Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Ahae5227_RS12310 Genome accession   NZ_CP031988
Coordinates   2567252..2568370 (+) Length   372 a.a.
NCBI ID   WP_005081584.1    Uniprot ID   N9GMD9
Organism   Acinetobacter haemolyticus strain 5227     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2562252..2573370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ahae5227_RS12290 (Ahae5227_12445) - 2564330..2564617 (+) 288 WP_008940859.1 hypothetical protein -
  Ahae5227_RS12295 (Ahae5227_12450) - 2564689..2565360 (+) 672 WP_008940858.1 HAD family hydrolase -
  Ahae5227_RS12300 (Ahae5227_12455) - 2565367..2566059 (-) 693 WP_005091345.1 YggS family pyridoxal phosphate-dependent enzyme -
  Ahae5227_RS12305 (Ahae5227_12460) pilT 2566186..2567223 (+) 1038 WP_004638216.1 type IV pilus twitching motility protein PilT Machinery gene
  Ahae5227_RS12310 (Ahae5227_12465) pilU 2567252..2568370 (+) 1119 WP_005081584.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Ahae5227_RS12315 (Ahae5227_12470) fur 2568458..2568895 (-) 438 WP_004638213.1 ferric iron uptake transcriptional regulator -
  Ahae5227_RS12320 (Ahae5227_12475) - 2569007..2569405 (+) 399 WP_004638212.1 outer membrane protein assembly factor BamE -
  Ahae5227_RS12325 (Ahae5227_12480) - 2569462..2569782 (-) 321 WP_005091348.1 RnfH family protein -
  Ahae5227_RS12330 (Ahae5227_12485) - 2569782..2570864 (-) 1083 WP_005091350.1 membrane protein -
  Ahae5227_RS12335 (Ahae5227_12490) - 2571114..2571569 (-) 456 WP_008940857.1 bacteriohemerythrin -
  Ahae5227_RS12340 (Ahae5227_12495) - 2571844..2572692 (-) 849 WP_017395122.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41758.89 Da        Isoelectric Point: 6.4343

>NTDB_id=313178 Ahae5227_RS12310 WP_005081584.1 2567252..2568370(+) (pilU) [Acinetobacter haemolyticus strain 5227]
MDFNDLLNLMIQQNASDLFITADVEPSMKINGQIVPVSKTKLSAEIVGQLVNSIMSDKQRKEFADTRECNFAITNREKTA
RFRVSAFQQRDMPGMILRKIETHIPSMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNHNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLNASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=313178 Ahae5227_RS12310 WP_005081584.1 2567252..2568370(+) (pilU) [Acinetobacter haemolyticus strain 5227]
ATGGATTTTAATGATTTACTCAATTTGATGATTCAACAAAACGCATCTGATTTATTTATCACTGCGGATGTTGAACCATC
TATGAAAATTAACGGGCAAATTGTGCCTGTTTCTAAAACCAAATTATCTGCTGAAATTGTGGGGCAACTTGTCAATTCAA
TTATGAGTGACAAGCAACGTAAAGAATTTGCAGATACACGTGAGTGTAACTTCGCAATTACTAACCGTGAAAAAACAGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGCGATATGCCTGGGATGATTTTACGTAAAATCGAAACTCATATTCCGAGTAT
GGACGATTTAAAACTGCCTGAAGTACTCAAAGAACTTGCCATGACCAAACGTGGAATTATTATCTTTGTTGGCGCAACAG
GTACAGGTAAATCTACTTCCCTCGCTTCAATGATTGGTTATCGTAATCACAATTCCAAAGGACACATTATCACCATCGAA
GATCCGATTGAATTTGTGCATCAACATGCGGGGTGTATCGTCACGCAACGTGAAGTCGGGATTGATACTGAATCTTTTGA
AGTTGCATTAAAAAATACACTTCGTCAGGCACCAGATGTTATTTTGATTGGTGAGATTCGTTCACGCGAAACCATGGACT
ATGCGATCGCCTTTGCTGAAACAGGTCACTTGGTCTTGGCAACGCTTCACGCCAACAATGCCAACCAAGCCCTAGATCGT
ATTATTCACTTCTTCGAAGCCGATCGTCATAGCCAGTTGTATATGGATCTGTCACTTAATTTAAAGGCATTGGTCGCGCA
ACAATTGATCCCAACACCTGATGGAAATTCACGTCGTGCTGCGATTGAAATTTTGATCAATACTCCTTTATTGGCTGACT
ATATTCGTAAAGGTGAAATTCATGAGATTAAAGATCTGATGAAACGCTCTCGTGAATTGGGTATGCAAACCTTTGACCAA
GCCCTATTTGACCTCTATAAAGCGGGACAAATCACTTACAAAGATGCATTAAAGCACGCAGACTCACCAAACGACTTGCG
TCTCACCATTAAGCTTTCTGAAGAAGGCGCAGATCAACTACTGAATGCGAGCCGTAACATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9GMD9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

70.081

99.731

0.699
  pilU Vibrio cholerae strain A1552

54.324

99.462

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.385

97.849

0.395
  pilT Legionella pneumophila strain Lp02

41.42

90.86

0.376
  pilT Legionella pneumophila strain ERS1305867

41.42

90.86

0.376
  pilT Neisseria meningitidis 8013

40.294

91.398

0.368
  pilT Neisseria gonorrhoeae MS11

40.294

91.398

0.368
  pilT Pseudomonas aeruginosa PAK

39.017

93.011

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36

Multiple sequence alignment