Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D0B88_RS03435 Genome accession   NZ_CP031727
Coordinates   793553..794698 (+) Length   381 a.a.
NCBI ID   WP_007639271.1    Uniprot ID   I3IEU8
Organism   Cellvibrio sp. KY-YJ-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 788589..804090 793553..794698 within 0


Gene organization within MGE regions


Location: 788589..804090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0B88_RS03405 (D0B88_03425) - 788589..789299 (-) 711 WP_151055050.1 anti-sigma factor domain-containing protein -
  D0B88_RS03410 (D0B88_03430) - 789296..789838 (-) 543 WP_151055053.1 sigma-70 family RNA polymerase sigma factor -
  D0B88_RS03415 (D0B88_03435) - 790086..790685 (-) 600 WP_151055054.1 YggT family protein -
  D0B88_RS03420 (D0B88_03440) proC 790773..791621 (-) 849 WP_151055057.1 pyrroline-5-carboxylate reductase -
  D0B88_RS03425 (D0B88_03445) - 791651..792352 (-) 702 WP_151055059.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0B88_RS03430 (D0B88_03450) pilT 792428..793462 (+) 1035 WP_007639272.1 type IV pilus twitching motility protein PilT Machinery gene
  D0B88_RS03435 (D0B88_03455) pilU 793553..794698 (+) 1146 WP_007639271.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D0B88_RS03440 (D0B88_03460) - 794822..795547 (-) 726 WP_151055062.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  D0B88_RS03445 (D0B88_03465) - 795604..796692 (-) 1089 WP_151055063.1 prenyltransferase -
  D0B88_RS03450 (D0B88_03470) - 796689..797402 (-) 714 WP_151055066.1 class I SAM-dependent methyltransferase -
  D0B88_RS03455 (D0B88_03475) - 797389..798774 (-) 1386 WP_151055068.1 glycosyltransferase family 4 protein -
  D0B88_RS03460 (D0B88_03480) - 798865..799950 (-) 1086 WP_225318630.1 DUF5924 family protein -
  D0B88_RS03465 (D0B88_03490) - 800615..801649 (-) 1035 WP_007639262.1 alpha-L-glutamate ligase-like protein -
  D0B88_RS03470 (D0B88_03495) - 801646..803229 (-) 1584 WP_007639261.1 inactive transglutaminase family protein -
  D0B88_RS03475 (D0B88_03500) - 803296..804090 (-) 795 WP_225318518.1 RimK/LysX family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42435.78 Da        Isoelectric Point: 6.5040

>NTDB_id=310758 D0B88_RS03435 WP_007639271.1 793553..794698(+) (pilU) [Cellvibrio sp. KY-YJ-3]
MDFDRLLSLMVEKGASDLFITAGVPPSIKVHGKVVPVTATPLAPEKARELVLSVMNEKQRNEFLEKKELNFAVSARGIGR
FRASAFYQRNLAGMVLRRIETKIPQVDELGLPEIIKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNQNSKGHIISIED
PIEFIHQHQGCIITQREVGIDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLKAIVAQQLIPTPDGNGRRACLEIMINTPLAQDLIRKGEVSELKELMKRSTELGMQTFDQA
LYALYDAGEITYEDALLHADSPNDLRLMIKLASETDSNYLSHAADSLSIQSDDQNNRGKMF

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=310758 D0B88_RS03435 WP_007639271.1 793553..794698(+) (pilU) [Cellvibrio sp. KY-YJ-3]
ATGGATTTTGATCGGTTATTGTCCTTGATGGTAGAAAAAGGCGCGTCGGATTTATTTATCACCGCCGGTGTACCGCCTTC
GATCAAAGTCCACGGCAAGGTTGTGCCTGTGACCGCCACTCCACTCGCGCCAGAAAAAGCGCGCGAGCTGGTGCTGAGTG
TGATGAATGAAAAACAGCGTAATGAATTTCTGGAGAAAAAAGAACTTAACTTTGCGGTAAGTGCACGCGGTATTGGCCGT
TTCCGCGCCAGCGCGTTTTACCAGCGCAACCTCGCGGGCATGGTATTGCGCCGTATTGAAACCAAAATTCCGCAAGTGGA
TGAATTGGGCCTACCGGAAATTATTAAAGAATTGGCCATGACCAAACGTGGCCTGATTATTTTTGTAGGCGCGACCGGTA
CCGGTAAATCCACGTCACTCGCCTCCATGATTGGCCATCGCAACCAGAACAGCAAAGGCCATATTATTTCTATCGAAGAC
CCAATTGAATTTATCCACCAACATCAAGGCTGCATCATCACCCAGCGCGAAGTGGGCATTGATACCGAATCCTTTGAGGT
TGCACTGAAAAATACCCTACGCCAAGCGCCCGATGTGATTTTGATTGGTGAGGTGCGCTCGCGCGAAACCATGGATCACG
CCATCGCCTTTGCGGAAACCGGTCACTTGTGTCTGTGTACGCTGCACGCCAACAACGCCAACCAGGCCCTCGACCGTATC
ATTCACTTCTTCCCGGCGGATCGCCATCGCCAATTGTGGATGGATTTATCACTGAACTTAAAAGCGATTGTGGCGCAGCA
ATTAATCCCCACACCCGACGGCAATGGCCGCCGCGCCTGTCTGGAAATTATGATCAACACCCCACTGGCGCAGGATTTAA
TTCGCAAAGGGGAAGTATCAGAATTAAAAGAATTGATGAAACGCTCCACCGAATTAGGAATGCAGACCTTCGACCAAGCG
CTTTATGCACTCTACGACGCCGGTGAAATCACCTACGAAGACGCGCTGCTGCACGCCGACTCACCCAACGACTTGCGCCT
GATGATTAAACTCGCCTCGGAAACCGATTCAAATTATTTGTCCCATGCGGCAGATAGTTTGTCGATCCAATCTGACGATC
AAAATAATCGCGGGAAAATGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3IEU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.778

99.213

0.772
  pilU Acinetobacter baylyi ADP1

68.908

93.701

0.646
  pilU Vibrio cholerae strain A1552

55.714

91.864

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

92.651

0.402
  pilT Pseudomonas aeruginosa PAK

41.159

90.551

0.373
  pilT Acinetobacter nosocomialis M2

41.228

89.764

0.37
  pilT Acinetobacter baumannii D1279779

41.228

89.764

0.37
  pilT Acinetobacter baumannii strain A118

41.228

89.764

0.37
  pilT Legionella pneumophila strain Lp02

41.493

87.927

0.365
  pilT Legionella pneumophila strain ERS1305867

41.493

87.927

0.365
  pilT Acinetobacter baylyi ADP1

40.351

89.764

0.362

Multiple sequence alignment