Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TMZ1T_RS17960 Genome accession   NC_011662
Coordinates   4069993..4071129 (+) Length   378 a.a.
NCBI ID   WP_004309696.1    Uniprot ID   N6YRT2
Organism   Thauera aminoaromatica     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4064993..4076129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMZ1T_RS17935 (Tmz1t_3699) - 4065246..4066523 (+) 1278 WP_012586076.1 dihydroorotase -
  TMZ1T_RS17940 (Tmz1t_3700) - 4066615..4067196 (-) 582 WP_012586077.1 YggT family protein -
  TMZ1T_RS17945 (Tmz1t_3701) proC 4067200..4068024 (-) 825 WP_012586078.1 pyrroline-5-carboxylate reductase -
  TMZ1T_RS17950 (Tmz1t_3702) - 4068068..4068781 (-) 714 WP_012586079.1 YggS family pyridoxal phosphate-dependent enzyme -
  TMZ1T_RS17955 (Tmz1t_3703) pilT 4068908..4069951 (+) 1044 WP_004309695.1 type IV pilus twitching motility protein PilT Machinery gene
  TMZ1T_RS17960 (Tmz1t_3704) pilU 4069993..4071129 (+) 1137 WP_004309696.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TMZ1T_RS17965 (Tmz1t_3705) - 4071151..4072314 (-) 1164 WP_012586080.1 class I SAM-dependent RNA methyltransferase -
  TMZ1T_RS17970 (Tmz1t_3706) - 4072491..4073513 (-) 1023 WP_012586081.1 formate dehydrogenase subunit gamma -
  TMZ1T_RS17975 (Tmz1t_3707) - 4073524..4073760 (-) 237 WP_004323744.1 hypothetical protein -
  TMZ1T_RS17980 (Tmz1t_3708) fdh3B 4073757..4074395 (-) 639 WP_004323743.1 formate dehydrogenase FDH3 subunit beta -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42288.74 Da        Isoelectric Point: 6.9714

>NTDB_id=32022 TMZ1T_RS17960 WP_004309696.1 4069993..4071129(+) (pilU) [Thauera aminoaromatica]
MERDQALKFMHDLLRLMLQKNGSDLFITAGFPPAIKVDGRIVPQSNQILQPQHTTELARAIMNDRQAADFETHKECNFGI
SPAGIGRFRANAFVQQGRVALVLRTIPQKIPNFKELGLPPVLTEIALAKRGLVIFVGGTGTGKTTSLASLVDYRNENTYG
HIITIEDPIEFVHQHKNCIVSQREIGIDTESWMSALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPMKDRKGRVPAVEVMLNSPLIADLVFKGNVPEIKDVMKRSRELG
MQTFDQSLFQLYEDDLIGYEDALRNADSVNDLRLQIKLNSRHGDPLAAGGGIQNLGIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=32022 TMZ1T_RS17960 WP_004309696.1 4069993..4071129(+) (pilU) [Thauera aminoaromatica]
ATGGAACGCGACCAGGCCCTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCGGATCTCTTCAT
CACCGCCGGCTTCCCGCCTGCGATCAAGGTCGACGGCCGCATCGTGCCGCAGTCGAACCAGATCCTGCAGCCGCAGCACA
CCACCGAGCTGGCGCGCGCGATCATGAACGACCGCCAGGCCGCCGACTTCGAGACCCACAAGGAGTGCAACTTCGGCATC
TCGCCGGCGGGCATCGGCCGCTTCCGCGCCAATGCCTTCGTGCAGCAGGGCCGCGTCGCCCTGGTGCTGCGCACCATCCC
GCAGAAGATCCCCAATTTCAAGGAGCTCGGCCTGCCGCCGGTGCTCACCGAGATCGCGCTCGCCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACCACCTCGCTCGCATCCCTGGTCGATTACCGCAACGAGAACACCTACGGC
CACATCATCACCATCGAGGACCCGATCGAGTTCGTGCACCAGCACAAGAACTGCATCGTGTCCCAGCGCGAGATCGGCAT
CGACACCGAGTCCTGGATGTCTGCGCTCAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACAATGGACTACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTTCACGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAATTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGATGGTCTCGCAGCGCCTGCTGCCGATGAAGGACCGCAAGGGCCGCGTACCGGCGGTGGAGGTGATGCTCAACT
CGCCGCTGATCGCCGACCTGGTCTTCAAGGGCAACGTGCCCGAGATCAAGGACGTGATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCCAGCTCTACGAGGACGACCTGATCGGCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCGCCACGGCGACCCCCTCGCGGCCGGCGGCGGCATCCAGA
ACCTCGGCATCCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6YRT2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

92.063

0.582
  pilU Acinetobacter baylyi ADP1

56.583

94.444

0.534
  pilU Vibrio cholerae strain A1552

53.463

95.503

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.776

88.624

0.397
  pilT Legionella pneumophila strain Lp02

43.202

87.566

0.378
  pilT Legionella pneumophila strain ERS1305867

43.202

87.566

0.378
  pilT Acinetobacter baylyi ADP1

41.543

89.153

0.37
  pilT Pseudomonas aeruginosa PAK

41.791

88.624

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368
  pilT Acinetobacter nosocomialis M2

41.692

87.566

0.365
  pilT Acinetobacter baumannii D1279779

41.692

87.566

0.365
  pilT Acinetobacter baumannii strain A118

41.692

87.566

0.365

Multiple sequence alignment