Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D1814_RS12505 Genome accession   NZ_CP031967
Coordinates   2892537..2893580 (+) Length   347 a.a.
NCBI ID   WP_118492739.1    Uniprot ID   A0A3A9UGV4
Organism   Alteromonas sp. BL110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2887537..2898580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1814_RS12485 (D1814_12485) - 2889821..2890270 (-) 450 WP_118495382.1 DUF4426 domain-containing protein -
  D1814_RS12490 (D1814_12490) - 2890382..2890918 (-) 537 WP_118492733.1 YggT family protein -
  D1814_RS12495 (D1814_12495) proC 2890939..2891760 (-) 822 WP_118492735.1 pyrroline-5-carboxylate reductase -
  D1814_RS12500 (D1814_12500) - 2891823..2892509 (-) 687 WP_118492737.1 YggS family pyridoxal phosphate-dependent enzyme -
  D1814_RS12505 (D1814_12505) pilT 2892537..2893580 (+) 1044 WP_118492739.1 type IV pilus twitching motility protein PilT Machinery gene
  D1814_RS12510 (D1814_12510) pilU 2893589..2894701 (+) 1113 WP_118492741.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D1814_RS12515 (D1814_12515) - 2894835..2895152 (+) 318 WP_118492743.1 DUF2007 domain-containing protein -
  D1814_RS12520 (D1814_12520) ruvX 2895199..2895630 (-) 432 WP_118492745.1 Holliday junction resolvase RuvX -
  D1814_RS12525 (D1814_12525) - 2895623..2896189 (-) 567 WP_118492747.1 YqgE/AlgH family protein -
  D1814_RS12530 (D1814_12530) gshB 2896329..2897282 (-) 954 WP_118492749.1 glutathione synthase -
  D1814_RS12535 (D1814_12535) - 2897421..2898149 (-) 729 WP_118492751.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38424.16 Da        Isoelectric Point: 6.4425

>NTDB_id=312941 D1814_RS12505 WP_118492739.1 2892537..2893580(+) (pilT) [Alteromonas sp. BL110]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHGLVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKDIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHENKLCLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVISKEDAAAKSVDKQSMNNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=312941 D1814_RS12505 WP_118492739.1 2892537..2893580(+) (pilT) [Alteromonas sp. BL110]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCGGGCCTCCCACCAAT
TATTCGTGTAGATGGCGAGATGCGTAAACTTAACGTGCCTGCCCTCGATCACAAGCAAGTTCATGGTCTTGTTTACGAGA
TTATGAATGACATGCAGCGCAAAGAGTACGAAGAAAACTTAGAAACCGATTTTTCATTCGAAGTAAACGGTCTGTCTCGT
TTTCGTGTTAATGCCTTTGTTCAAAATCGCGGGGCTGCGGCGGTATTGCGTACTATTCCTAGCAAGGTGCTGACCTTAGA
CGACCTTGGCGCCCCCGAAATTTTTAAAGACATCATTAATCAGCCTACCGGAATAGTGCTAGTCACTGGCGCTACAGGCT
CGGGTAAAAGTACCACCCTCGCGGCTATGGTTGACCATATCAACACGCACAAACGCGAACATATTCTTACCATTGAAGAT
CCCATTGAATTTGTCCACGAGAATAAGCTCTGCTTAGTAAACCAGCGTGAAGTACACCGCGATACACACTCGTTTAACAA
CGCCCTGCGCTCAGCGCTTCGTGAAGACCCCGATGTAATTTTGGTGGGTGAGCTTCGTGACTTGGAAACCATTCGCCTTG
CTATTTCAGCTGCAGAAACAGGCCACTTGGTGTTTGGTACACTACACACCAATTCAGCACCGAAAACCATTGACCGTATC
ATTGATGTGTTCCCAGCAGAGGAAAAAGCCATGGTACGTTCGATGTTATCGGAGTCGCTTCGCGCGGTTATATCGCAAAC
CCTGCTTAAGAAAGTAGGAGGAGGCCGAATTGCAGCCCATGAAATAATGGTGGGCATCCCAGCTATACGAAACCTTATTC
GTGAAGATAAAGTGCCGCAGATGTACTCGGTTATTCAAACCGGGCAAGCGCATGGTATGCAAACCATGGATCAGTGCTTA
CAGAGATTGGTAGCTATGGGGGTCATTAGCAAAGAAGATGCGGCGGCAAAGTCAGTAGATAAACAGTCTATGAACAACTT
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A9UGV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.163

99.135

0.755
  pilT Acinetobacter baumannii strain A118

76.163

99.135

0.755
  pilT Acinetobacter nosocomialis M2

75.872

99.135

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.419

99.135

0.738
  pilT Pseudomonas aeruginosa PAK

75.74

97.406

0.738
  pilT Legionella pneumophila strain Lp02

71.848

98.271

0.706
  pilT Legionella pneumophila strain ERS1305867

71.848

98.271

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.118

0.703
  pilT Vibrio cholerae strain A1552

72.404

97.118

0.703
  pilT Neisseria meningitidis 8013

64.74

99.712

0.646
  pilT Neisseria gonorrhoeae MS11

64.451

99.712

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.312

98.847

0.507
  pilU Acinetobacter baylyi ADP1

42.012

97.406

0.409
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406

Multiple sequence alignment