Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TMZ1T_RS17955 Genome accession   NC_011662
Coordinates   4068908..4069951 (+) Length   347 a.a.
NCBI ID   WP_004309695.1    Uniprot ID   N6XTK2
Organism   Thauera aminoaromatica     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4063908..4074951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMZ1T_RS17930 (Tmz1t_3698) - 4064253..4065212 (+) 960 WP_012586075.1 aspartate carbamoyltransferase catalytic subunit -
  TMZ1T_RS17935 (Tmz1t_3699) - 4065246..4066523 (+) 1278 WP_012586076.1 dihydroorotase -
  TMZ1T_RS17940 (Tmz1t_3700) - 4066615..4067196 (-) 582 WP_012586077.1 YggT family protein -
  TMZ1T_RS17945 (Tmz1t_3701) proC 4067200..4068024 (-) 825 WP_012586078.1 pyrroline-5-carboxylate reductase -
  TMZ1T_RS17950 (Tmz1t_3702) - 4068068..4068781 (-) 714 WP_012586079.1 YggS family pyridoxal phosphate-dependent enzyme -
  TMZ1T_RS17955 (Tmz1t_3703) pilT 4068908..4069951 (+) 1044 WP_004309695.1 type IV pilus twitching motility protein PilT Machinery gene
  TMZ1T_RS17960 (Tmz1t_3704) pilU 4069993..4071129 (+) 1137 WP_004309696.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TMZ1T_RS17965 (Tmz1t_3705) - 4071151..4072314 (-) 1164 WP_012586080.1 class I SAM-dependent RNA methyltransferase -
  TMZ1T_RS17970 (Tmz1t_3706) - 4072491..4073513 (-) 1023 WP_012586081.1 formate dehydrogenase subunit gamma -
  TMZ1T_RS17975 (Tmz1t_3707) - 4073524..4073760 (-) 237 WP_004323744.1 hypothetical protein -
  TMZ1T_RS17980 (Tmz1t_3708) fdh3B 4073757..4074395 (-) 639 WP_004323743.1 formate dehydrogenase FDH3 subunit beta -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38602.38 Da        Isoelectric Point: 7.0603

>NTDB_id=32021 TMZ1T_RS17955 WP_004309695.1 4068908..4069951(+) (pilT) [Thauera aminoaromatica]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKEVHAMVYDIMNDAQRKQLEETWECDFSFAVPNLAR
FRVNAFQQNRGAGAVFRTIPSKVLTLEELNCPKIFKEIAHQPRGIVLVTGPTGSGKSTTLAAMLDYVNENEYGHILTVED
PIEFVHESKRCLVNQREVHRDTMSFNNALRAALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNIGMQTLDQC
LVDLVRRNVVSAAEARVKAQNKDSFAA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=32021 TMZ1T_RS17955 WP_004309695.1 4068908..4069951(+) (pilT) [Thauera aminoaromatica]
ATGGACATCACCGAACTGCTCGCCTTCGCGGTCAAGAACAAGGCCTCCGACCTCCACCTCTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGGATCAACCTGCCGCCGCTCGAGCACAAGGAGGTGCATGCCATGGTGTACGACA
TCATGAACGACGCGCAGCGCAAGCAGCTCGAGGAGACCTGGGAGTGCGACTTCTCCTTCGCCGTGCCCAACCTCGCGCGC
TTCCGCGTCAACGCCTTCCAGCAGAACCGCGGCGCAGGCGCGGTGTTCCGCACCATTCCGAGCAAGGTGCTGACCCTGGA
GGAGCTCAACTGCCCGAAGATCTTCAAGGAGATCGCCCACCAGCCGCGCGGCATCGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGCTCGACTACGTGAACGAAAACGAGTACGGCCACATCCTCACCGTGGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGCGCTGCCTGGTGAACCAGCGCGAGGTGCACCGCGACACCATGTCCTTCAACAA
CGCGCTGCGCGCTGCGCTGCGCGAGGACCCCGACGTGATCCTGGTGGGCGAGATGCGCGACCTCGAGACCATCCGCCTCG
CCCTCACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCATCCGCGGCCAAGACCATCGACCGGATC
GTCGACGTCTTCCCCGCGGCCGAGAAGGACATGGTGCGCTCGATGCTTTCGGAATCCCTGCGCGCAGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCCAGGGCCGGGTCGCGGCGCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTCA
TCCGCGAGAACAAGATCGCGCAGATGTATTCCTCGATCCAGACCGGCCAGAATATCGGCATGCAGACCCTCGACCAGTGC
CTCGTCGACCTGGTCCGGCGCAACGTCGTGTCGGCCGCGGAAGCACGCGTGAAGGCGCAGAACAAGGACAGCTTCGCCGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6XTK2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.71

99.424

0.793
  pilT Acinetobacter baumannii D1279779

79.71

99.424

0.793
  pilT Acinetobacter baumannii strain A118

79.71

99.424

0.793
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

76.812

99.424

0.764
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

70.231

99.712

0.7
  pilT Neisseria gonorrhoeae MS11

69.942

99.712

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

97.983

0.671
  pilT Vibrio cholerae strain A1552

68.529

97.983

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.312

99.712

0.522
  pilU Acinetobacter baylyi ADP1

43.195

97.406

0.421
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Pseudomonas stutzeri DSM 10701

40.87

99.424

0.406

Multiple sequence alignment