Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VS_RS12630 Genome accession   NC_011753
Coordinates   2870342..2871379 (-) Length   345 a.a.
NCBI ID   WP_012604833.1    Uniprot ID   A0A2N7I159
Organism   Vibrio atlanticus     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2865342..2876379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VS_RS12605 (VS_2677) rsmE 2866034..2866765 (+) 732 WP_012604829.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VS_RS12610 (VS_2678) gshB 2866775..2867725 (+) 951 WP_012604830.1 glutathione synthase -
  VS_RS12615 (VS_2679) - 2867842..2868405 (+) 564 WP_012604831.1 YqgE/AlgH family protein -
  VS_RS12620 (VS_2680) ruvX 2868462..2868884 (+) 423 WP_009847698.1 Holliday junction resolvase RuvX -
  VS_RS12625 (VS_2681) pilU 2869218..2870327 (-) 1110 WP_041472921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VS_RS12630 (VS_2682) pilT 2870342..2871379 (-) 1038 WP_012604833.1 type IV pilus twitching motility protein PilT Machinery gene
  VS_RS12635 (VS_2683) - 2871406..2872122 (+) 717 WP_012604834.1 YggS family pyridoxal phosphate-dependent enzyme -
  VS_RS12640 (VS_2684) proC 2872186..2873004 (+) 819 WP_012604835.1 pyrroline-5-carboxylate reductase -
  VS_RS12645 (VS_2685) - 2873080..2873637 (+) 558 WP_012604836.1 YggT family protein -
  VS_RS12650 (VS_2686) yggU 2873667..2873957 (+) 291 WP_012604837.1 DUF167 family protein YggU -
  VS_RS12655 (VS_2687) - 2873986..2874417 (+) 432 WP_012604838.1 DUF4426 domain-containing protein -
  VS_RS12660 (VS_2688) - 2874466..2875065 (+) 600 WP_012604839.1 XTP/dITP diphosphatase -
  VS_RS12665 (VS_2689) hemW 2875065..2876252 (+) 1188 WP_012604840.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38227.75 Da        Isoelectric Point: 5.9011

>NTDB_id=32295 VS_RS12630 WP_012604833.1 2870342..2871379(-) (pilT) [Vibrio atlanticus]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=32295 VS_RS12630 WP_012604833.1 2870342..2871379(-) (pilT) [Vibrio atlanticus]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCCGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTAGTTTTTGAGA
TCATGGACGACTCACAGCGCGGTGAGTTTGAGGAGAAATTGGAAGTAGACTTCTCTTTTGAATTACCCAATGTTGGTCGA
TTCCGTGTTAATGCGTTTAACCAAGCTCGTGGGTGCGCAGCGGTCTTTCGAACCATCCCTGTAGAAATCCCAACGTTAGA
GCAGTTGGGCGCACCTGATATTTTTGAAAAAATTGCCAATTATGAAAAAGGCTTAGTGCTGGTGACTGGCCCTACGGGGT
CTGGTAAATCGACAACTTTGGCGGCGATGGTTGATTATGTAAACCGTAATCACAACAAACACATTTTGACCATTGAAGAC
CCAATTGAATTCGTTCACACCAATAATAAATGTCTGGTTAACCAGCGTGAAGTTCATCGTGATACGCACAGCTTTAAAGC
GGCGCTACGCAGTGCGTTACGTGAAGACCCGGACGTAATTCTTGTAGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGTTAACGGCCGCAGAAACTGGTCACCTAGTTTTTGGTACTTTGCATACTAGCTCTGCAGCAAAAACCGTAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGATATGGTTCGTTCGATGCTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
ACTATTAAAGCGTGTGGGTGGTGGTCGTGTCGCTTGTCATGAAATCATGATGGCGACACCTGCAATCAGAAACTTGATTC
GTGAAGATAAAGTCGCGCAGATGTACTCGATTATTCAAACGGGCGCGGCACATGGTATGCAGACCATGGAGCAAAACGCG
AAACAACTGATGGCACAAGGCTTGGTTGACTCGGAAGAGGTCGAGAAAAAGATTGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7I159

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.406

100

0.884
  pilT Vibrio cholerae strain A1552

88.406

100

0.884
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.797

100

0.658
  pilT Legionella pneumophila strain ERS1305867

65.797

100

0.658
  pilT Neisseria meningitidis 8013

63.478

100

0.635
  pilT Neisseria gonorrhoeae MS11

63.188

100

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.841

0.493
  pilU Vibrio cholerae strain A1552

43.385

94.203

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment