Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BEN71_RS14515 Genome accession   NZ_CP031716
Coordinates   2911553..2912590 (+) Length   345 a.a.
NCBI ID   WP_068975798.1    Uniprot ID   -
Organism   Acinetobacter wuhouensis strain WCHA60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2906553..2917590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN71_RS14490 (BEN71_14490) - 2907059..2907457 (-) 399 WP_068975793.1 hypothetical protein -
  BEN71_RS14495 (BEN71_14495) - 2907520..2908275 (-) 756 WP_068975794.1 hypothetical protein -
  BEN71_RS14500 (BEN71_14500) - 2908501..2909322 (-) 822 WP_068975795.1 hypothetical protein -
  BEN71_RS14505 (BEN71_14505) - 2909513..2910313 (-) 801 WP_068975796.1 thioesterase family protein -
  BEN71_RS14510 (BEN71_14510) - 2910737..2911429 (-) 693 WP_068975797.1 YggS family pyridoxal phosphate-dependent enzyme -
  BEN71_RS14515 (BEN71_14515) pilT 2911553..2912590 (+) 1038 WP_068975798.1 type IV pilus twitching motility protein PilT Machinery gene
  BEN71_RS14520 (BEN71_14520) pilU 2912614..2913732 (+) 1119 WP_068975799.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BEN71_RS14525 (BEN71_14525) fur 2913817..2914254 (-) 438 WP_068975800.1 ferric iron uptake transcriptional regulator -
  BEN71_RS14530 (BEN71_14530) - 2914367..2914765 (+) 399 WP_068975801.1 outer membrane protein assembly factor BamE -
  BEN71_RS14535 (BEN71_14535) - 2914849..2915169 (-) 321 WP_068975802.1 RnfH family protein -
  BEN71_RS14540 (BEN71_14540) - 2915187..2916269 (-) 1083 WP_213073242.1 hypothetical protein -
  BEN71_RS14545 (BEN71_14545) - 2916502..2916957 (-) 456 WP_068975804.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38247.94 Da        Isoelectric Point: 6.5039

>NTDB_id=310687 BEN71_RS14515 WP_068975798.1 2911553..2912590(+) (pilT) [Acinetobacter wuhouensis strain WCHA60]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEQLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDLCELPRGLILVTGPTGSGKSTTLAAMVDFINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQCLLKKTGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQVARNSAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=310687 BEN71_RS14515 WP_068975798.1 2911553..2912590(+) (pilT) [Acinetobacter wuhouensis strain WCHA60]
ATGGATATTACAGAATTATTGGCATTTTCTGTCAAAAACGGGGCATCAGACTTACACCTTTCAGCTGGTATGCCACCGAT
GATTCGTGTGGATGGTGAAGTTCGCCGTATCAATTTACCAGCTTTAGAACATAAAGATGTTCACCGTCTTGTTTATGACA
TCATGAATGATAAACAACGTCGTGATTTTGAAGAACAATTAGAAACTGACTTTTCGTTTGAAGTCCCTGGTGTTGCCCGT
TTCCGTGTCAACGCATTTAACCAAAACCGTGGTGCAGGCGCTGTATTCCGTACCATTCCTTCTAAAGTTTTAACGATGGA
AGATTTGGGTCTAGGACAAATCTTTAAAGATCTTTGTGAATTACCTCGTGGTTTAATTCTCGTCACAGGGCCTACAGGTT
CGGGGAAGTCAACAACCTTAGCAGCAATGGTTGATTTCATTAATGACAATCGTTATGACCACATCCTCACAGTCGAAGAC
CCGATTGAATTTGTGCACCAGTCGAAGAAATGTTTGATCAACCAGCGTGAAGTGCATCGTGACACCCATGGCTTTAATGA
AGCACTGCGTTCGGCACTGCGTGAAGACCCAGACATCATCCTCGTCGGGGAAATGCGTGACCTTGAAACGATTCGTTTGG
CACTCACAGCTGCGGAAACAGGTCACTTGGTATTTGGTACGCTACATACCACGTCTGCGGCAAAAACCATTGACCGTGTG
ATCGACGTATTCCCAGCTGAAGAAAAAGACATGGTTCGTGCCATGTTATCTGAATCCTTACAAGCTGTTATTTCTCAATG
TCTACTGAAAAAAACAGGTGGTGGACGAGTTGCCGCGCATGAAATCATGATCGGTATTCCTGCAATCCGTAACTTAATCC
GTGAAAACAAAGTTGCTCAAATGTACTCTGCGATTCAAACAGGTGCAAACTATGGTATGACGACATTGGATCAAAGTTTA
AAAACACTGGTATCTAAAGGTGTAATTAGCCCTCAAGTTGCACGAAATTCTGCAAAACAACCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.203

100

0.942
  pilT Acinetobacter baumannii strain A118

94.203

100

0.942
  pilT Acinetobacter baylyi ADP1

85.756

99.71

0.855
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

82.849

99.71

0.826
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.181

96.232

0.675
  pilT Vibrio cholerae strain A1552

70.181

96.232

0.675
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.888

98.261

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.39

95.942

0.397
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394

Multiple sequence alignment