Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HYN24_RS14495 Genome accession   NZ_CP031842
Coordinates   3015077..3016120 (-) Length   347 a.a.
NCBI ID   WP_117609918.1    Uniprot ID   A0A3B7A8C6
Organism   Dechloromonas sp. HYN0024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3010077..3021120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYN24_RS14470 (HYN24_14470) - 3010226..3010591 (-) 366 WP_117609914.1 hypothetical protein -
  HYN24_RS14475 (HYN24_14475) - 3010731..3012722 (-) 1992 WP_117609915.1 UvrD-helicase domain-containing protein -
  HYN24_RS14480 (HYN24_14480) - 3012841..3013158 (-) 318 WP_117609916.1 hypothetical protein -
  HYN24_RS14485 (HYN24_14485) - 3013209..3013895 (-) 687 WP_205421400.1 DUF4197 domain-containing protein -
  HYN24_RS14490 (HYN24_14490) pilU 3013912..3015048 (-) 1137 WP_117609917.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HYN24_RS14495 (HYN24_14495) pilT 3015077..3016120 (-) 1044 WP_117609918.1 type IV pilus twitching motility protein PilT Machinery gene
  HYN24_RS14500 (HYN24_14500) - 3016177..3016866 (+) 690 WP_117609919.1 YggS family pyridoxal phosphate-dependent enzyme -
  HYN24_RS14505 (HYN24_14505) proC 3016863..3017675 (+) 813 WP_117609920.1 pyrroline-5-carboxylate reductase -
  HYN24_RS14510 (HYN24_14510) - 3017675..3018208 (+) 534 WP_117609921.1 YggT family protein -
  HYN24_RS14515 (HYN24_14515) - 3018205..3018501 (+) 297 WP_117609922.1 DUF167 domain-containing protein -
  HYN24_RS14525 (HYN24_14525) - 3018786..3019469 (-) 684 WP_117609924.1 SDR family NAD(P)-dependent oxidoreductase -
  HYN24_RS14530 (HYN24_14530) - 3019723..3020031 (+) 309 WP_117609925.1 NIPSNAP family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38385.97 Da        Isoelectric Point: 6.7807

>NTDB_id=311775 HYN24_RS14495 WP_117609918.1 3015077..3016120(-) (pilT) [Dechloromonas sp. HYN0024]
MDITELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPAMEHKDVHGMVYDIMNDGQRKIYEETLECDFSFEIPNLAR
FRVNAFNQHRGAGAVFRTIPSKVLTLEELNAPKIFQEISEYPRGIVLVTGPTGSGKSTTLAGMVNHVNENDYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNFGMQTLDQN
LIDLVRRNVVSSAEAKFKAANKDAFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=311775 HYN24_RS14495 WP_117609918.1 3015077..3016120(-) (pilT) [Dechloromonas sp. HYN0024]
ATGGACATCACCGAACTGCTGGCTTTCAGCGTCAAGAACAAGGCCTCCGATCTCCACCTGTCATCCGGCCTGCCGCCGAT
GATCCGTGTGCATGGCGACGTCCGGCGCATCAACCTGCCGGCCATGGAACACAAGGATGTTCATGGCATGGTCTATGACA
TCATGAACGATGGCCAGCGCAAGATTTATGAAGAGACGCTGGAATGCGACTTCTCGTTTGAAATCCCCAACCTGGCCCGT
TTCCGGGTTAATGCCTTCAATCAGCACCGTGGTGCTGGCGCCGTGTTCCGGACCATTCCGTCCAAAGTGCTGACGCTCGA
GGAATTGAACGCGCCGAAGATTTTCCAGGAAATTTCCGAGTATCCGCGCGGCATCGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACGACGCTGGCCGGCATGGTCAATCACGTAAACGAAAACGATTACGGCCATATCCTGACCGTCGAAGAC
CCGATCGAATTCGTCCATGAATCGAAAAAGTGCCTGATCAACCAGCGCGAAGTCGGGCCGCATACCCTGTCCTTCTCGAA
CGCTCTGCGTTCGGCCCTGCGTGAGGATCCGGACGTCATCCTGGTCGGTGAAATGCGTGACCTGGAAACCATCCGTCTGG
CACTGACCGCAGCCGAAACCGGCCACCTCGTGTTCGGCACGCTGCACACCTCTTCAGCCGCCAAGACGGTGGACCGTATC
GTTGACGTTTTCCCGGCGGCGGAAAAGGAGATGGTTCGTTCGATGCTCTCTGAGTCGCTGCGCGCCGTTATCTCGCAGAC
GCTGCTCAAGACCAAGGACGGCAGCGGCCGCGTCGCCGCGCACGAAATCATGATCGGCACCCCGGCCATCCGCAACCTCA
TTCGCGAGAACAAGGTGGCCCAGATGTATTCGGCAATCCAGACCGGTCAGAACTTCGGCATGCAGACGCTGGACCAGAAC
CTCATCGATCTGGTGCGACGCAATGTGGTGTCGAGTGCCGAAGCGAAATTCAAGGCAGCCAACAAGGACGCCTTCCCGGC
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7A8C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.551

99.424

0.781
  pilT Acinetobacter baumannii strain A118

78.551

99.424

0.781
  pilT Acinetobacter nosocomialis M2

78.551

99.424

0.781
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Neisseria meningitidis 8013

73.043

99.424

0.726
  pilT Neisseria gonorrhoeae MS11

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

71.884

99.424

0.715
  pilT Legionella pneumophila strain ERS1305867

71.884

99.424

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.118

97.406

0.683
  pilT Vibrio cholerae strain A1552

70.118

97.406

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.525

98.847

0.401
  pilU Acinetobacter baylyi ADP1

41.369

96.83

0.401

Multiple sequence alignment