Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBEM_RS12950 Genome accession   NC_011146
Coordinates   3004302..3005402 (-) Length   366 a.a.
NCBI ID   WP_012531023.1    Uniprot ID   B5EGW9
Organism   Citrifermentans bemidjiense Bem     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2999302..3010402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBEM_RS12930 (Gbem_2590) - 2999491..2999721 (-) 231 WP_012531019.1 type IV pilin protein -
  GBEM_RS12935 (Gbem_2591) - 2999943..3001316 (-) 1374 WP_012531020.1 sigma-54 dependent transcriptional regulator -
  GBEM_RS12940 (Gbem_2592) - 3001414..3003033 (-) 1620 WP_012531021.1 nitrogen regulation protein NR(II) -
  GBEM_RS12945 (Gbem_2593) - 3003038..3004261 (-) 1224 WP_012531022.1 type II secretion system F family protein -
  GBEM_RS12950 (Gbem_2594) pilT 3004302..3005402 (-) 1101 WP_012531023.1 type IV pilus twitching motility protein PilT Machinery gene
  GBEM_RS12955 (Gbem_2595) pilB 3005418..3007124 (-) 1707 WP_012531024.1 type IV-A pilus assembly ATPase PilB -
  GBEM_RS12960 (Gbem_2596) aroE 3007180..3008052 (-) 873 WP_012531025.1 shikimate dehydrogenase -
  GBEM_RS12965 (Gbem_2597) - 3008052..3009911 (-) 1860 WP_012531026.1 DUF2723 domain-containing protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40906.37 Da        Isoelectric Point: 7.4857

>NTDB_id=31450 GBEM_RS12950 WP_012531023.1 3004302..3005402(-) (pilT) [Citrifermentans bemidjiense Bem]
MINFHQMLKELVERNGSDLHITTNTSPQIRIDGKLTPMDIPPLTPIETKQLCYSILTDSQKHKFEEENELDLSFGVKGLS
RFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVVRTLCDKPRGLILVTGPTGSGKSTTLASMIDRINLERHDHIVTVE
DPIEYLHPHKGCIVNQREVGADTKSFKHALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTMNR
IIDVFPPYQQTQVRTQLSFVLEGVMSQTLIPKANGGGRALALEVMVPNPAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QCLMNLLAKRIITVEDALGRSSEPDELKQMLAPGAGAGQMRRPPQR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=31450 GBEM_RS12950 WP_012531023.1 3004302..3005402(-) (pilT) [Citrifermentans bemidjiense Bem]
ATGATCAACTTTCACCAAATGCTGAAGGAACTGGTGGAACGTAACGGTTCCGACCTGCACATAACCACCAACACCTCGCC
GCAGATCCGCATCGACGGCAAGCTTACCCCGATGGACATCCCGCCTCTGACCCCCATCGAGACCAAGCAGCTCTGCTACA
GCATCCTGACCGACTCGCAAAAGCATAAGTTCGAGGAGGAGAACGAGCTCGACCTCTCCTTCGGCGTCAAGGGGCTATCG
CGCTTCAGGGGGAACATCTTCGTGCAGCGCGGCGCCGTCGCCGGGGTCTTCAGGGTGATCCCCTACAAGATCCTCACCTT
CGAGGAACTGGGGCTTCCGCCGGTAGTGAGGACGCTTTGCGACAAGCCGCGTGGCCTGATCCTCGTTACCGGCCCTACCG
GCAGCGGCAAGTCCACCACACTCGCCTCGATGATCGACCGGATCAACCTGGAGCGCCACGACCACATCGTGACCGTCGAG
GACCCGATCGAGTACCTGCACCCGCACAAGGGATGCATCGTGAACCAGCGCGAGGTCGGGGCGGACACCAAGAGTTTCAA
GCACGCGCTCAAGTACGTGCTGCGCCAGGACCCGGACGTGGTGCTGATCGGGGAGTTGCGCGACCTGGAGACCATCGAGG
CAGCGCTTACCCTCGCCGAAACCGGCCACCTCTGCTTCGCGACCCTGCACACCAACTCCTGCGCCCAGACCATGAACAGG
ATCATCGACGTCTTCCCTCCCTACCAGCAGACCCAGGTGCGCACGCAGCTCTCCTTTGTGCTCGAAGGGGTCATGTCGCA
GACCCTGATACCGAAAGCAAACGGCGGGGGGAGGGCGCTCGCCCTCGAGGTCATGGTTCCCAATCCCGCCATCAGGAACC
TGATCCGCGAGGACAAGGTGCACCAGATCTATTCCCAGATGCAGGTCGGCCAGGAGAAGTTCGGTATGCAGACCATGAAT
CAATGCCTGATGAACCTTTTGGCCAAGCGCATCATAACCGTTGAAGACGCCCTCGGGCGCTCCTCCGAGCCGGACGAACT
GAAGCAGATGCTCGCACCGGGTGCGGGTGCCGGACAGATGAGGCGCCCCCCCCAGAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B5EGW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

52.616

93.989

0.495
  pilT Legionella pneumophila strain ERS1305867

52.616

93.989

0.495
  pilT Acinetobacter baylyi ADP1

52.023

94.536

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.58

97.541

0.484
  pilT Pseudomonas aeruginosa PAK

50.867

94.536

0.481
  pilT Acinetobacter baumannii D1279779

50

94.536

0.473
  pilT Pseudomonas stutzeri DSM 10701

50

94.536

0.473
  pilT Acinetobacter baumannii strain A118

50

94.536

0.473
  pilT Acinetobacter nosocomialis M2

49.711

94.536

0.47
  pilT Vibrio cholerae strain A1552

47.463

91.53

0.434
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.463

91.53

0.434
  pilT Neisseria meningitidis 8013

46.108

91.257

0.421
  pilT Neisseria gonorrhoeae MS11

45.646

90.984

0.415
  pilU Vibrio cholerae strain A1552

42.254

96.995

0.41
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.721

0.407
  pilU Acinetobacter baylyi ADP1

41.667

95.082

0.396

Multiple sequence alignment