Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CDG61_RS04210 Genome accession   NZ_CP032143
Coordinates   775992..777029 (-) Length   345 a.a.
NCBI ID   WP_087513387.1    Uniprot ID   A0A3B7LSZ2
Organism   Acinetobacter sp. WCHAc010052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 770992..782029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG61_RS04180 (CDG61_04180) - 771740..772195 (+) 456 WP_087513381.1 bacteriohemerythrin -
  CDG61_RS04185 (CDG61_04185) - 772344..773429 (+) 1086 WP_087551287.1 hypothetical protein -
  CDG61_RS04190 (CDG61_04190) - 773426..773749 (+) 324 WP_087551286.1 RnfH family protein -
  CDG61_RS04195 (CDG61_04195) - 773795..774193 (-) 399 WP_087513384.1 outer membrane protein assembly factor BamE -
  CDG61_RS04200 (CDG61_04200) fur 774306..774743 (+) 438 WP_087513385.1 ferric iron uptake transcriptional regulator -
  CDG61_RS04205 (CDG61_04205) pilU 774848..775966 (-) 1119 WP_087513386.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDG61_RS04210 (CDG61_04210) pilT 775992..777029 (-) 1038 WP_087513387.1 type IV pilus twitching motility protein PilT Machinery gene
  CDG61_RS04215 (CDG61_04215) - 777152..777838 (+) 687 WP_087551285.1 YggS family pyridoxal phosphate-dependent enzyme -
  CDG61_RS04220 (CDG61_04220) - 778014..778817 (+) 804 WP_087551305.1 thioesterase family protein -
  CDG61_RS04225 (CDG61_04225) - 779027..779779 (+) 753 WP_087551304.1 hypothetical protein -
  CDG61_RS04230 (CDG61_04230) - 779842..780261 (+) 420 WP_087551284.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38368.99 Da        Isoelectric Point: 6.5777

>NTDB_id=313864 CDG61_RS04210 WP_087513387.1 775992..777029(-) (pilT) [Acinetobacter sp. WCHAc010052]
MDITELLAFSAKNGASDLHLSSGMPPLIRVDGEVRRINLPPLEHKEVHKLIYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGHIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQTARTMAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=313864 CDG61_RS04210 WP_087513387.1 775992..777029(-) (pilT) [Acinetobacter sp. WCHAc010052]
ATGGATATTACAGAATTACTGGCATTTTCTGCGAAAAACGGGGCTTCAGACTTACACCTGTCTTCAGGCATGCCGCCACT
TATTCGTGTGGATGGTGAAGTCCGCCGCATTAATCTGCCTCCCCTGGAACACAAAGAAGTTCACAAGCTGATTTATGACA
TCATGAATGATAAACAGCGTCGTGACTATGAAGAAAATCTTGAAACTGACTTTTCTTTTGAAGTTCCAGGCGTTGCCCGC
TTCCGTGTGAATGCATTTAACCAGAACCGTGGTGCAGGTGCTGTATTCCGTACCATTCCATCCAAAGTTCTGACGATGGA
AGATCTCGGTATGGGTCATATCTTCAAAGAAATCTGTGACTATCCGCGAGGTATTGTACTGGTGACTGGACCAACGGGTT
CGGGTAAATCCACCACACTGGCAGCAATGATTGATTATATTAACGATAACCGTTATGACCATATTCTGACGGTCGAAGAC
CCTATCGAATTTGTGCATCAGTCAAAAAAATGTCTGGTCAACCAGCGTGAAGTCCATCGTGATACTCATGGATTTAACGA
AGCATTACGTTCCGCACTACGTGAAGACCCTGACATTATCCTCGTCGGTGAGATGCGTGACCTTGAAACGATCCGTCTTG
CTTTGACCGCAGCTGAAACCGGTCACCTTGTTTTTGGTACTCTGCACACCACATCTGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGCTTTCAGAATCCTTACAGGCTGTAATTTCTCAGAC
CCTGTTGAAAAAGAATGGTGGTGGACGTGTGGCAGCTCATGAAATCATGATCGGTATTCCTGCGATCCGTAACTTAATCC
GTGAAAACAAAGTTGCACAGATGTATTCGGCCATTCAGACAGGTGCCAACTACGGTATGACCACACTGGATCAAAGCCTT
AAAACACTGGTCAGTAAAGGTGTTATCAGTCCTCAGACTGCCCGTACTATGGCGAAACAGCCTGAATCCTTCCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7LSZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.203

100

0.942
  pilT Acinetobacter baumannii strain A118

94.203

100

0.942
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.884

99.71

0.846
  pilT Pseudomonas stutzeri DSM 10701

84.593

99.71

0.843
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.452

97.391

0.667
  pilT Vibrio cholerae strain A1552

68.452

97.391

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment