Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D6Z43_RS18715 Genome accession   NZ_CP032616
Coordinates   3968167..3969201 (-) Length   344 a.a.
NCBI ID   WP_028629028.1    Uniprot ID   -
Organism   Pseudomonas sp. DY-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3963167..3974201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6Z43_RS18695 (D6Z43_18695) - 3963817..3965001 (-) 1185 WP_120653583.1 PLP-dependent aspartate aminotransferase family protein -
  D6Z43_RS18700 (D6Z43_18700) - 3964998..3966377 (-) 1380 WP_120653584.1 pyridoxal-phosphate dependent enzyme -
  D6Z43_RS18705 (D6Z43_18705) - 3966523..3966918 (-) 396 WP_120653585.1 TM2 domain-containing protein -
  D6Z43_RS18710 (D6Z43_18710) pilU 3966994..3968139 (-) 1146 WP_120653586.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D6Z43_RS18715 (D6Z43_18715) pilT 3968167..3969201 (-) 1035 WP_028629028.1 type IV pilus twitching motility protein PilT Machinery gene
  D6Z43_RS18720 (D6Z43_18720) - 3969365..3970060 (+) 696 WP_120653587.1 YggS family pyridoxal phosphate-dependent enzyme -
  D6Z43_RS18725 (D6Z43_18725) proC 3970071..3970889 (+) 819 WP_120653588.1 pyrroline-5-carboxylate reductase -
  D6Z43_RS18730 (D6Z43_18730) - 3970900..3971493 (+) 594 WP_120653589.1 YggT family protein -
  D6Z43_RS18735 (D6Z43_18735) - 3971501..3971797 (+) 297 WP_120653590.1 DUF167 domain-containing protein -
  D6Z43_RS18740 (D6Z43_18740) - 3972008..3973996 (+) 1989 WP_120653591.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37901.68 Da        Isoelectric Point: 6.8361

>NTDB_id=317054 D6Z43_RS18715 WP_028629028.1 3968167..3969201(-) (pilT) [Pseudomonas sp. DY-1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLVSRESAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=317054 D6Z43_RS18715 WP_028629028.1 3968167..3969201(-) (pilT) [Pseudomonas sp. DY-1]
ATGGACATTACTGAGCTGCTTGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCATCTCTCGGCCGGCCTGCCACCCAT
GATTCGCGTCGATGGCGACGTGCGCCGGATCAACCTTCCGGCCCTGGACCACAAGCAGGTCCACGCGTTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTCCTCGAGACAGACTTCTCCTTCGAAGTCCCGGGCGTCGCACGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCTTCCAAGGTGCTGAGCATGGA
GGACCTGGGGATGGGCGAGATCTTCAAGAAGATCTCAGACGTTCCCCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGCTCGACTACCTGAACAGCACCAAGTACCACCACATCCTCACCGTGGAAGAT
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGATCAACCAGCGGGAAGTGCACCGCGATACGCTCGGCTTCAACGA
AGCCCTGCGCTCGGCACTGCGTGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTATTCCCCGCCGAGGAAAAGTCCATGGTCCGCTCCATGCTCTCCGAGTCCCTGCAGTCGGTGATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGGGTGGCGGCTCACGAGATCATGATCGGCACCCCGGCAATCCGTAACCTGATTC
GCGAAGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCGGCTCGCTCGGCATGCAGACCCTCGACATGTGCCTC
AAGGGGCTTGTCGCCAAGGGACTGGTCAGTCGTGAAAGCGCGCGGGAAAAGGCCAAGAGCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.349

100

0.953
  pilT Pseudomonas stutzeri DSM 10701

94.477

100

0.945
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392

Multiple sequence alignment