Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EAG14_RS19750 Genome accession   NZ_CP033069
Coordinates   4447614..4448657 (+) Length   347 a.a.
NCBI ID   WP_099657637.1    Uniprot ID   A0A2G6YDT7
Organism   Acidovorax sp. 1608163     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4442614..4453657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAG14_RS19730 (EAG14_19730) - 4443151..4444452 (-) 1302 WP_121729893.1 DUF445 domain-containing protein -
  EAG14_RS19735 (EAG14_19735) - 4444472..4445611 (-) 1140 WP_121729894.1 aminotransferase class V-fold PLP-dependent enzyme -
  EAG14_RS19740 (EAG14_19740) ltaE 4445755..4446825 (+) 1071 WP_121729895.1 low-specificity L-threonine aldolase -
  EAG14_RS19745 (EAG14_19745) - 4446847..4447575 (-) 729 WP_121729896.1 YggS family pyridoxal phosphate-dependent enzyme -
  EAG14_RS19750 (EAG14_19750) pilT 4447614..4448657 (+) 1044 WP_099657637.1 type IV pilus twitching motility protein PilT Machinery gene
  EAG14_RS19755 (EAG14_19755) - 4448724..4449359 (+) 636 WP_099657636.1 cyclic nucleotide-binding domain-containing protein -
  EAG14_RS19760 (EAG14_19760) pilU 4449426..4450562 (+) 1137 WP_099657635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EAG14_RS19765 (EAG14_19765) - 4450700..4451614 (+) 915 WP_121729897.1 NAD(P)-dependent oxidoreductase -
  EAG14_RS19770 (EAG14_19770) - 4451749..4452405 (-) 657 WP_099657633.1 BON domain-containing protein -
  EAG14_RS19775 (EAG14_19775) - 4452402..4453004 (-) 603 WP_121729898.1 SIS domain-containing protein -
  EAG14_RS19780 (EAG14_19780) - 4453131..4453559 (-) 429 WP_121729899.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38189.98 Da        Isoelectric Point: 6.7745

>NTDB_id=321152 EAG14_RS19750 WP_099657637.1 4447614..4448657(+) (pilT) [Acidovorax sp. 1608163]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEALDHKTVHAMVYDIMSDSQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=321152 EAG14_RS19750 WP_099657637.1 4447614..4448657(+) (pilT) [Acidovorax sp. 1608163]
GTGGACATTACCCAATTGCTGGCATTCAGCGTCAAGAACAAGGCCTCCGACTTGCACTTGTCGGCGGGGCTTCCGCCCAT
GATCCGGGTGCACGGCGATGTGCGGCGCATCAACGTCGAGGCGCTGGACCACAAGACCGTGCACGCCATGGTGTACGACA
TCATGAGCGATTCCCAGCGCAAGCAATATGAGGAATTCCTGGAGGTTGACTTCTCGTTTGAGATCGAAGGCTTGGCCCGT
TTCCGTGTCAACGCATTCAACCAGAACCGTGGCGCAGCGGCCGTGTTCCGGACGATTCCGAGCAAGATCCTGACGCTGGA
GCAACTGAACGCCCCCAAGATTTTTGGCGACCTGGCCCTCAAGCCACGGGGCCTGGTGCTGGTGACCGGCCCTACGGGCT
CGGGCAAGTCCACCACGCTGGCCGCCATGGTCAACTACCTGAACGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGATGACGCTGTCGTTCGCCGC
TGCGCTCAAATCCGCACTGCGCGAAGACCCGGACGCGATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACGGCCGCTGAAACAGGCCACTTGGTGTTCGGCACGCTGCACACGTCCAGCGCCGCCAAGACCATCGACCGGATC
ATTGACGTGTTCCCGGCCGAAGAAAAGGAAATGGTCCGCGCGATGTTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAAACCAAGGACGGCTCGGGCCGCGTGGCTGCGCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTGGGCATGCAGACACTGGACCAGAAC
CTCACGGATCTGGTGCGCCGCAACATCATCAGCCCTGCAGAAGCGCGCGCCAAGGCCAAGATCCCCGAGAACTTCCCTGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G6YDT7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment