Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D0B88_RS03430 Genome accession   NZ_CP031727
Coordinates   792428..793462 (+) Length   344 a.a.
NCBI ID   WP_007639272.1    Uniprot ID   I3IEU9
Organism   Cellvibrio sp. KY-YJ-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 788589..804090 792428..793462 within 0


Gene organization within MGE regions


Location: 788589..804090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0B88_RS03405 (D0B88_03425) - 788589..789299 (-) 711 WP_151055050.1 anti-sigma factor domain-containing protein -
  D0B88_RS03410 (D0B88_03430) - 789296..789838 (-) 543 WP_151055053.1 sigma-70 family RNA polymerase sigma factor -
  D0B88_RS03415 (D0B88_03435) - 790086..790685 (-) 600 WP_151055054.1 YggT family protein -
  D0B88_RS03420 (D0B88_03440) proC 790773..791621 (-) 849 WP_151055057.1 pyrroline-5-carboxylate reductase -
  D0B88_RS03425 (D0B88_03445) - 791651..792352 (-) 702 WP_151055059.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0B88_RS03430 (D0B88_03450) pilT 792428..793462 (+) 1035 WP_007639272.1 type IV pilus twitching motility protein PilT Machinery gene
  D0B88_RS03435 (D0B88_03455) pilU 793553..794698 (+) 1146 WP_007639271.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B88_RS03440 (D0B88_03460) - 794822..795547 (-) 726 WP_151055062.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  D0B88_RS03445 (D0B88_03465) - 795604..796692 (-) 1089 WP_151055063.1 prenyltransferase -
  D0B88_RS03450 (D0B88_03470) - 796689..797402 (-) 714 WP_151055066.1 class I SAM-dependent methyltransferase -
  D0B88_RS03455 (D0B88_03475) - 797389..798774 (-) 1386 WP_151055068.1 glycosyltransferase family 4 protein -
  D0B88_RS03460 (D0B88_03480) - 798865..799950 (-) 1086 WP_225318630.1 DUF5924 family protein -
  D0B88_RS03465 (D0B88_03490) - 800615..801649 (-) 1035 WP_007639262.1 alpha-L-glutamate ligase-like protein -
  D0B88_RS03470 (D0B88_03495) - 801646..803229 (-) 1584 WP_007639261.1 inactive transglutaminase family protein -
  D0B88_RS03475 (D0B88_03500) - 803296..804090 (-) 795 WP_225318518.1 RimK/LysX family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38170.83 Da        Isoelectric Point: 6.8321

>NTDB_id=310757 D0B88_RS03430 WP_007639272.1 792428..793462(+) (pilT) [Cellvibrio sp. KY-YJ-3]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGNVFRDICAVPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHVLTIED
PIEFVHESKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPANEKSMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGASLGMQTMDQCL
ADLVARRIISRDAAKSKAKFPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=310757 D0B88_RS03430 WP_007639272.1 792428..793462(+) (pilT) [Cellvibrio sp. KY-YJ-3]
ATGGATATCACCGAACTGCTGGCGTTTAGTGCCAAACAAGGCGCGTCTGACTTGCACCTCTCGGCGGGTTTGCCGCCGAT
GATTCGTGTCGACGGCGACGTGCGCCGTATCAACCTGCCGCCGATGGAGCACAAGCAGGTGCACGGCTTGATTTACGACA
TTATGAATGACAAGCAGCGCAAGGACTACGAAGAATTTCTGGAAACCGACTTCTCGTTCGAAGTGCCCGGTGTGGCCCGC
TTCCGGGTAAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCCAAAGTATTAACGATGGA
AGAATTGGGCATGGGCAATGTGTTCCGCGATATCTGCGCGGTACCGCGTGGATTAGTGTTAGTGACCGGGCCGACCGGTT
CGGGTAAATCCACCACGCTCGCGGCGATGATCGACTACATCAACGACAACAAATACGAACACGTACTCACCATTGAAGAC
CCGATTGAATTTGTCCACGAATCCAAAAAGTGTTTGATCAACCAACGTGAAGTGCACCGCGATACCCACGGTTTTAACGA
AGCACTGCGTTCGGCACTGCGTGAAGACCCGGATATTATTCTCGTCGGTGAGATGCGCGATTTGGAAACCATTCGTTTGG
CACTCACCGCCGCCGAAACCGGCCACTTGGTATTCGGTACCTTGCACACGACCTCCGCTGCAAAAACCATCGACCGTATT
GTGGACGTATTCCCCGCGAACGAAAAATCCATGGTGCGTTCAATGTTGTCGGAATCGCTGCAAGCGGTAATCTCACAAAC
CCTGATGAAGAAAAATGGCGGCGGCCGTGTCGCGGCGCACGAAATTATGCGCGGCACCTCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTCGCGCAAATGTATTCGGCAATCCAAACCGGTGCATCGCTCGGTATGCAGACCATGGATCAGTGCTTG
GCCGATTTGGTCGCGCGCCGTATTATCAGCCGCGATGCCGCCAAATCCAAAGCTAAATTCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3IEU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.953

100

0.89
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Vibrio cholerae strain A1552

39.823

98.547

0.392
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment