Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CJA_RS00425 Genome accession   NC_010995
Coordinates   132115..133149 (-) Length   344 a.a.
NCBI ID   WP_012485770.1    Uniprot ID   B3PFH1
Organism   Cellvibrio japonicus Ueda107     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 88946..132093 132115..133149 flank 22


Gene organization within MGE regions


Location: 88946..133149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJA_RS00235 (CJA_0047) - 88946..89320 (+) 375 WP_012485731.1 Na+/H+ antiporter subunit C -
  CJA_RS00240 (CJA_0048) - 89317..90825 (+) 1509 WP_012485732.1 monovalent cation/H+ antiporter subunit D -
  CJA_RS00245 (CJA_0049) - 90859..91404 (+) 546 WP_012485733.1 Na+/H+ antiporter subunit E -
  CJA_RS00250 (CJA_0050) - 91386..91655 (+) 270 WP_012485734.1 K+/H+ antiporter subunit F -
  CJA_RS00255 (CJA_0051) - 91665..91994 (+) 330 WP_012485735.1 Na+/H+ antiporter subunit G -
  CJA_RS00260 (CJA_0052) - 92074..94611 (-) 2538 WP_012485736.1 heavy metal translocating P-type ATPase -
  CJA_RS00265 (CJA_0053) - 95000..97042 (+) 2043 WP_012485737.1 pectate lyase -
  CJA_RS00270 (CJA_0054) - 97165..97890 (-) 726 WP_012485738.1 lysophospholipid acyltransferase family protein -
  CJA_RS00275 (CJA_0055) gmhB 97906..98478 (-) 573 WP_041550781.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  CJA_RS00280 (CJA_0056) glyS 98641..100719 (-) 2079 WP_012485740.1 glycine--tRNA ligase subunit beta -
  CJA_RS00285 (CJA_0057) glyQ 100719..101690 (-) 972 WP_012485741.1 glycine--tRNA ligase subunit alpha -
  CJA_RS00290 (CJA_0058) - 101967..102533 (+) 567 WP_012485742.1 CBS domain-containing protein -
  CJA_RS00295 (CJA_0059) - 102734..103408 (-) 675 WP_012485743.1 hypothetical protein -
  CJA_RS00300 (CJA_0060) - 103550..104446 (+) 897 WP_041550783.1 lysophospholipid acyltransferase family protein -
  CJA_RS00305 (CJA_0061) - 104630..105430 (+) 801 WP_012485745.1 ATP-dependent zinc protease -
  CJA_RS00310 (CJA_0062) - 105463..107013 (+) 1551 WP_012485746.1 inactive transglutaminase family protein -
  CJA_RS00315 (CJA_0063) - 107010..108023 (+) 1014 WP_012485747.1 alpha-L-glutamate ligase-like protein -
  CJA_RS00320 (CJA_0064) - 108133..108696 (+) 564 WP_012485748.1 porin family protein -
  CJA_RS00325 (CJA_0066) - 109061..110407 (+) 1347 WP_083766803.1 glycosyltransferase family 4 protein -
  CJA_RS00330 (CJA_0067) - 110394..111143 (+) 750 WP_012485751.1 class I SAM-dependent methyltransferase -
  CJA_RS00335 (CJA_0068) - 111140..112210 (+) 1071 WP_012485752.1 hypothetical protein -
  CJA_RS00340 (CJA_0070) - 112213..112938 (+) 726 WP_012485753.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CJA_RS00345 (CJA_0069) - 112935..113285 (-) 351 WP_012485754.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  CJA_RS00350 (CJA_0071) - 113367..113981 (-) 615 WP_012485755.1 hypothetical protein -
  CJA_RS00355 (CJA_0072) - 114019..114498 (-) 480 WP_012485756.1 chemotaxis protein CheW -
  CJA_RS00360 (CJA_0073) - 114520..115434 (-) 915 WP_238526799.1 chemotaxis protein CheB -
  CJA_RS00365 (CJA_0074) - 115439..122440 (-) 7002 WP_041550786.1 Hpt domain-containing protein -
  CJA_RS00370 (CJA_0075) - 122459..123316 (-) 858 WP_041550789.1 CheR family methyltransferase -
  CJA_RS00375 (CJA_0076) - 123436..125601 (-) 2166 WP_012485760.1 methyl-accepting chemotaxis protein -
  CJA_RS00380 (CJA_0077) - 125707..126249 (-) 543 WP_041550790.1 chemotaxis protein CheW -
  CJA_RS00385 (CJA_0078) pilH 126372..126734 (-) 363 WP_012485762.1 twitching motility response regulator PilH -
  CJA_RS00390 (CJA_0080) pilG 126864..127259 (-) 396 WP_007639219.1 twitching motility response regulator PilG Regulator
  CJA_RS00395 (CJA_0081) gshB 127605..128558 (+) 954 WP_012485764.1 glutathione synthase -
  CJA_RS00400 (CJA_0082) - 128581..129480 (+) 900 WP_012485765.1 energy transducer TonB -
  CJA_RS00405 (CJA_0083) - 129546..130142 (+) 597 WP_049765371.1 YqgE/AlgH family protein -
  CJA_RS00410 (CJA_0084) ruvX 130146..130577 (+) 432 WP_012485767.1 Holliday junction resolvase RuvX -
  CJA_RS00415 (CJA_0085) - 130589..130915 (-) 327 WP_012485768.1 YegP family protein -
  CJA_RS00420 (CJA_0086) pilU 130948..132093 (-) 1146 WP_012485769.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CJA_RS00425 (CJA_0087) pilT 132115..133149 (-) 1035 WP_012485770.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38166.78 Da        Isoelectric Point: 6.8322

