Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EHF44_RS08385 Genome accession   NZ_CP033969
Coordinates   1450618..1451757 (+) Length   379 a.a.
NCBI ID   WP_124683319.1    Uniprot ID   A0A3G8GYZ3
Organism   Cupriavidus pauculus strain FDAARGOS_614     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1445618..1456757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHF44_RS08355 (EHF44_08355) - 1446047..1446232 (+) 186 WP_124683313.1 hypothetical protein -
  EHF44_RS08360 (EHF44_08360) - 1446317..1446802 (+) 486 WP_124683314.1 Dps family protein -
  EHF44_RS08365 (EHF44_08365) ubiA 1446901..1447785 (+) 885 WP_124683315.1 4-hydroxybenzoate octaprenyltransferase -
  EHF44_RS08370 (EHF44_08370) proC 1447870..1448688 (-) 819 WP_124683316.1 pyrroline-5-carboxylate reductase -
  EHF44_RS08375 (EHF44_08375) - 1448766..1449455 (-) 690 WP_124683317.1 YggS family pyridoxal phosphate-dependent enzyme -
  EHF44_RS08380 (EHF44_08380) pilT 1449565..1450608 (+) 1044 WP_124683318.1 type IV pilus twitching motility protein PilT Machinery gene
  EHF44_RS08385 (EHF44_08385) pilU 1450618..1451757 (+) 1140 WP_124683319.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EHF44_RS08390 (EHF44_08390) - 1451813..1452307 (+) 495 WP_124683320.1 glutathione peroxidase -
  EHF44_RS08395 (EHF44_08395) - 1452342..1453034 (-) 693 WP_124683321.1 YafY family protein -
  EHF44_RS08400 (EHF44_08400) - 1453048..1453461 (-) 414 WP_124683322.1 VOC family protein -
  EHF44_RS08405 (EHF44_08405) - 1453546..1454271 (-) 726 WP_124683323.1 LrgB family protein -
  EHF44_RS08410 (EHF44_08410) - 1454268..1454642 (-) 375 WP_124683324.1 CidA/LrgA family protein -
  EHF44_RS08415 (EHF44_08415) glcF 1454771..1456033 (-) 1263 WP_124683325.1 glycolate oxidase subunit GlcF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42460.68 Da        Isoelectric Point: 6.4435

>NTDB_id=327518 EHF44_RS08385 WP_124683319.1 1450618..1451757(+) (pilU) [Cupriavidus pauculus strain FDAARGOS_614]
MLDRESASKYINDLLELMVNNRGSDLFITSEFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQVKEFDDTRECNFA
ITAPNAGRFRVSAFIQQGRAGMVVRTINTRIPSVEDLDLPPSLHDIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHIALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFELYEADKITYEDALKNADSLNDLRLMIKLHSTRHRDADLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=327518 EHF44_RS08385 WP_124683319.1 1450618..1451757(+) (pilU) [Cupriavidus pauculus strain FDAARGOS_614]
ATGCTAGACCGCGAAAGCGCCTCGAAGTACATCAACGACCTGCTGGAGCTGATGGTGAACAACCGCGGCTCGGACCTGTT
CATCACGTCGGAATTCCCGCCCGCGATCAAGGTGGACGGCAAGATCACGCCGGTGTCGCAGCAGCCGCTGAACCCGACGC
AGGCCCTGGGGCTGGTGCGATCGATCATGAACGAGCGCCAGGTGAAGGAGTTCGACGACACGCGCGAGTGCAACTTCGCC
ATCACGGCGCCCAACGCCGGGCGGTTCCGCGTATCGGCGTTCATCCAGCAGGGCCGGGCCGGCATGGTGGTGCGGACGAT
CAACACGCGCATCCCGTCGGTGGAGGACCTGGACCTGCCGCCGTCGCTGCATGACATCGTCATGTCCAAGCGCGGCCTGG
TGATCGTCACCGGCGCCACGGGGTCGGGCAAGTCGACGTCGCTGGCGGCGATGCTGGACCACCGCAACGCGCACTCGTAC
GGCCACATCATCACGATCGAGGACCCGATCGAGTACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTGGG
CATCGACACCGAGTCGTGGCACATCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACGGGCCACCTGTGCCTGGCCACGCTGCACGCGAACAAC
GCCAACCAGGCCATCGACCGGATCGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGTTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGGCTGCTGCCGCGCGCCGGCCGCAAGGGCCGCGTGCCGGCCGTGGAGATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAATCGCGCGAGCAG
GGCATGATCTCGTTCGACCAGGCGCTGTTCGAGCTGTACGAGGCCGACAAGATCACGTACGAGGACGCGCTCAAGAACGC
CGACTCGCTGAACGACCTGCGCCTGATGATCAAGCTGCACAGCACGCGCCACCGCGACGCCGACCTGGGCGCCGGCACCG
AGCACCTGAACGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G8GYZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

91.821

0.58
  pilU Acinetobacter baylyi ADP1

59.429

92.348

0.549
  pilU Vibrio cholerae strain A1552

54.27

95.778

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

88.918

0.396
  pilT Legionella pneumophila strain Lp02

44.144

87.863

0.388
  pilT Legionella pneumophila strain ERS1305867

44.144

87.863

0.388
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

42.73

88.918

0.38
  pilT Acinetobacter baylyi ADP1

44.41

84.96

0.377
  pilT Acinetobacter nosocomialis M2

44.201

84.169

0.372
  pilT Acinetobacter baumannii D1279779

44.201

84.169

0.372
  pilT Acinetobacter baumannii strain A118

44.201

84.169

0.372
  pilT Neisseria meningitidis 8013

40.882

89.71

0.367
  pilT Vibrio cholerae strain A1552

43.218

83.641

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.641

0.361

Multiple sequence alignment