Detailed information NTG0032500

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: DHAF_RS17595
• Genome accession: NC_011830
• Coordinates: 3755255..3756289 (-)
• Length: 344 a.a.
• NCBI ID: WP_015944641.1
• Uniprot ID: -
• Organism: Desulfitobacterium hafniense DCB-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3750255..3761289

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DHAF_RS17570 (Dhaf_3522)	-	3750699..3751202 (-)	504	WP_015944636.1	hypothetical protein	-
DHAF_RS17575 (Dhaf_3523)	-	3751195..3752667 (-)	1473	WP_041271986.1	PilN domain-containing protein	-
DHAF_RS17580 (Dhaf_3524)	-	3752694..3753455 (-)	762	WP_015944638.1	prepilin peptidase	-
DHAF_RS17585 (Dhaf_3525)	-	3753503..3753991 (-)	489	WP_015944639.1	type II secretion system protein	-
DHAF_RS17590 (Dhaf_3526)	-	3754019..3755242 (-)	1224	WP_015944640.1	type II secretion system F family protein	-
DHAF_RS17595 (Dhaf_3527)	pilT	3755255..3756289 (-)	1035	WP_015944641.1	type IV pilus twitching motility protein PilT	Machinery gene
DHAF_RS17600 (Dhaf_3528)	-	3756306..3758066 (-)	1761	WP_015944642.1	GspE/PulE family protein	-
DHAF_RS17605 (Dhaf_3529)	-	3758059..3758469 (-)	411	WP_035213812.1	hypothetical protein	-
DHAF_RS17610 (Dhaf_3530)	-	3758412..3758945 (-)	534	WP_015944643.1	PulJ/GspJ family protein	-
DHAF_RS17615 (Dhaf_3531)	-	3758980..3759363 (-)	384	WP_015944644.1	type IV pilus modification PilV family protein	-
DHAF_RS17620 (Dhaf_3532)	-	3759360..3759698 (-)	339	WP_015944645.1	type II secretion system protein GspG	-
DHAF_RS17625 (Dhaf_3533)	aroB	3759968..3761050 (-)	1083	WP_005810889.1	3-dehydroquinate synthase	-

Sequence

Protein

• Length: 344 a.a.
• Molecular weight: 37953.41 Da
• Isoelectric Point: 6.8389

>NTDB_id=32500 DHAF_RS17595 WP_015944641.1 3755255..3756289(-) (pilT) [Desulfitobacterium hafniense DCB-2]
MTIEQLLKEAAQRRASDIHLTVDSPPVFRIDGSLVRAEEEILTPNVLDEMARTLMDSAQSSKFKESGEIDFSHSIPDVGR
FRVNAFRQRGAAGIAIRLIPFNISTPEQLGLPPVCIEFANLRKGLVLVTGPTGSGKSTTLASMIDYINRTRSEHIVTVED
PIEYMHKHRLSLVNQREVGSDTQSFANALRAALRQDPDVILVGEMRDLETISTAVTAAETGHLVFSTLHTNDATQAVDRM
IDVFPPFQQQQMRVQLAAVLQGVIAQQLLPRKDQQGRVAAQEILVVTPAVRNLIREGKTHQIANTMQTGGKLGMQSMEKA
IQEHVRAGRISNAVAQEMIQNMGH

Nucleotide

• Length: 1035 bp

>NTDB_id=32500 DHAF_RS17595 WP_015944641.1 3755255..3756289(-) (pilT) [Desulfitobacterium hafniense DCB-2]
ATGACTATAGAGCAACTATTAAAAGAAGCAGCTCAGCGACGTGCCTCGGATATTCATCTTACAGTGGACAGTCCTCCAGT
TTTCCGTATTGATGGTTCTTTGGTAAGGGCTGAAGAAGAGATATTAACTCCCAATGTGCTGGATGAAATGGCGAGAACTT
TAATGGACAGTGCTCAGAGTAGCAAGTTTAAAGAGTCTGGAGAAATAGATTTTTCCCACAGCATTCCCGATGTAGGTCGT
TTCCGTGTCAATGCTTTTCGCCAACGAGGAGCTGCGGGAATCGCCATTCGTCTTATTCCCTTTAATATCTCGACACCGGA
GCAGCTTGGTCTTCCCCCTGTCTGCATCGAGTTTGCCAATTTACGCAAAGGGCTGGTTCTTGTGACGGGTCCTACGGGAA
GCGGGAAGTCTACCACCTTAGCCTCAATGATTGATTACATCAATAGGACCAGGTCGGAGCATATCGTTACCGTTGAAGAT
CCCATCGAGTATATGCATAAACATCGCCTAAGCTTAGTTAATCAGCGGGAAGTGGGAAGTGACACCCAATCCTTTGCCAA
TGCTTTGCGAGCAGCATTGCGGCAGGATCCGGATGTAATCCTCGTCGGTGAAATGCGGGATCTTGAGACCATAAGTACGG
CGGTGACGGCTGCAGAGACAGGGCATCTGGTATTTAGTACCCTCCATACCAATGATGCTACTCAGGCAGTGGACAGAATG
ATCGACGTGTTTCCGCCTTTTCAGCAGCAGCAAATGCGGGTCCAGTTGGCAGCGGTTCTTCAAGGAGTTATCGCCCAACA
GCTGCTTCCTCGTAAGGATCAACAGGGAAGAGTTGCAGCCCAGGAAATTCTTGTTGTCACACCGGCAGTAAGAAATCTCA
TCAGAGAAGGAAAGACCCATCAAATTGCTAACACAATGCAAACCGGCGGTAAACTTGGGATGCAAAGTATGGAGAAAGCT
ATTCAGGAACATGTCCGGGCTGGACGCATCTCCAATGCAGTCGCGCAAGAAATGATACAAAATATGGGCCACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (6-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	57.911	91.86	0.532
pilT	Legionella pneumophila strain Lp02	51.462	99.419	0.512
pilT	Legionella pneumophila strain ERS1305867	51.462	99.419	0.512
pilT	Pseudomonas aeruginosa PAK	50.742	97.965	0.497
pilT	Neisseria meningitidis 8013	50	99.419	0.497
pilT	Vibrio cholerae strain A1552	50.294	98.837	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	50.294	98.837	0.497
pilT	Pseudomonas stutzeri DSM 10701	50.445	97.965	0.494
pilT	Neisseria gonorrhoeae MS11	49.415	99.419	0.491
pilT	Acinetobacter baumannii strain A118	49.405	97.674	0.483
pilT	Acinetobacter nosocomialis M2	49.405	97.674	0.483
pilT	Acinetobacter baumannii D1279779	49.405	97.674	0.483
pilT	Acinetobacter baylyi ADP1	49.107	97.674	0.48
pilU	Vibrio cholerae strain A1552	44.713	96.221	0.43
pilU	Pseudomonas stutzeri DSM 10701	43.202	96.221	0.416
pilU	Acinetobacter baylyi ADP1	40.476	97.674	0.395
pilB	Acinetobacter baylyi ADP1	31.078	100	0.36