Detailed information NTG0326020

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: FNE87_RS04545
• Genome accession: NZ_CP041416
• Coordinates: 956888..957868 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055620.1
• Uniprot ID: -
• Organism: Escherichia coli strain STEC711
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 951888..962868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FNE87_RS04515 (FNE87_04675)	yggM	952403..953410 (+)	1008	WP_000745217.1	DUF1202 family protein	-
FNE87_RS04520 (FNE87_04680)	hemW	953565..954701 (-)	1137	WP_161620243.1	radical SAM family heme chaperone HemW	-
FNE87_RS04525 (FNE87_04685)	rdgB	954694..955287 (-)	594	WP_001174745.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
FNE87_RS04530 (FNE87_04690)	yggU	955295..955585 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
FNE87_RS04535 (FNE87_04695)	yggT	955582..956148 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
FNE87_RS04540 (FNE87_04700)	yggS	956166..956870 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
FNE87_RS04545 (FNE87_04705)	pilT	956888..957868 (+)	981	WP_001055620.1	type IV pilus twitching motility protein PilT	Machinery gene
FNE87_RS04550 (FNE87_04710)	ruvX	958044..958460 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
FNE87_RS04555 (FNE87_04715)	yqgE	958460..959023 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
FNE87_RS04560 (FNE87_04720)	gshB	959132..960082 (-)	951	WP_000593273.1	glutathione synthase	-
FNE87_RS04565 (FNE87_04725)	rsmE	960095..960826 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
FNE87_RS04570 (FNE87_04730)	endA	960906..961613 (-)	708	WP_000286500.1	deoxyribonuclease I	-
FNE87_RS04575 (FNE87_04735)	yggI	961708..962205 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=326020 FNE87_RS04545 WP_001055620.1 956888..957868(+) (pilT) [Escherichia coli strain STEC711]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=326020 FNE87_RS04545 WP_001055620.1 956888..957868(+) (pilT) [Escherichia coli strain STEC711]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365