Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D0Y50_RS14845 Genome accession   NZ_CP031769
Coordinates   3369506..3370543 (-) Length   345 a.a.
NCBI ID   WP_108567514.1    Uniprot ID   A0A346NPR3
Organism   Salinimonas sediminis strain N102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3364506..3375543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0Y50_RS19575 - 3364842..3365015 (+) 174 WP_170145423.1 hypothetical protein -
  D0Y50_RS14815 (D0Y50_14815) - 3365133..3365861 (+) 729 WP_117317659.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  D0Y50_RS14820 (D0Y50_14820) gshB 3366033..3367001 (+) 969 WP_117317661.1 glutathione synthase -
  D0Y50_RS14825 (D0Y50_14825) - 3366998..3367564 (+) 567 WP_117317663.1 YqgE/AlgH family protein -
  D0Y50_RS14830 (D0Y50_14830) ruvX 3367557..3367985 (+) 429 WP_108567517.1 Holliday junction resolvase RuvX -
  D0Y50_RS14835 (D0Y50_14835) - 3368024..3368386 (-) 363 WP_108567516.1 DUF2007 domain-containing protein -
  D0Y50_RS14840 (D0Y50_14840) pilU 3368389..3369495 (-) 1107 WP_232366273.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0Y50_RS14845 (D0Y50_14845) pilT 3369506..3370543 (-) 1038 WP_108567514.1 type IV pilus twitching motility protein PilT Machinery gene
  D0Y50_RS14850 (D0Y50_14850) - 3370573..3371262 (+) 690 WP_117317667.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0Y50_RS14855 (D0Y50_14855) proC 3371305..3372123 (+) 819 WP_117317669.1 pyrroline-5-carboxylate reductase -
  D0Y50_RS14860 (D0Y50_14860) - 3372145..3372687 (+) 543 WP_108567511.1 YggT family protein -
  D0Y50_RS14865 (D0Y50_14865) - 3372726..3373160 (+) 435 WP_117317671.1 DUF4426 domain-containing protein -
  D0Y50_RS14870 (D0Y50_14870) - 3373549..3374184 (-) 636 WP_117317673.1 glutathione S-transferase family protein -
  D0Y50_RS14875 (D0Y50_14875) yaaA 3374223..3374999 (-) 777 WP_117317675.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38277.88 Da        Isoelectric Point: 6.5056

>NTDB_id=311074 D0Y50_RS14845 WP_108567514.1 3369506..3370543(-) (pilT) [Salinimonas sediminis strain N102]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGQMRRLNIPELDHKQVHALIYEIMNDNQRKEYEENLEIDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSRVLTLDELGAPEIFKDIIDQPTGLVLVTGATGSGKSTTLAAMVDHINAHKRDHILTIED
PIEFVHDNKLSVINQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAMSAAETGHLVFGTLHTNSAPKTIDRI
VDVFPAAEKSMIRSMLSESLRAVISQSLLKKIGGGRVAAHEIMLGVPAIRNLIREDKVPQMYSVIQTGQSLGMQTMDQCL
QRLVAKGVVSQQDAAAKSVDKQNSF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=311074 D0Y50_RS14845 WP_108567514.1 3369506..3370543(-) (pilT) [Salinimonas sediminis strain N102]
GTGGATATTACCGAACTTTTAGCTTTCAGTGTAAAGAATAACGCGTCAGATTTGCACCTGTCAGCTGGCCTGCCGCCGAT
TATCCGGGTTGACGGACAAATGCGCAGACTGAATATCCCTGAGCTGGATCATAAGCAGGTGCATGCGCTGATTTACGAAA
TCATGAATGATAATCAGCGTAAAGAATACGAAGAAAACCTGGAAATCGATTTTTCATTCGAAGTGAAAGACTTATCCCGT
TTCCGGGTCAATGCCTTTGTGCAAAACCGCGGCGCCGCGGCAGTTCTGAGAACCATACCGTCGCGGGTATTGACCTTGGA
TGAACTGGGAGCACCGGAAATATTTAAAGATATTATCGACCAGCCTACCGGCCTGGTGTTAGTTACCGGCGCTACCGGTT
CGGGGAAAAGTACCACGCTGGCCGCAATGGTTGACCATATTAATGCCCATAAACGCGATCATATCCTGACCATTGAAGAT
CCTATCGAATTTGTCCACGACAACAAGCTTAGTGTCATCAATCAGCGGGAAGTGCACCGTGATACTCACAGTTTTAACAA
TGCGCTTCGTTCCGCATTACGTGAAGATCCGGATGTCATTCTGGTGGGTGAGCTACGTGACCTTGAAACCATTCGGTTAG
CCATGTCAGCCGCAGAAACCGGCCATTTAGTGTTTGGTACCCTGCATACAAACTCCGCGCCGAAAACGATTGACCGTATT
GTCGATGTATTCCCGGCCGCAGAAAAATCAATGATTCGCTCAATGTTGTCTGAGTCATTACGGGCGGTCATCTCACAGTC
ACTGCTGAAAAAAATTGGTGGGGGCAGGGTTGCCGCGCACGAGATAATGCTGGGCGTACCGGCTATTCGTAACCTGATTC
GAGAAGATAAAGTTCCGCAAATGTATTCGGTTATTCAAACCGGTCAATCATTGGGCATGCAAACAATGGATCAGTGCTTA
CAGCGCCTGGTGGCTAAAGGTGTGGTTAGCCAACAGGATGCCGCAGCTAAGTCCGTCGATAAACAAAACAGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346NPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.636

99.42

0.742
  pilT Pseudomonas stutzeri DSM 10701

74.344

99.42

0.739
  pilT Acinetobacter baumannii D1279779

74.926

98.261

0.736
  pilT Acinetobacter baumannii strain A118

74.926

98.261

0.736
  pilT Acinetobacter nosocomialis M2

74.631

98.261

0.733
  pilT Acinetobacter baylyi ADP1

74.702

97.391

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

97.681

0.696
  pilT Vibrio cholerae strain A1552

71.217

97.681

0.696
  pilT Legionella pneumophila strain Lp02

70.088

98.841

0.693
  pilT Legionella pneumophila strain ERS1305867

70.088

98.841

0.693
  pilT Neisseria meningitidis 8013

65.507

100

0.655
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.77

99.42

0.525
  pilU Vibrio cholerae strain A1552

42.687

97.101

0.414
  pilU Acinetobacter baylyi ADP1

40.058

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40

97.101

0.388

Multiple sequence alignment