Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BEN71_RS14520 Genome accession   NZ_CP031716
Coordinates   2912614..2913732 (+) Length   372 a.a.
NCBI ID   WP_068975799.1    Uniprot ID   A0A385C6N2
Organism   Acinetobacter wuhouensis strain WCHA60     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2907614..2918732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN71_RS14500 (BEN71_14500) - 2908501..2909322 (-) 822 WP_068975795.1 hypothetical protein -
  BEN71_RS14505 (BEN71_14505) - 2909513..2910313 (-) 801 WP_068975796.1 thioesterase family protein -
  BEN71_RS14510 (BEN71_14510) - 2910737..2911429 (-) 693 WP_068975797.1 YggS family pyridoxal phosphate-dependent enzyme -
  BEN71_RS14515 (BEN71_14515) pilT 2911553..2912590 (+) 1038 WP_068975798.1 type IV pilus twitching motility protein PilT Machinery gene
  BEN71_RS14520 (BEN71_14520) pilU 2912614..2913732 (+) 1119 WP_068975799.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BEN71_RS14525 (BEN71_14525) fur 2913817..2914254 (-) 438 WP_068975800.1 ferric iron uptake transcriptional regulator -
  BEN71_RS14530 (BEN71_14530) - 2914367..2914765 (+) 399 WP_068975801.1 outer membrane protein assembly factor BamE -
  BEN71_RS14535 (BEN71_14535) - 2914849..2915169 (-) 321 WP_068975802.1 RnfH family protein -
  BEN71_RS14540 (BEN71_14540) - 2915187..2916269 (-) 1083 WP_213073242.1 hypothetical protein -
  BEN71_RS14545 (BEN71_14545) - 2916502..2916957 (-) 456 WP_068975804.1 bacteriohemerythrin -
  BEN71_RS14550 (BEN71_14550) - 2917424..2918272 (-) 849 WP_068975805.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41890.31 Da        Isoelectric Point: 6.5074

>NTDB_id=310688 BEN71_RS14520 WP_068975799.1 2912614..2913732(+) (pilU) [Acinetobacter wuhouensis strain WCHA60]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGDIIMQLLNSIMSEKQRKEFAETRECNFAITNKDKTA
RFRVSAFQQRDMPGMVLRRIETKIPTMEELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNHNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGDIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANTKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=310688 BEN71_RS14520 WP_068975799.1 2912614..2913732(+) (pilU) [Acinetobacter wuhouensis strain WCHA60]
ATGGACTTTAATGACTTGCTCAATATGATGATTGAGCAAAAAGCATCCGATTTATTTATTACGGCTGATGTTGAACCATC
AATGAAAATTAATGGTCAAATCGTACCTGTGGTAAAAACCAAGTTGAATGGCGACATCATCATGCAATTGCTCAACTCAA
TTATGAGTGAAAAGCAACGTAAAGAATTTGCCGAAACTCGTGAGTGTAACTTCGCGATTACCAATAAAGACAAGACAGCG
CGTTTTCGTGTCAGCGCATTTCAGCAACGTGATATGCCAGGCATGGTTTTACGTCGTATTGAAACTAAAATTCCAACGAT
GGAAGAGTTAAAACTTCCACCAATTCTAAAAGATTTAGCCATGACCAAACGTGGCATTATTATCTTTGTAGGTGCGACAG
GTACGGGGAAATCAACCTCTTTAGCATCGATTATTGGTTACCGTAACCATAATTCTAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTTCATGAACATGCGGGTTGTATTATTACCCAACGCGAAGTGGGTATTGATACCGATTCATTTGA
AATCGCGTTAAAAAATACCTTACGTCAAGCACCCGATGTAATCTTAATTGGTGAGATTCGCTCTCGTGAAACCATGGATT
ACGCGATTGCCTTTGCGGAAACGGGTCACTTGGTTTTTGCTACACTGCACGCCAACAACGCCAACCAAGCCATTGATCGT
ATTATTCACTTCTTTGAATCTGACCGTCATAGCCAATTGTTCATGGACTTGTCGCTGAATTTAAAAGCGATGATCGCGCA
ACAGTTGATTCCAACACCAGATGGTAACTCTCGTCGTGCTGCGATTGAAATTCTAATCAACTCCCCTTTAATTTCTGATT
TGATCCGTAAAGGTGACATTCACGAAATTAAAGATTTGATGAAACGCTCACGTGAGTTAGGGATGCAAACCTTTGACCAA
GCCTTGTTTGACTTATATAAAGCAGGGCAAATCACCTATAAAGATGCACTGAAACATGCAGATTCACCAAATGATTTACG
TCTACAAATCAAACTGGATGAAGAAGGTGGTGCACAATTGCTCAATGCAAATACTAAATTAACTTTCGACGGTCAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385C6N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.78
  pilU Pseudomonas stutzeri DSM 10701

67.655

99.731

0.675
  pilU Vibrio cholerae strain A1552

55.068

98.118

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

93.011

0.401
  pilT Acinetobacter baumannii D1279779

40.525

92.204

0.374
  pilT Acinetobacter baumannii strain A118

40.525

92.204

0.374
  pilT Acinetobacter nosocomialis M2

40.525

92.204

0.374
  pilT Pseudomonas aeruginosa PAK

40.173

93.011

0.374
  pilT Legionella pneumophila strain ERS1305867

41.071

90.323

0.371
  pilT Legionella pneumophila strain Lp02

41.071

90.323

0.371
  pilT Acinetobacter baylyi ADP1

39.942

92.204

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.306

93.011

0.366
  pilT Neisseria meningitidis 8013

39.017

93.011

0.363
  pilT Neisseria gonorrhoeae MS11

39.017

93.011

0.363

Multiple sequence alignment