Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JL719_RS00870 Genome accession   NZ_CP033381
Coordinates   199195..200328 (+) Length   377 a.a.
NCBI ID   WP_033158298.1    Uniprot ID   A0A2N0V4Z9
Organism   Methylomonas sp. LW13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 194195..205328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JL719_RS00850 (U737_00895) - 195686..196636 (-) 951 WP_033158301.1 DUF58 domain-containing protein -
  JL719_RS00855 (U737_00900) - 196633..197538 (-) 906 WP_033158300.1 AAA family ATPase -
  JL719_RS00860 (U737_00905) - 197684..198025 (+) 342 WP_033158299.1 TraR/DksA family transcriptional regulator -
  JL719_RS00865 (U737_00910) pilT 198142..199179 (+) 1038 WP_026602881.1 type IV pilus twitching motility protein PilT Machinery gene
  JL719_RS00870 (U737_00915) pilU 199195..200328 (+) 1134 WP_033158298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JL719_RS00875 (U737_00920) mltB 200346..201527 (+) 1182 WP_033158297.1 lytic murein transglycosylase B -
  JL719_RS00880 (U737_00930) - 202028..202582 (-) 555 WP_129871319.1 hypothetical protein -
  JL719_RS00885 (U737_00935) - 202798..203334 (-) 537 WP_033158295.1 REP-associated tyrosine transposase -
  JL719_RS00890 - 203424..203834 (-) 411 WP_165385903.1 hypothetical protein -
  JL719_RS00895 (U737_00945) - 203812..204708 (-) 897 WP_033158293.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42046.36 Da        Isoelectric Point: 6.6667

>NTDB_id=323453 JL719_RS00870 WP_033158298.1 199195..200328(+) (pilU) [Methylomonas sp. LW13]
MDFKALLALMVEKKASDLFITAGRPPSMKVNGKVVEVSKSVLTSEQTMMLVLSIMSQRQRDEFENTHECQFALSAHSLGR
FRVSAFTQRDSAGMVLRRIETTIPSAEELHLPPVLKELIMYKRGLVMFVGATGTGKSTSLAALIRHRNENSSGHIISIED
PIEFIHPHKGCIITQREVGLDTESFEVALKNTLRQAPDVILIGEVRSRDTMQHAITFAETGHLCVCTLHANNANQALDRI
LHFFPEEMHSQLFMDLSLNLRGIVAQQLIQRADGKGRYPAIEILLNTPLASDMIRKGEVHKLKELMKSSREHGMQTFDQA
LYDLYTAGKIGYDDALHAADSRNEVRLMIKLGAENVNFETAGMTLAENDDEGGSLFK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=323453 JL719_RS00870 WP_033158298.1 199195..200328(+) (pilU) [Methylomonas sp. LW13]
ATGGACTTTAAAGCCCTATTGGCCCTGATGGTGGAAAAGAAAGCGTCCGACTTGTTCATCACCGCCGGCCGGCCGCCGTC
GATGAAAGTCAACGGCAAAGTCGTCGAAGTGTCCAAATCCGTTTTAACTAGCGAACAGACCATGATGCTGGTTCTCAGTA
TCATGTCGCAGCGCCAACGCGACGAGTTTGAAAACACTCACGAATGCCAGTTTGCCTTGAGTGCCCACAGTCTTGGCCGG
TTCCGGGTCAGTGCGTTTACCCAACGCGACTCGGCCGGCATGGTGTTGCGCCGCATCGAAACCACCATCCCCAGCGCAGA
GGAATTGCACCTGCCGCCGGTGTTGAAAGAACTGATCATGTACAAGCGCGGTCTGGTGATGTTTGTCGGTGCCACCGGTA
CCGGTAAATCCACCTCGCTGGCGGCCTTGATTCGGCACCGCAACGAAAACAGCAGCGGCCATATCATTTCCATTGAAGAC
CCTATCGAGTTTATCCATCCGCACAAGGGCTGCATCATCACCCAGCGCGAAGTGGGCCTGGATACCGAATCGTTTGAAGT
GGCGTTAAAAAACACCCTGCGCCAAGCGCCGGACGTAATCCTGATTGGCGAGGTCAGAAGCCGGGACACCATGCAACATG
CGATCACCTTCGCCGAAACCGGGCATTTGTGCGTCTGTACCCTGCACGCCAATAACGCCAACCAGGCCTTAGACCGCATT
CTGCATTTTTTCCCGGAAGAAATGCACAGCCAGCTGTTCATGGATTTGTCTTTGAACTTGCGCGGTATCGTCGCCCAACA
ATTGATTCAGCGCGCCGACGGCAAGGGCCGCTACCCGGCTATTGAGATTTTGCTGAATACCCCGCTGGCATCAGACATGA
TCCGCAAGGGCGAAGTACACAAATTAAAAGAACTGATGAAAAGCTCCCGCGAGCATGGCATGCAGACTTTCGACCAAGCG
CTTTACGATCTGTATACGGCGGGTAAAATTGGTTACGATGACGCCTTGCACGCTGCCGACTCCCGCAACGAGGTGCGCCT
GATGATTAAACTGGGGGCCGAGAACGTCAACTTCGAAACCGCCGGCATGACCCTGGCCGAAAACGACGACGAGGGAGGCA
GTCTTTTTAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N0V4Z9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.929

100

0.676
  pilU Acinetobacter baylyi ADP1

64.789

94.164

0.61
  pilU Vibrio cholerae strain A1552

57.143

92.838

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.678

92.308

0.403
  pilT Legionella pneumophila strain Lp02

41.888

89.92

0.377
  pilT Legionella pneumophila strain ERS1305867

41.888

89.92

0.377
  pilT Acinetobacter nosocomialis M2

40.118

89.92

0.361
  pilT Acinetobacter baumannii D1279779

40.118

89.92

0.361
  pilT Acinetobacter baumannii strain A118

40.118

89.92

0.361

Multiple sequence alignment