Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EAG14_RS19760 Genome accession   NZ_CP033069
Coordinates   4449426..4450562 (+) Length   378 a.a.
NCBI ID   WP_099657635.1    Uniprot ID   -
Organism   Acidovorax sp. 1608163     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4444426..4455562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAG14_RS19735 (EAG14_19735) - 4444472..4445611 (-) 1140 WP_121729894.1 aminotransferase class V-fold PLP-dependent enzyme -
  EAG14_RS19740 (EAG14_19740) ltaE 4445755..4446825 (+) 1071 WP_121729895.1 low-specificity L-threonine aldolase -
  EAG14_RS19745 (EAG14_19745) - 4446847..4447575 (-) 729 WP_121729896.1 YggS family pyridoxal phosphate-dependent enzyme -
  EAG14_RS19750 (EAG14_19750) pilT 4447614..4448657 (+) 1044 WP_099657637.1 type IV pilus twitching motility protein PilT Machinery gene
  EAG14_RS19755 (EAG14_19755) - 4448724..4449359 (+) 636 WP_099657636.1 cyclic nucleotide-binding domain-containing protein -
  EAG14_RS19760 (EAG14_19760) pilU 4449426..4450562 (+) 1137 WP_099657635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EAG14_RS19765 (EAG14_19765) - 4450700..4451614 (+) 915 WP_121729897.1 NAD(P)-dependent oxidoreductase -
  EAG14_RS19770 (EAG14_19770) - 4451749..4452405 (-) 657 WP_099657633.1 BON domain-containing protein -
  EAG14_RS19775 (EAG14_19775) - 4452402..4453004 (-) 603 WP_121729898.1 SIS domain-containing protein -
  EAG14_RS19780 (EAG14_19780) - 4453131..4453559 (-) 429 WP_121729899.1 YraN family protein -
  EAG14_RS19785 (EAG14_19785) rsmI 4453559..4454491 (+) 933 WP_121729900.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41875.28 Da        Isoelectric Point: 6.9660

>NTDB_id=321153 EAG14_RS19760 WP_099657635.1 4449426..4450562(+) (pilU) [Acidovorax sp. 1608163]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTPTHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFVQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEVTMAKRGLCIMVGATGSGKSTTLAAMVDWRNEHSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGMVSQRLIPKQDGKGRAAAVEIMLNTPLISDLIFKGDVSEIKEIMKKSRNLG
MQTFDQSLFDLYEANVISYEDALRNADSLNDLRLQIKLNSQRAKSPDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=321153 EAG14_RS19760 WP_099657635.1 4449426..4450562(+) (pilU) [Acidovorax sp. 1608163]
ATGGAACGCGATCAGGCCAGTAAATTTATCAATGACCTGCTCAAGTTGATGGTCAGCCGCAACGGCAGCGACTTGTTCAT
CACAGCGGAGTTTCCACCGGCCATCAAGGTCGATGGCAAGGTGACCAAGGTATCGCCCCAGCCTTTGACGCCCACGCACA
CCCTGACCTTGGCCCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTTGAACGCACCAAGGAATGCAACTTCGCCATT
TCGCCTGCGGGCATTGGTCGCTTCCGGGTGAATGCCTTTGTGCAGCAAGGCCGGGTCGGCATGGTGCTGCGGACCATTCC
GCTGACACTGCCCACCATTGACGGTCTGGGGGTGCCCCAGGTGCTCAAGGAAGTGACGATGGCCAAGCGCGGCTTGTGCA
TCATGGTGGGCGCTACCGGCTCCGGCAAGTCCACCACGCTGGCCGCGATGGTGGACTGGCGCAACGAGCACTCTTTTGGC
CACATCATCACGGTGGAAGACCCGGTCGAATTCGTGCACCCCCACAAGAACTGCGTAGTGACGCAGCGCGAAGTGGGCCT
GGACACCGACAGCTGGGAAGCCGCCCTCAAGAACACCCTGCGCCAAGCGCCCGATGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCATGCCGTGGCCTTCGCTGAAACCGGGCACTTGTGCCTGGCCACATTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCAGAAGAGCGCCGCGCCCAATTGCTGATGGACTTGTCGCTGAACTT
GCGCGGCATGGTGTCACAGCGGCTGATTCCCAAGCAAGACGGCAAGGGCCGTGCTGCGGCGGTGGAAATCATGCTCAACA
CCCCGCTGATTTCTGACCTGATCTTCAAGGGCGATGTCTCTGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGTCGCTCTTCGATCTGTATGAAGCGAACGTCATCAGCTATGAAGACGCGTTGCGCAACGCAGA
CTCCTTGAACGACCTGCGCTTGCAAATCAAGCTCAACAGCCAACGCGCCAAGTCACCCGACTTGGCCTCGGGCACCGAGC
ATTTCGCCATAGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.751

92.328

0.579
  pilU Acinetobacter baylyi ADP1

57.983

94.444

0.548
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment