Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E2348C_RS17170 Genome accession   NC_011601
Coordinates   3322439..3323419 (-) Length   326 a.a.
NCBI ID   WP_012578995.1    Uniprot ID   A0A376HK34
Organism   Escherichia coli O127:H6 str. E2348/69     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3317439..3328419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2348C_RS17140 (E2348_C_3197) yggI 3318272..3318769 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  E2348C_RS17145 (E2348_C_3198) endA 3318864..3319571 (+) 708 WP_000286507.1 deoxyribonuclease I -
  E2348C_RS17150 (E2348_C_3199) rsmE 3319650..3320381 (+) 732 WP_012578994.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E2348C_RS17155 (E2348_C_3200) gshB 3320394..3321344 (+) 951 WP_000593260.1 glutathione synthase -
  E2348C_RS17160 (E2348_C_3201) yqgE 3321453..3322016 (+) 564 WP_001339287.1 YqgE/AlgH family protein -
  E2348C_RS17165 (E2348_C_3202) ruvX 3322016..3322432 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  E2348C_RS17170 (E2348_C_3203) pilT 3322439..3323419 (-) 981 WP_012578995.1 type IV pilus twitching motility protein PilT Machinery gene
  E2348C_RS17175 (E2348_C_3204) yggS 3323437..3324141 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  E2348C_RS17180 (E2348_C_3205) yggT 3324159..3324725 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  E2348C_RS17185 (E2348_C_3206) yggU 3324722..3325012 (+) 291 WP_001277194.1 DUF167 family protein YggU -
  E2348C_RS17190 (E2348_C_3207) rdgB 3325020..3325613 (+) 594 WP_001174747.1 XTP/dITP diphosphatase -
  E2348C_RS17195 (E2348_C_3208) hemW 3325606..3326742 (+) 1137 WP_000239938.1 radical SAM family heme chaperone HemW -
  E2348C_RS17200 (E2348_C_3209) yggM 3326807..3327814 (-) 1008 WP_000745186.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35929.12 Da        Isoelectric Point: 5.5860

>NTDB_id=31933 E2348C_RS17170 WP_012578995.1 3322439..3323419(-) (pilT) [Escherichia coli O127:H6 str. E2348/69]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=31933 E2348C_RS17170 WP_012578995.1 3322439..3323419(-) (pilT) [Escherichia coli O127:H6 str. E2348/69]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGCCCGCAGCTCGAACAACTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACTGGGCAACAAGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A376HK34

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment