Detailed information NTG0317053

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: D6Z43_RS18710
• Genome accession: NZ_CP032616
• Coordinates: 3966994..3968139 (-)
• Length: 381 a.a.
• NCBI ID: WP_120653586.1
• Uniprot ID: -
• Organism: Pseudomonas sp. DY-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3961994..3973139

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D6Z43_RS18695 (D6Z43_18695)	-	3963817..3965001 (-)	1185	WP_120653583.1	PLP-dependent aspartate aminotransferase family protein	-
D6Z43_RS18700 (D6Z43_18700)	-	3964998..3966377 (-)	1380	WP_120653584.1	pyridoxal-phosphate dependent enzyme	-
D6Z43_RS18705 (D6Z43_18705)	-	3966523..3966918 (-)	396	WP_120653585.1	TM2 domain-containing protein	-
D6Z43_RS18710 (D6Z43_18710)	pilU	3966994..3968139 (-)	1146	WP_120653586.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
D6Z43_RS18715 (D6Z43_18715)	pilT	3968167..3969201 (-)	1035	WP_028629028.1	type IV pilus twitching motility protein PilT	Machinery gene
D6Z43_RS18720 (D6Z43_18720)	-	3969365..3970060 (+)	696	WP_120653587.1	YggS family pyridoxal phosphate-dependent enzyme	-
D6Z43_RS18725 (D6Z43_18725)	proC	3970071..3970889 (+)	819	WP_120653588.1	pyrroline-5-carboxylate reductase	-
D6Z43_RS18730 (D6Z43_18730)	-	3970900..3971493 (+)	594	WP_120653589.1	YggT family protein	-
D6Z43_RS18735 (D6Z43_18735)	-	3971501..3971797 (+)	297	WP_120653590.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42629.97 Da
• Isoelectric Point: 6.6393

>NTDB_id=317053 D6Z43_RS18710 WP_120653586.1 3966994..3968139(-) (pilU) [Pseudomonas sp. DY-1]
MEFEKLLRLMVEKGGSDLFVTAGVPPSMKVNGKIMPVTKNPMSPEMTRETVMGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETTIPTIDELKLPEILKQLSLTKRGLVLFVGATGTGKSTSLAAMIGHRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAIAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLVADLIRKGEVHELKGLMKRSTEHGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLQHMSDGLSLQVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=317053 D6Z43_RS18710 WP_120653586.1 3966994..3968139(-) (pilU) [Pseudomonas sp. DY-1]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGTTCCGACCTCTTCGTCACGGCGGGCGTGCCGCCATC
GATGAAGGTCAATGGCAAGATCATGCCGGTGACCAAGAACCCCATGTCGCCGGAAATGACGCGGGAAACCGTGATGGGCG
TGATGAACGAACAGCAGCGGCGCGACTTCGCAGAGAACCACGAGTGCAACTTCGCCATCAGTGCGCGGGGCATTGGTCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTAGGCATGGTGTTGCGTCGGATCGAGACCACCATCCCCACCATCGA
CGAGCTGAAGCTGCCGGAAATCCTCAAGCAGCTGTCGCTGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGTA
CCGGTAAGTCCACCTCTCTGGCGGCGATGATCGGCCACCGCAACAAGAACAGCAGCGGTCACATCATTTCCATCGAGGAC
CCGATCGAGTTCATCCACCAGCATCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGATACCGAATCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCAGACGTGATCCTGATCGGCGAAGTGCGGACCCGCGAGACCATGGACCACG
CCATCGCCTTTGCGGAAACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGTATC
ATCAACTTCTTCCCCGCCGATCGGCAGCAGCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGTCGCGCGGTGATCGAAGTACTGCTGAACACGCCGCTGGTCGCCGATCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGGGCCTGATGAAACGCTCTACCGAACACGGCATGCAGACCTTCGACCAGGCG
CTCTACAACCTCTATTCCCAGGGCGAGATCACCTACGAAGATGCGCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAACCGATGGTGAGCACCTGCAGCACATGAGCGATGGTCTGTCGCTGCAGGTGAGCGAAG
AAGATCCGGGGCGCCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	90.289	100	0.903
pilU	Acinetobacter baylyi ADP1	64.146	93.701	0.601
pilU	Vibrio cholerae strain A1552	58.286	91.864	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.94	88.189	0.396