Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VS_RS12625 Genome accession   NC_011753
Coordinates   2869218..2870327 (-) Length   369 a.a.
NCBI ID   WP_041472921.1    Uniprot ID   A0A1C3IVG6
Organism   Vibrio atlanticus     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2864218..2875327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VS_RS12605 (VS_2677) rsmE 2866034..2866765 (+) 732 WP_012604829.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VS_RS12610 (VS_2678) gshB 2866775..2867725 (+) 951 WP_012604830.1 glutathione synthase -
  VS_RS12615 (VS_2679) - 2867842..2868405 (+) 564 WP_012604831.1 YqgE/AlgH family protein -
  VS_RS12620 (VS_2680) ruvX 2868462..2868884 (+) 423 WP_009847698.1 Holliday junction resolvase RuvX -
  VS_RS12625 (VS_2681) pilU 2869218..2870327 (-) 1110 WP_041472921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VS_RS12630 (VS_2682) pilT 2870342..2871379 (-) 1038 WP_012604833.1 type IV pilus twitching motility protein PilT Machinery gene
  VS_RS12635 (VS_2683) - 2871406..2872122 (+) 717 WP_012604834.1 YggS family pyridoxal phosphate-dependent enzyme -
  VS_RS12640 (VS_2684) proC 2872186..2873004 (+) 819 WP_012604835.1 pyrroline-5-carboxylate reductase -
  VS_RS12645 (VS_2685) - 2873080..2873637 (+) 558 WP_012604836.1 YggT family protein -
  VS_RS12650 (VS_2686) yggU 2873667..2873957 (+) 291 WP_012604837.1 DUF167 family protein YggU -
  VS_RS12655 (VS_2687) - 2873986..2874417 (+) 432 WP_012604838.1 DUF4426 domain-containing protein -
  VS_RS12660 (VS_2688) - 2874466..2875065 (+) 600 WP_012604839.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41454.32 Da        Isoelectric Point: 5.6712

>NTDB_id=32294 VS_RS12625 WP_041472921.1 2869218..2870327(-) (pilU) [Vibrio atlanticus]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQDRRDEYKQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=32294 VS_RS12625 WP_041472921.1 2869218..2870327(-) (pilU) [Vibrio atlanticus]
ATGGAATTGAATCAAATTCTTGAAGGGATGCTCTCTCAAAAGGCGTCGGATCTTTATATCACTGTCGATGCGCCAATCCT
TTTTCGTGTGGATGGTGAACTACGACCTCAGGGAGAGAAGTTGAATGCTGATCAAGTATCTCAGTTACTTGATGCAATGA
TGGATCAAGATCGACGCGATGAATACAAGCAAACGCGTGAGGCTAATTTTGCCATCGTGCGTGATTTTGGTCGTTTTCGT
GTCAGTGCATTCTTTCAGCGAGAGCTACCTGGAGCGGTAATTCGTCGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGACGTACTACAAGACCTTTCAATCGCTAAGCGCGGGCTTGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA
AATCGACCTCGATGGCTGCGATGACAGGCTATCGCAATACCAATCGTTCAGGTCATATCTTGACGGTTGAAGACCCGATT
GAATTCGTACACGAACATAAAAAGTGCATCGTGACTCAACGAGAGGTTGGACTCGACACCGAAAGCTATGAAGTCGCGCT
TAAAAACTCGTTACGCCAAGCCCCTGATATGATTTTGATTGGCGAAATCCGTAGTCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCAACCCTGCATGCCAATAACGCTAACCAGGCGCTAGAGCGTATTCTTCAC
TTGGTGCCGAAAGAGCAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAATCTGCGTGGTGTGGTCGCTCAGCAGTTAAT
ACGCGATAAAAATGGCAATGGTCGTCATGGTGTCTTCGAAATTCTGCTGAATAGCCCTCGTATATCTGACTTGATTCGTC
GTGGTGAGTTGCATGAGTTAAAAGCGACCATGGCCAAATCGAAAGAGATTGGTATGCAGACTTTTGACCAAGCTTTGTAT
GATTTAGTGGTTGCGGACAAGATTAGCGAGGATGATGCATTCCATAGTGCAGATTCTGCCAATGACTTGCGTTTAATGCT
CAAGACCAAACGTGGCGATGATGACTACGGAACTGGTGCGTTGGCTGGCGTTAAGATTGATATGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3IVG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

99.729

0.854
  pilU Pseudomonas stutzeri DSM 10701

55.795

100

0.561
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain ERS1305867

42.982

92.683

0.398
  pilT Legionella pneumophila strain Lp02

42.982

92.683

0.398
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

91.87

0.385
  pilT Pseudomonas aeruginosa PAK

41.493

90.786

0.377
  pilT Acinetobacter baylyi ADP1

42.638

88.347

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.597

90.786

0.369
  pilT Vibrio cholerae strain A1552

41.538

88.076

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.076

0.366

Multiple sequence alignment