>NTDB_id=31112 CJA_RS00425 WP_012485770.1 132115..133149(-) (pilT) [Cellvibrio japonicus Ueda107]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKQVHGLIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGNVFRDICAVPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYEHVLTIED
PIEFVHESKKCLINQREVHRDTHGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHIVFGTLHTTSAAKTIDRI
VDVFPANEKSMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGASLGMQTMDQCL
ADLVARRVISREAAKTKAKFPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=31112 CJA_RS00425 WP_012485770.1 132115..133149(-) (pilT) [Cellvibrio japonicus Ueda107]
ATGGATATCACCGAACTCCTGGCCTTCAGCGCTAAACAGGGCGCGTCTGACTTGCACCTGTCGGCGGGGCTGCCGCCTAT
GATCCGCGTCGATGGTGATGTGCGCCGTATCAACCTGCCGCCCATGGACCATAAGCAGGTGCACGGCTTGATTTACGACA
TTATGAATGACAAGCAGCGCAAGGACTACGAAGAATTTCTGGAAACCGATTTCTCCTTCGAGGTACCCGGTGTAGCGCGC
TTCCGTGTCAATGCCTTTAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCCAAGGTGCTCACAATGGA
AGAGCTGGGCATGGGCAATGTGTTCCGCGATATCTGCGCGGTTCCGCGCGGTTTGGTATTGGTCACCGGCCCTACCGGTT
CGGGTAAGTCCACCACCCTGGCGGCGATGATCGACTATATCAACGACAACCGCTACGAGCACGTACTCACTATCGAAGAT
CCTATTGAATTCGTGCACGAATCCAAAAAATGCCTGATCAACCAGCGCGAGGTACATCGCGATACCCACGGCTTTAACGA
AGCCCTGCGCTCGGCCCTGCGCGAAGACCCCGATGTGATCCTGGTGGGTGAGATGCGCGACCTGGAAACCATCCGCCTGG
CATTGACGGCAGCCGAAACCGGCCACATCGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAAACCATCGACCGTATT
GTGGACGTGTTCCCGGCCAATGAAAAATCCATGGTGCGCTCCATGCTATCAGAATCACTGCAAGCGGTTATTTCGCAAAC
ACTGCTAAAGAAAAATGGCGGCGGTCGGGTGGCAGCTCACGAAATCATGCGCGGCACCTCAGCAATCCGCAACCTGATCC
GCGAAGATAAGGTAGCGCAGATGTACTCGGCGATCCAGACCGGTGCTTCACTCGGTATGCAGACCATGGATCAGTGCCTT
GCCGATCTGGTCGCGCGCCGGGTAATCAGCCGCGAAGCGGCAAAAACCAAAGCCAAGTTCCCCGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B3PFH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

74.128

100

0.741
  pilT Legionella pneumophila strain ERS1305867

74.128

100

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment