Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HYN24_RS14490 Genome accession   NZ_CP031842
Coordinates   3013912..3015048 (-) Length   378 a.a.
NCBI ID   WP_117609917.1    Uniprot ID   A0A3B7A9X9
Organism   Dechloromonas sp. HYN0024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3008912..3020048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYN24_RS14460 (HYN24_14460) - 3009159..3009341 (+) 183 WP_117609912.1 hypothetical protein -
  HYN24_RS14465 (HYN24_14465) - 3009655..3010101 (+) 447 WP_117609913.1 cytochrome c5 family protein -
  HYN24_RS14470 (HYN24_14470) - 3010226..3010591 (-) 366 WP_117609914.1 hypothetical protein -
  HYN24_RS14475 (HYN24_14475) - 3010731..3012722 (-) 1992 WP_117609915.1 UvrD-helicase domain-containing protein -
  HYN24_RS14480 (HYN24_14480) - 3012841..3013158 (-) 318 WP_117609916.1 hypothetical protein -
  HYN24_RS14485 (HYN24_14485) - 3013209..3013895 (-) 687 WP_205421400.1 DUF4197 domain-containing protein -
  HYN24_RS14490 (HYN24_14490) pilU 3013912..3015048 (-) 1137 WP_117609917.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HYN24_RS14495 (HYN24_14495) pilT 3015077..3016120 (-) 1044 WP_117609918.1 type IV pilus twitching motility protein PilT Machinery gene
  HYN24_RS14500 (HYN24_14500) - 3016177..3016866 (+) 690 WP_117609919.1 YggS family pyridoxal phosphate-dependent enzyme -
  HYN24_RS14505 (HYN24_14505) proC 3016863..3017675 (+) 813 WP_117609920.1 pyrroline-5-carboxylate reductase -
  HYN24_RS14510 (HYN24_14510) - 3017675..3018208 (+) 534 WP_117609921.1 YggT family protein -
  HYN24_RS14515 (HYN24_14515) - 3018205..3018501 (+) 297 WP_117609922.1 DUF167 domain-containing protein -
  HYN24_RS14525 (HYN24_14525) - 3018786..3019469 (-) 684 WP_117609924.1 SDR family NAD(P)-dependent oxidoreductase -
  HYN24_RS14530 (HYN24_14530) - 3019723..3020031 (+) 309 WP_117609925.1 NIPSNAP family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42258.76 Da        Isoelectric Point: 6.5906

>NTDB_id=311774 HYN24_RS14490 WP_117609917.1 3013912..3015048(-) (pilU) [Dechloromonas sp. HYN0024]
MERDQAMKFMHDLLRLMSQKKGSDLFITAGFPPAIKIDGKVTPVSNQVLTPQHTAELARALMNDRQAAEFEATKECNFAI
SPAGIGRFRVNTLVQQGRVGVVCRTINMTIPTLDELLLPPVLKDLAMTKRGLIIFVGGTGTGKTTSLAALVDYRNENSYG
HIITIEDPIEYVHEHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRNTMDYAIAFAETGHLCLATLHANSA
NQAIDRIVNFFPEERRQQLLMDLSLNLRAMVSQRLIPKKDGKGRMAAIEVLLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKITYEDALRNADSLNDLRLQIKLYGKESKDRDLSTGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=311774 HYN24_RS14490 WP_117609917.1 3013912..3015048(-) (pilU) [Dechloromonas sp. HYN0024]
ATGGAACGCGATCAGGCAATGAAATTCATGCACGATCTTCTGCGCCTGATGTCGCAGAAGAAAGGCTCGGACCTCTTTAT
CACCGCCGGATTTCCCCCGGCCATCAAAATCGATGGCAAGGTGACACCGGTTTCCAATCAGGTGCTGACGCCGCAACACA
CTGCCGAATTGGCGCGGGCGCTGATGAACGACCGGCAGGCCGCCGAGTTCGAGGCGACCAAGGAATGCAACTTCGCCATT
TCGCCGGCCGGTATTGGCCGTTTCCGCGTCAATACGCTGGTCCAGCAGGGGCGCGTCGGGGTTGTCTGCCGCACCATCAA
CATGACCATCCCGACCCTCGATGAACTGCTACTGCCTCCGGTGCTCAAGGATCTGGCGATGACCAAGCGTGGCCTGATCA
TCTTCGTCGGTGGCACCGGTACTGGCAAGACGACCTCGCTGGCTGCCCTGGTCGATTACCGCAACGAAAATTCCTACGGG
CACATCATCACCATCGAAGACCCGATCGAATACGTGCACGAGCACAAGAACTGTATCGTCACGCAGCGCGAAGTCGGCGT
TGATACCGATGACTGGGGCCCGGCCCTCAAGAACACCCTGCGTCAGGCACCGGATGTCATCCTGATGGGCGAAATCCGCG
ACCGCAACACCATGGATTACGCCATCGCCTTTGCCGAAACCGGCCATCTCTGTCTGGCGACGTTGCATGCCAACAGTGCC
AACCAGGCCATTGACCGGATCGTCAACTTCTTCCCGGAAGAGCGTCGCCAGCAATTGCTCATGGACCTCTCCCTCAATCT
GCGTGCCATGGTGTCGCAGCGCCTGATTCCGAAGAAGGATGGCAAGGGCCGCATGGCCGCCATCGAAGTGCTGCTCAATT
CGCCGCTGATTTCGGACCTCATCTTCAAGGGCGAGGTGCACGAGATCAAGGAGATCATGAAGAAATCGCGCGAACTCGGT
ATGCAGACCTTCGACCAGGCGCTCTTCGACCTCTACGAGGCAGGCAAGATCACCTACGAAGATGCCTTGCGCAATGCCGA
CTCGCTGAATGACCTGCGCCTGCAGATCAAGCTCTATGGCAAGGAATCGAAGGACCGGGATCTGTCGACCGGCATCGGTC
ACCTCGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7A9X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.011

96.296

0.616
  pilU Acinetobacter baylyi ADP1

59.61

94.974

0.566
  pilU Vibrio cholerae strain A1552

55.132

90.212

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas aeruginosa PAK

42.388

88.624

0.376
  pilT Acinetobacter baylyi ADP1

41.246

89.153

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.896

88.624

0.362

Multiple sequence alignment