683313.1 hypothetical protein -
  EHF44_RS08360 (EHF44_08360) - 1446317..1446802 (+) 486 WP_124683314.1 Dps family protein -
  EHF44_RS08365 (EHF44_08365) ubiA 1446901..1447785 (+) 885 WP_124683315.1 4-hydroxybenzoate octaprenyltransferase -
  EHF44_RS08370 (EHF44_08370) proC 1447870..1448688 (-) 819 WP_124683316.1 pyrroline-5-carboxylate reductase -
  EHF44_RS08375 (EHF44_08375) - 1448766..1449455 (-) 690 WP_124683317.1 YggS family pyridoxal phosphate-dependent enzyme -
  EHF44_RS08380 (EHF44_08380) pilT 1449565..1450608 (+) 1044 WP_124683318.1 type IV pilus twitching motility protein PilT Machinery gene
  EHF44_RS08385 (EHF44_08385) pilU 1450618..1451757 (+) 1140 WP_124683319.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EHF44_RS08390 (EHF44_08390) - 1451813..1452307 (+) 495 WP_124683320.1 glutathione peroxidase -
  EHF44_RS08395 (EHF44_08395) - 1452342..1453034 (-) 693 WP_124683321.1 YafY family protein -
  EHF44_RS08400 (EHF44_08400) - 1453048..1453461 (-) 414 WP_124683322.1 VOC family protein -
  EHF44_RS08405 (EHF44_08405) - 1453546..1454271 (-) 726 WP_124683323.1 LrgB family protein -
  EHF44_RS08410 (EHF44_08410) - 1454268..1454642 (-) 375 WP_124683324.1 CidA/LrgA family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38387.16 Da        Isoelectric Point: 6.8704

>NTDB_id=327517 EHF44_RS08380 WP_124683318.1 1449565..1450608(+) (pilT) [Cupriavidus pauculus strain FDAARGOS_614]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLDDLHAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHNSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGMINYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=327517 EHF44_RS08380 WP_124683318.1 1449565..1450608(+) (pilT) [Cupriavidus pauculus strain FDAARGOS_614]
ATGGACATCGCACAGCTTCTGGCCTTCGCGGTCAAGAACAAGGCTTCCGATCTTCACCTCTCGGCAGACATGCCGCCGAT
GGTGCGTATTCACGGGGACATGCGCCGCATCAACGTCGCGGCCATGACGCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAATCCTACGAAGAGCGGCTGGAGGTCGATTTCTCTTTCGAGATCGCCGGGCTGTCCCGC
TTCCGGGTCAACGCCTACAACACCCAGCGCGGCGCCGCGGCCGTGTTCCGGACGATTCCCTCCAAGGTACTGACGCTCGA
CGACCTGCACGCGCCGGCCGTGTTTGCCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTGACCGGGCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGGACCACCGCAACGACAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTCCACAACTCCAAGAAAAGCCTGATCAACCAGCGCGAGCTCGGGCCGCACACGCACTCGTTCGCCAA
CGCGCTGCGCTCGGCGCTGCGCGAGGATCCGGACGTGATCCTGGTGGGCGAACTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACGGGCCACCTTGTCTTTGCCACGCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGGGTC
GTGGACGTGTTTCCGCCCGAGGAAAAGGACATGGTCCGCACGATGCTGTCCGAATCGCTGGAAGCGGTCATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCAACGGCCGGACCGCCGCGCACGAGATCATGATCGCCACGCCGGCCATCCGGCACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTACTCGATGATGCAGACGTCCAGCGGCCTGGGCATGCAGACGCTGGACCAGTGC
CTGAGCGACCTGATCAAGCGCGGCATGATCAACTACAACGACGCGCGGGCGATTGCCAAGAATCCTGACGCCTTCATGGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.387

99.712

0.712
  pilT Acinetobacter baumannii D1279779

71.387

99.712

0.712
  pilT Acinetobacter baumannii strain A118

71.387

99.712

0.712
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Pseudomonas aeruginosa PAK

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

66.86

99.135

0.663
  pilT Neisseria meningitidis 8013

66.86

99.135

0.663
  pilT Legionella pneumophila strain Lp02

66.377

99.424

0.66
  pilT Legionella pneumophila strain ERS1305867

66.377

99.424

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

99.424

0.487
  pilU Acinetobacter baylyi ADP1

40.988

99.135

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Vibrio cholerae strain A1552

40

96.542

0.386

Multiple sequence alignment