Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D1814_RS12510 Genome accession   NZ_CP031967
Coordinates   2893589..2894701 (+) Length   370 a.a.
NCBI ID   WP_118492741.1    Uniprot ID   -
Organism   Alteromonas sp. BL110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2888589..2899701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1814_RS12485 (D1814_12485) - 2889821..2890270 (-) 450 WP_118495382.1 DUF4426 domain-containing protein -
  D1814_RS12490 (D1814_12490) - 2890382..2890918 (-) 537 WP_118492733.1 YggT family protein -
  D1814_RS12495 (D1814_12495) proC 2890939..2891760 (-) 822 WP_118492735.1 pyrroline-5-carboxylate reductase -
  D1814_RS12500 (D1814_12500) - 2891823..2892509 (-) 687 WP_118492737.1 YggS family pyridoxal phosphate-dependent enzyme -
  D1814_RS12505 (D1814_12505) pilT 2892537..2893580 (+) 1044 WP_118492739.1 type IV pilus twitching motility protein PilT Machinery gene
  D1814_RS12510 (D1814_12510) pilU 2893589..2894701 (+) 1113 WP_118492741.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D1814_RS12515 (D1814_12515) - 2894835..2895152 (+) 318 WP_118492743.1 DUF2007 domain-containing protein -
  D1814_RS12520 (D1814_12520) ruvX 2895199..2895630 (-) 432 WP_118492745.1 Holliday junction resolvase RuvX -
  D1814_RS12525 (D1814_12525) - 2895623..2896189 (-) 567 WP_118492747.1 YqgE/AlgH family protein -
  D1814_RS12530 (D1814_12530) gshB 2896329..2897282 (-) 954 WP_118492749.1 glutathione synthase -
  D1814_RS12535 (D1814_12535) - 2897421..2898149 (-) 729 WP_118492751.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40912.82 Da        Isoelectric Point: 5.9000

>NTDB_id=312942 D1814_RS12510 WP_118492741.1 2893589..2894701(+) (pilU) [Alteromonas sp. BL110]
MLDSLLQKMVDTNASDLFITAEFPVSAKINGKLTPVTESATSVEESLALVHEAMTEKQRDEFHTSKECNFAIVRDGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=312942 D1814_RS12510 WP_118492741.1 2893589..2894701(+) (pilU) [Alteromonas sp. BL110]
ATGCTAGATTCACTATTACAAAAAATGGTCGATACCAATGCGTCGGATTTATTTATAACGGCTGAGTTTCCGGTAAGCGC
CAAAATTAACGGTAAACTAACGCCAGTGACGGAAAGTGCTACGTCGGTAGAAGAGTCGCTCGCGCTGGTTCACGAAGCGA
TGACCGAAAAGCAGCGAGACGAATTTCATACGTCGAAGGAATGTAATTTCGCCATCGTACGTGACGGTATTGGCCGTTTC
CGTTGTTCAGCATTCTGGCAGCGCGACCAAGCGGGTATGGTTATCCGTCGAATCGTGACACAAATTCCTAAAGCTGATGA
TTTGGGTCTGCCGCGAGTACTAAAAGAAATTATTATGTCTAAACGCGGTCTTGTTCTATTTGTAGGTGGAACAGGTACAG
GTAAATCAACGTCACTGGCTGCGCTCATTGGGCATAGAAATGAGAATTCTCATGGGCATATATTAACCATTGAGGATCCT
ATCGAATTTGTCCACGAGCATAAGAATTGCGTAATTACTCAGCGAGAGGTGGGTATCGATACCCAGTCTTTCGATGACGC
ACTTAAAAGCTCGCTTCGTCAGGCCCCTGATGTCATTTTGATTGGTGAAATCCGTTCAATGGAAACCATGGAATATGCCA
TGTCGTTTGCCGATACAGGGCATTTATGTGTGGCAACGCTACACGCCAATAACGCGAACCAAGCTATAGAGCGCATTATG
CACTTGGCGCCTAAAGATCAGCACGATAAGCTGCGCTTCGATTTAAGCCTGAACATACGCGCTATTGTTGCTCAACAACT
TGTGCCGACACCAGACGGTAAGGGCAGGGTAGCGGCTATAGAAATACTGCTTAATTCGCCGTTAGTGCGCGACCTTATTC
AGCGCAACGAAATAGGCGCATTAAAAGAAGCTATGAAAAAGGGTAAAGAGCAGGGCATGCAAAGCTTTGATATGGCTCTT
TATGACCTTTATAAAGCGGGCAAGATTGATATGGACCAGGCGCTTCACCATGCTGACTCGCCTAACGATCTGCGCTTAAT
GATCAAGCTTGATACCAGTGACGGCTCTAGCTTAGGCACCTTGTCTAATGTGTCTATTGATATGGATGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Vibrio cholerae strain A1552

56.522

99.459

0.562
  pilU Acinetobacter baylyi ADP1

59.143

94.595

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.765

91.892

0.384
  pilT Acinetobacter nosocomialis M2

39.412

91.892

0.362
  pilT Acinetobacter baumannii D1279779

39.412

91.892

0.362
  pilT Acinetobacter baumannii strain A118

39.412

91.892

0.362

Multiple sequence alignment