Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AFLV_RS03535 Genome accession   NC_011567
Coordinates   677422..678456 (+) Length   344 a.a.
NCBI ID   WP_041638021.1    Uniprot ID   -
Organism   Anoxybacillus flavithermus WK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672422..683456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFLV_RS03515 (Aflv_0621) - 672565..673752 (+) 1188 WP_041638013.1 hypothetical protein -
  AFLV_RS03520 (Aflv_0622) - 673749..674495 (+) 747 WP_041638019.1 PRC-barrel domain-containing protein -
  AFLV_RS03525 (Aflv_0623) - 674509..675753 (+) 1245 WP_041638020.1 VanW family protein -
  AFLV_RS03530 (Aflv_0624) - 675750..677411 (+) 1662 WP_012574311.1 ATPase, T2SS/T4P/T4SS family -
  AFLV_RS03535 (Aflv_0625) pilT 677422..678456 (+) 1035 WP_041638021.1 type IV pilus twitching motility protein PilT Machinery gene
  AFLV_RS03540 (Aflv_0626) - 678463..679674 (+) 1212 WP_012574313.1 type II secretion system F family protein -
  AFLV_RS03545 (Aflv_0627) - 679750..680289 (+) 540 WP_012574314.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AFLV_RS03550 (Aflv_0628) comC 680330..681079 (+) 750 WP_012574315.1 A24 family peptidase Machinery gene
  AFLV_RS03555 (Aflv_0629) pilM 681092..682060 (+) 969 WP_012574316.1 pilus assembly protein PilM -
  AFLV_RS03560 (Aflv_0630) - 682044..682625 (+) 582 WP_041638022.1 PilN domain-containing protein -
  AFLV_RS03565 (Aflv_0631) pilO 682622..683248 (+) 627 WP_041638023.1 type 4a pilus biogenesis protein PilO -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37904.85 Da        Isoelectric Point: 8.0148

>NTDB_id=31829 AFLV_RS03535 WP_041638021.1 677422..678456(+) (pilT) [Anoxybacillus flavithermus WK1]
MKQKVDAMLRAAFELKASDIHLTVGIPPIFRVNGDLKRYGQDALSSADTEQMAKAIVPENMWHHVEENGELDLSYSLHGV
SRFRVNVFKQRGCISLAIRIVPTKIPTLEELQLPDVLKKIVTKPQGLVLVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYVHKHGSCVIDQREVGTDTNNFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVFGTLHTSSAPATID
RIIDVFQPEQQTQIRIQLATVLVAIISQRLFPRAQKNGRIAATEILINNAPVANLIRNGKVHQIPSVMQTSRALGMHTLA
ANIKELVQQGLVAKEAVEPYLQEG

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=31829 AFLV_RS03535 WP_041638021.1 677422..678456(+) (pilT) [Anoxybacillus flavithermus WK1]
ATGAAACAAAAAGTAGATGCGATGTTGCGTGCTGCATTTGAATTAAAAGCTTCTGATATTCATTTAACAGTCGGTATTCC
TCCGATTTTTCGCGTGAACGGAGATTTGAAACGTTACGGGCAAGACGCGTTGTCTTCGGCAGATACAGAACAAATGGCAA
AAGCGATCGTTCCTGAAAACATGTGGCACCATGTTGAGGAAAACGGGGAGCTAGATTTATCTTATAGCCTCCATGGTGTT
TCGCGTTTTCGTGTCAACGTGTTTAAACAGCGTGGGTGTATATCGCTTGCCATTCGTATTGTGCCAACGAAAATTCCAAC
GCTTGAAGAGTTGCAATTGCCAGATGTGTTAAAAAAGATCGTTACAAAACCGCAAGGACTTGTTCTTGTGACAGGACCGA
CGGGGAGCGGAAAATCGACAACGTTAGCTGCGATGATTGATTATATGAATAAAACGATGCGCAAACATATTATTACGCTT
GAAGATCCGATTGAATATGTGCATAAACATGGAAGTTGCGTTATCGACCAGCGCGAAGTCGGAACGGATACAAACAATTT
CGCAAACGGTTTGCGCGCCGCGCTTCGTCAAGATCCAGACGTCATTTTAGTCGGGGAAATGCGTGATTTAGAAACGATTC
AAACGGCAATTACCGCTGCAGAAACAGGACATCTCGTATTCGGAACGCTGCATACGTCAAGTGCTCCTGCGACGATTGAC
CGAATCATCGACGTATTTCAACCAGAACAACAAACGCAAATTCGCATTCAATTGGCAACCGTTTTAGTTGCTATTATTTC
ACAACGACTATTTCCGCGCGCACAAAAAAACGGGAGAATTGCGGCCACCGAAATTTTGATTAACAATGCGCCCGTTGCCA
ATTTAATTCGCAACGGAAAAGTTCATCAAATTCCAAGTGTGATGCAAACGAGTCGTGCGCTCGGCATGCATACGCTTGCG
GCGAACATTAAAGAACTTGTTCAACAAGGGCTCGTCGCGAAAGAAGCAGTCGAGCCGTATTTGCAGGAAGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.522

97.965

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

97.674

0.488
  pilT Vibrio cholerae strain A1552

50

97.674

0.488
  pilT Pseudomonas aeruginosa PAK

50.613

94.767

0.48
  pilT Pseudomonas stutzeri DSM 10701

49.848

95.64

0.477
  pilT Neisseria meningitidis 8013

50.938

93.023

0.474
  pilT Acinetobacter baumannii D1279779

50.309

94.186

0.474
  pilT Acinetobacter nosocomialis M2

50.309

94.186

0.474
  pilT Acinetobacter baumannii strain A118

50.309

94.186

0.474
  pilT Neisseria gonorrhoeae MS11

50.779

93.314

0.474
  pilT Acinetobacter baylyi ADP1

49.846

94.477

0.471
  pilT Legionella pneumophila strain ERS1305867

47.962

92.733

0.445
  pilT Legionella pneumophila strain Lp02

47.962

92.733

0.445
  pilU Pseudomonas stutzeri DSM 10701

42.771

96.512

0.413
  pilU Vibrio cholerae strain A1552

41.284

95.058

0.392
  pilU Acinetobacter baylyi ADP1

40.656

88.663

0.36

Multiple sequence alignment