Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CDG61_RS04205 Genome accession   NZ_CP032143
Coordinates   774848..775966 (-) Length   372 a.a.
NCBI ID   WP_087513386.1    Uniprot ID   A0A3B7LZI2
Organism   Acinetobacter sp. WCHAc010052     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769848..780966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG61_RS04175 (CDG61_04175) - 770639..771487 (+) 849 WP_087513440.1 class II glutamine amidotransferase -
  CDG61_RS04180 (CDG61_04180) - 771740..772195 (+) 456 WP_087513381.1 bacteriohemerythrin -
  CDG61_RS04185 (CDG61_04185) - 772344..773429 (+) 1086 WP_087551287.1 hypothetical protein -
  CDG61_RS04190 (CDG61_04190) - 773426..773749 (+) 324 WP_087551286.1 RnfH family protein -
  CDG61_RS04195 (CDG61_04195) - 773795..774193 (-) 399 WP_087513384.1 outer membrane protein assembly factor BamE -
  CDG61_RS04200 (CDG61_04200) fur 774306..774743 (+) 438 WP_087513385.1 ferric iron uptake transcriptional regulator -
  CDG61_RS04205 (CDG61_04205) pilU 774848..775966 (-) 1119 WP_087513386.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDG61_RS04210 (CDG61_04210) pilT 775992..777029 (-) 1038 WP_087513387.1 type IV pilus twitching motility protein PilT Machinery gene
  CDG61_RS04215 (CDG61_04215) - 777152..777838 (+) 687 WP_087551285.1 YggS family pyridoxal phosphate-dependent enzyme -
  CDG61_RS04220 (CDG61_04220) - 778014..778817 (+) 804 WP_087551305.1 thioesterase family protein -
  CDG61_RS04225 (CDG61_04225) - 779027..779779 (+) 753 WP_087551304.1 hypothetical protein -
  CDG61_RS04230 (CDG61_04230) - 779842..780261 (+) 420 WP_087551284.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41806.00 Da        Isoelectric Point: 6.5062

>NTDB_id=313863 CDG61_RS04205 WP_087513386.1 774848..775966(-) (pilU) [Acinetobacter sp. WCHAc010052]
MDFNDLLNLMIQQKASDLFITADVEPSMKINGQIVPVANTKLTGDIVGQLLNSIMNDKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDQPGMVLRRIETTIPTMDDLKLPPILKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNHNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGADRLMSAGSQITYDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=313863 CDG61_RS04205 WP_087513386.1 774848..775966(-) (pilU) [Acinetobacter sp. WCHAc010052]
ATGGATTTTAATGATTTACTGAACCTGATGATTCAGCAGAAAGCTTCGGATCTGTTCATTACAGCAGATGTTGAACCTTC
AATGAAAATTAATGGTCAGATTGTGCCTGTAGCAAACACAAAACTGACTGGAGATATTGTTGGGCAGCTGCTCAACAGTA
TTATGAATGATAAACAGCGTAAAGAATTTGCAGATACCCGCGAATGTAACTTTGCCATCATGAACCGTGATAAAACTGCA
CGTTTCCGTGTCAGTGCATTTCAGCAACGTGACCAGCCTGGCATGGTTTTACGTCGGATTGAAACCACGATTCCAACCAT
GGATGACCTAAAACTTCCACCGATCTTAAAAGAACTTGCCATGACCAAACGTGGCATCATCATTTTCGTTGGTGCGACAG
GTACAGGTAAATCCACATCACTGGCTTCCATCATTGGATACCGAAATCATAATTCAAAGGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTTCATGAACATGCTGGCTGTATCATTACGCAGCGTGAAGTCGGTATTGATACAGATTCTTTTGA
AATTGCACTGAAAAATACACTGCGTCAGGCACCTGATGTGATTCTGATCGGGGAGATCCGTTCCCGCGAAACAATGGACT
ATGCCATTGCATTCGCAGAAACCGGTCACCTGGTATTTGCTACACTGCACGCCAACAACGCCAACCAGGCGATTGACCGT
ATCATTCACTTCTTCGAAGCAGACCGTCACAGCCAGCTGTTCATGGATCTGTCCCTGAACTTAAAAGCTATGGTTGCACA
GCAGCTGATTCCAACACCGGACGGTAACTCCAGACGTGCTGCTATTGAGATTCTGATTAACTCACCTTTAATTTCTGACT
TAATCCGTAAAGGTGAAATTCATGAAATTAAAGATCTGATGAAACGTTCACGTGAACTGGGAATGCAGACGTTTGACCAG
GCCTTATATGACCTGTATAAAGCCAAGCAGATTACCTATAAAGATGCACTGAAACATGCAGACTCACCAAATGACCTGCG
CTTACAGATCAAACTTTCTGAAGAAGGTGCTGACCGCCTGATGAGTGCAGGTTCTCAGATTACCTATGACGGACAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7LZI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.731

0.68
  pilU Vibrio cholerae strain A1552

56.695

94.355

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.775

93.011

0.398
  pilT Legionella pneumophila strain ERS1305867

42.899

90.86

0.39
  pilT Legionella pneumophila strain Lp02

42.899

90.86

0.39
  pilT Acinetobacter baumannii D1279779

40.233

92.204

0.371
  pilT Acinetobacter nosocomialis M2

40.233

92.204

0.371
  pilT Acinetobacter baumannii strain A118

40.233

92.204

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.031

94.355

0.368
  pilT Vibrio cholerae strain A1552

39.031

94.355

0.368
  pilT Pseudomonas aeruginosa PAK

39.595

93.011

0.368
  pilT Neisseria gonorrhoeae MS11

39.595

93.011

0.368
  pilT Neisseria meningitidis 8013

39.595

93.011

0.368
  pilT Acinetobacter baylyi ADP1

39.65

92.204

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.306

93.011

0.366

Multiple sequence alignment