Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JL719_RS00865 Genome accession   NZ_CP033381
Coordinates   198142..199179 (+) Length   345 a.a.
NCBI ID   WP_026602881.1    Uniprot ID   -
Organism   Methylomonas sp. LW13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 193142..204179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JL719_RS00840 (U737_00885) - 193359..193622 (+) 264 WP_033158303.1 hypothetical protein -
  JL719_RS00845 (U737_00890) - 193734..195683 (-) 1950 WP_033158302.1 transglutaminase TgpA family protein -
  JL719_RS00850 (U737_00895) - 195686..196636 (-) 951 WP_033158301.1 DUF58 domain-containing protein -
  JL719_RS00855 (U737_00900) - 196633..197538 (-) 906 WP_033158300.1 AAA family ATPase -
  JL719_RS00860 (U737_00905) - 197684..198025 (+) 342 WP_033158299.1 TraR/DksA family transcriptional regulator -
  JL719_RS00865 (U737_00910) pilT 198142..199179 (+) 1038 WP_026602881.1 type IV pilus twitching motility protein PilT Machinery gene
  JL719_RS00870 (U737_00915) pilU 199195..200328 (+) 1134 WP_033158298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JL719_RS00875 (U737_00920) mltB 200346..201527 (+) 1182 WP_033158297.1 lytic murein transglycosylase B -
  JL719_RS00880 (U737_00930) - 202028..202582 (-) 555 WP_129871319.1 hypothetical protein -
  JL719_RS00885 (U737_00935) - 202798..203334 (-) 537 WP_033158295.1 REP-associated tyrosine transposase -
  JL719_RS00890 - 203424..203834 (-) 411 WP_165385903.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38228.15 Da        Isoelectric Point: 7.0292

>NTDB_id=323452 JL719_RS00865 WP_026602881.1 198142..199179(+) (pilT) [Methylomonas sp. LW13]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPSLNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELDAPKFFAELCTKPRGLILVTGPTGSGKSTTLAAMVNHINTNDYAHILTVED
PIEFVHESQKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKGAMVKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=323452 JL719_RS00865 WP_026602881.1 198142..199179(+) (pilT) [Methylomonas sp. LW13]
ATGGATATCGCCGAACTCTTGACCTTTTCCGTCAAAAACAAAGCCTCCGACTTGCATCTTTCCGCCGGCCTGCCGCCGAT
GATTCGGGTGGACGGCGACATCCGCCGTATCAATATCCCGTCCTTAAATCACAAGGAAGTCCACGCGCTGATTTACGACA
TCATGAACGACAAGCAGCGCCGCGATTACGAAGAGTTTCTGGAAACCGACTTCTCGTTCGCGCTGCCCGGCGTGGCTCGC
TTCCGGGTCAACGCCTTCAATCAGGATAGAGGCGCCGGCGCGGTATTCCGGACCATTCCGTCCAAGGTGTTAACCCTGGA
AGAATTGGACGCGCCGAAATTTTTCGCGGAATTGTGTACCAAACCGCGCGGCCTGATTCTAGTGACCGGCCCAACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGGTGAATCACATTAATACCAACGATTACGCACACATCCTGACCGTCGAAGAC
CCTATCGAGTTTGTCCACGAAAGCCAGAAATGCCTAATCAACCAGCGCGAAGTACATCGCGATACCTTAGGTTTCAACGA
AGCCTTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGTGAGATGCGGGATTTGGAAACCATCCGCCTGG
CCTTGACCGCCGCGGAAACCGGCCACTTGGTGTTCGGCACCCTGCATACCACCTCGGCCGCGAAAACCATAGACCGGATT
ATCGACGTGTTCCCTGCCGCCGAAAAAGATATGATCCGGGCCATGCTGTCCGAATCCTTGCAGGCGGTTATCTCGCAAAC
GCTATTGAAAAAAGTCGGTGGCGGCCGGATTGCCGCCCACGAAATCATGGTCGGCACCCCTGCCATCCGCAACCTGATCC
GCGAAGCCAAGGTGGCGCAGATGTATTCCGCGATCCAAACCGGCCGTAAAGACGGCATGCAGACGCTGGATCAAAACCTG
AAGGAATTGGTCGACAAAGGCTTGATTACTGCGAAAGGCGCGATGGTCAAAGCCGTCAACAAAGATATGTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Acinetobacter baylyi ADP1

79.651

99.71

0.794
  pilT Pseudomonas aeruginosa PAK

80.769

97.971

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Neisseria meningitidis 8013

66.472

99.42

0.661
  pilT Neisseria gonorrhoeae MS11

66.181

99.42

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Acinetobacter baylyi ADP1

40.346

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Vibrio cholerae strain A1552

40.413

98.261

0.397

Multiple sequence alignment