Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D0C16_RS16180 Genome accession   NZ_CP031728
Coordinates   3827462..3828496 (+) Length   344 a.a.
NCBI ID   WP_151033308.1    Uniprot ID   -
Organism   Cellvibrio sp. KY-GH-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3822462..3833496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0C16_RS16160 (D0C16_16420) - 3822942..3824963 (-) 2022 WP_191968515.1 dynamin family protein -
  D0C16_RS16165 (D0C16_16425) - 3825211..3825804 (-) 594 WP_151033305.1 YggT family protein -
  D0C16_RS16170 (D0C16_16430) proC 3825834..3826655 (-) 822 WP_151033306.1 pyrroline-5-carboxylate reductase -
  D0C16_RS16175 (D0C16_16435) - 3826712..3827398 (-) 687 WP_151033307.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0C16_RS16180 (D0C16_16440) pilT 3827462..3828496 (+) 1035 WP_151033308.1 type IV pilus twitching motility protein PilT Machinery gene
  D0C16_RS16185 (D0C16_16445) pilU 3828567..3829712 (+) 1146 WP_151033309.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0C16_RS16190 (D0C16_16450) ruvX 3829781..3830194 (-) 414 WP_151033310.1 Holliday junction resolvase RuvX -
  D0C16_RS16195 (D0C16_16455) - 3830214..3830807 (-) 594 WP_151033311.1 YqgE/AlgH family protein -
  D0C16_RS16200 (D0C16_16460) - 3830871..3831791 (-) 921 WP_151033312.1 energy transducer TonB -
  D0C16_RS16205 (D0C16_16465) gshB 3831812..3832765 (-) 954 WP_151033313.1 glutathione synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38172.80 Da        Isoelectric Point: 6.8323

>NTDB_id=310796 D0C16_RS16180 WP_151033308.1 3827462..3828496(+) (pilT) [Cellvibrio sp. KY-GH-1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGNVFRDICAVPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHVLTIED
PIEFVHESKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPANEKSMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGASLGMQTMDQCL
ADLVSRRIVSREAAKSKAKFPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=310796 D0C16_RS16180 WP_151033308.1 3827462..3828496(+) (pilT) [Cellvibrio sp. KY-GH-1]
ATGGATATCACCGAACTCCTGGCTTTTAGCGCCAAACAAGGCGCATCGGACTTGCACTTGTCGGCGGGTTTGCCGCCCAT
GATCCGTGTGGATGGCGATGTGCGCCGCATCAACCTGCCGCCCATGGAGCACAAGCAGGTACACGGCTTGATTTACGACA
TTATGAATGACAAGCAGCGCAAGGACTACGAAGAATTCCTGGAAACCGACTTTTCGTTTGAAGTTCCGGGCGTGGCACGC
TTCCGTGTTAACGCCTTTAACCAGAACCGCGGCGCCGGCGCCGTGTTTCGTACCATTCCCTCCAAAGTACTCACTATGGA
AGAGTTGGGGATGGGCAATGTGTTCCGCGATATCTGCGCGGTACCACGCGGTTTGGTGTTGGTAACCGGTCCCACGGGTT
CGGGTAAGTCCACTACCCTCGCCGCTATGATTGATTACATCAACGACAACAAATACGAACACGTATTAACCATCGAAGAC
CCGATCGAATTCGTACACGAATCCAAAAAATGCCTCGTCAACCAACGCGAAGTTCACCGCGATACCCACGGTTTTAACGA
AGCACTCCGCTCCGCCCTCCGCGAAGACCCGGATATTATTCTGGTAGGTGAAATGCGCGATCTGGAAACCATCCGCCTTG
CGCTCACCGCCGCAGAAACCGGTCACTTGGTGTTCGGCACCTTGCACACCACCTCCGCCGCAAAAACCATCGACCGTATT
GTGGACGTGTTCCCTGCGAACGAAAAATCCATGGTGCGCTCAATGCTGTCCGAATCGCTGCAAGCGGTTATCTCGCAGAC
CTTGATGAAAAAAAATGGCGGCGGTCGTGTTGCTGCGCACGAAATCATGCGCGGTACATCAGCAATTCGCAATTTGATCC
GCGAAGATAAAGTGGCGCAAATGTATTCGGCAATTCAAACCGGTGCATCTCTGGGTATGCAGACCATGGATCAGTGTCTG
GCCGATTTGGTATCACGTCGCATTGTCAGTCGCGAAGCGGCCAAGTCGAAGGCCAAGTTCCCCGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

81.105

100

0.811
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

39.823

98.547

0.392
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384

Multiple sequence alignment