Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D0C16_RS16185 Genome accession   NZ_CP031728
Coordinates   3828567..3829712 (+) Length   381 a.a.
NCBI ID   WP_151033309.1    Uniprot ID   A0A5J6PA41
Organism   Cellvibrio sp. KY-GH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3823567..3834712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0C16_RS16165 (D0C16_16425) - 3825211..3825804 (-) 594 WP_151033305.1 YggT family protein -
  D0C16_RS16170 (D0C16_16430) proC 3825834..3826655 (-) 822 WP_151033306.1 pyrroline-5-carboxylate reductase -
  D0C16_RS16175 (D0C16_16435) - 3826712..3827398 (-) 687 WP_151033307.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0C16_RS16180 (D0C16_16440) pilT 3827462..3828496 (+) 1035 WP_151033308.1 type IV pilus twitching motility protein PilT Machinery gene
  D0C16_RS16185 (D0C16_16445) pilU 3828567..3829712 (+) 1146 WP_151033309.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0C16_RS16190 (D0C16_16450) ruvX 3829781..3830194 (-) 414 WP_151033310.1 Holliday junction resolvase RuvX -
  D0C16_RS16195 (D0C16_16455) - 3830214..3830807 (-) 594 WP_151033311.1 YqgE/AlgH family protein -
  D0C16_RS16200 (D0C16_16460) - 3830871..3831791 (-) 921 WP_151033312.1 energy transducer TonB -
  D0C16_RS16205 (D0C16_16465) gshB 3831812..3832765 (-) 954 WP_151033313.1 glutathione synthase -
  D0C16_RS16210 (D0C16_16470) pilG 3833118..3833513 (+) 396 WP_007639219.1 twitching motility response regulator PilG Regulator
  D0C16_RS16215 (D0C16_16475) pilH 3833648..3834010 (+) 363 WP_151033314.1 twitching motility response regulator PilH -
  D0C16_RS16220 (D0C16_16480) - 3834146..3834688 (+) 543 WP_151033315.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42489.83 Da        Isoelectric Point: 6.4423

>NTDB_id=310797 D0C16_RS16185 WP_151033309.1 3828567..3829712(+) (pilU) [Cellvibrio sp. KY-GH-1]
MDFDRLLTLMVEKGASDLFITAGVPPSIKVHGKIVPVTATPLAPEKARELVLSVMNEKQRTEFLENKELNFAVSARGIGR
FRASAFYQRNLAGMVLRRIETKIPTIDELNLPEVIKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNQNSKGHIISIED
PIEFIHQHQGCIITQREVGIDTESFEIALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLKAIVAQQLIPTPDGNGRRACLEIMINTPLAQDLIRKGEVSDLKELMKRSTELGMQTFDQA
LYALYDNGEITYEDALLHADSPNDLRLMIKLGSETDANYLSHAADSLSIQSDEHTNRGKMF

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=310797 D0C16_RS16185 WP_151033309.1 3828567..3829712(+) (pilU) [Cellvibrio sp. KY-GH-1]
ATGGATTTTGATCGGTTATTAACACTAATGGTTGAGAAGGGCGCGTCAGATTTATTTATTACCGCCGGTGTTCCTCCGTC
GATCAAAGTTCATGGCAAAATTGTGCCTGTGACGGCCACGCCGCTCGCCCCGGAAAAAGCGCGCGAGCTGGTGCTCAGTG
TCATGAATGAAAAACAGCGCACCGAATTTCTGGAAAACAAGGAACTTAACTTTGCCGTGAGTGCACGTGGGATTGGCCGC
TTCCGTGCCAGCGCATTTTACCAGCGCAACCTGGCGGGGATGGTATTGCGTCGCATCGAAACCAAGATCCCAACAATCGA
CGAATTAAACCTGCCGGAAGTGATCAAAGAGCTGGCGATGACCAAACGCGGCTTGATTATTTTTGTGGGCGCTACCGGTA
CCGGTAAATCAACCTCGCTAGCCTCCATGATTGGTCATCGCAACCAGAACTCCAAAGGCCATATTATTTCTATCGAAGAC
CCGATTGAGTTTATTCACCAGCATCAGGGTTGCATTATCACTCAGCGCGAAGTAGGAATTGACACTGAATCGTTTGAAAT
TGCGCTGAAAAACACCCTGCGGCAGGCACCAGATGTAATTTTGATTGGCGAGGTACGCTCGCGTGAAACCATGGACCATG
CGATCGCGTTTGCGGAAACTGGTCACTTGTGTTTGTGTACACTCCACGCCAACAACGCCAACCAAGCCTTGGACCGAATC
ATTCACTTCTTCCCGGCTGATCGCCATCGCCAATTGTGGATGGATTTGTCGCTCAACTTAAAAGCGATTGTTGCGCAGCA
GTTGATTCCAACTCCCGATGGAAACGGCCGCCGCGCCTGTCTCGAAATTATGATCAATACGCCACTCGCACAGGATTTGA
TTCGCAAAGGTGAAGTATCAGATTTGAAAGAGTTGATGAAGCGCTCAACCGAATTGGGCATGCAAACATTCGACCAGGCG
CTTTATGCACTTTACGACAATGGCGAAATTACTTATGAAGATGCATTGTTGCACGCTGACTCACCTAACGATTTGCGTCT
GATGATTAAGCTCGGTTCCGAAACCGATGCAAACTATTTGTCGCATGCTGCAGACAGTTTGTCGATCCAGAGTGATGAAC
ACACGAATCGCGGCAAAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6PA41

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

78.571

99.213

0.78
  pilU Acinetobacter baylyi ADP1

69.188

93.701

0.648
  pilU Vibrio cholerae strain A1552

55.714

91.864

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.768

90.551

0.396
  pilT Pseudomonas aeruginosa PAK

41.159

90.551

0.373
  pilT Acinetobacter nosocomialis M2

40.351

89.764

0.362
  pilT Acinetobacter baumannii D1279779

40.351

89.764

0.362
  pilT Acinetobacter baumannii strain A118

40.351

89.764

0.362

Multiple sequence alignment