Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Ahae5227_RS12305 Genome accession   NZ_CP031988
Coordinates   2566186..2567223 (+) Length   345 a.a.
NCBI ID   WP_004638216.1    Uniprot ID   N9GFN0
Organism   Acinetobacter haemolyticus strain 5227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2561186..2572223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ahae5227_RS12290 (Ahae5227_12445) - 2564330..2564617 (+) 288 WP_008940859.1 hypothetical protein -
  Ahae5227_RS12295 (Ahae5227_12450) - 2564689..2565360 (+) 672 WP_008940858.1 HAD family hydrolase -
  Ahae5227_RS12300 (Ahae5227_12455) - 2565367..2566059 (-) 693 WP_005091345.1 YggS family pyridoxal phosphate-dependent enzyme -
  Ahae5227_RS12305 (Ahae5227_12460) pilT 2566186..2567223 (+) 1038 WP_004638216.1 type IV pilus twitching motility protein PilT Machinery gene
  Ahae5227_RS12310 (Ahae5227_12465) pilU 2567252..2568370 (+) 1119 WP_005081584.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Ahae5227_RS12315 (Ahae5227_12470) fur 2568458..2568895 (-) 438 WP_004638213.1 ferric iron uptake transcriptional regulator -
  Ahae5227_RS12320 (Ahae5227_12475) - 2569007..2569405 (+) 399 WP_004638212.1 outer membrane protein assembly factor BamE -
  Ahae5227_RS12325 (Ahae5227_12480) - 2569462..2569782 (-) 321 WP_005091348.1 RnfH family protein -
  Ahae5227_RS12330 (Ahae5227_12485) - 2569782..2570864 (-) 1083 WP_005091350.1 membrane protein -
  Ahae5227_RS12335 (Ahae5227_12490) - 2571114..2571569 (-) 456 WP_008940857.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.96 Da        Isoelectric Point: 6.3479

>NTDB_id=313177 Ahae5227_RS12305 WP_004638216.1 2566186..2567223(+) (pilT) [Acinetobacter haemolyticus strain 5227]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGTIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KSLVSKGLISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=313177 Ahae5227_RS12305 WP_004638216.1 2566186..2567223(+) (pilT) [Acinetobacter haemolyticus strain 5227]
ATGGATATCACAGAATTACTCGCCTTTTCAGTAAAAAATGGTGCATCGGATTTACACCTTTCGGCGGGAATGCCACCTAT
GATTCGTGTAGATGGTGAGGTTCGCCGAATTAATCTACCAGCTCTAGAGCATAAAGATGTCCATCGTCTTGTTTATGACA
TCATGAACGATAAGCAACGTCGTGATTTTGAGGAAGATCTTGAAACTGACTTCTCTTTTGAAGTACCCAATATCGCACGT
TTCCGTGTCAACGCATTCAACCAAAACCGTGGTGCGGGTGCCGTATTCCGTACCATTCCATCCAAAGTTTTAACCATGGA
AGACTTGGGTTTAGGCACAATTTTCAAAGAAATTTGTGATTATCCGCGAGGAATCGTATTGGTCACTGGACCTACAGGTT
CGGGTAAATCGACAACTCTCGCAGCAATGATTGATTACATCAATGAAAATCGTTACGACCATATCCTAACGGTTGAAGAT
CCAATCGAATTTGTACACCAATCAAAAAAATGCCTGATTAACCAACGTGAAGTGCATCGTGATACCCATGGTTTTAACGA
AGCATTACGTTCTGCACTGCGTGAAGACCCTGACATTATTCTGGTCGGTGAGATGCGTGACTTAGAGACCATTCGCCTTG
CACTCACCGCAGCGGAAACAGGTCACCTTGTATTTGGTACACTGCATACCACCTCAGCAGCAAAAACCATTGACCGTGTG
ATCGACGTATTCCCAGCCGAGGAAAAAGACATGGTTCGTGCCATGCTCTCAGAATCATTACAAGCGGTAATTTCTCAAAC
CTTATTGAAAAAGAATGGCGGCGGTCGTGTGGCAGCACATGAAATCATGATCGGGATTCCTGCGATCCGTAACCTCGTCC
GTGAAAACAAAGTCGCACAAATGTATTCTGCGATCCAAACAGGAGCGAACTATGGCATGACGACTTTAGATCAAAGTCTA
AAAAGTCTGGTATCTAAAGGTCTTATTAGTCCACAAACCGCACGTACAGTTGCAAAGCAACCAGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9GFN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.522

100

0.965
  pilT Acinetobacter baumannii D1279779

96.232

100

0.962
  pilT Acinetobacter baumannii strain A118

96.232

100

0.962
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394

Multiple sequence alignment