Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D0T90_RS04515 Genome accession   NZ_CP031699
Coordinates   980838..981881 (+) Length   347 a.a.
NCBI ID   WP_123795730.1    Uniprot ID   A0A5P3MS09
Organism   Neisseria animalis strain ATCC 49930     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 975838..986881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0T90_RS04495 (D0T90_04660) - 977533..977922 (-) 390 WP_123795734.1 hypothetical protein -
  D0T90_RS04500 (D0T90_04665) recX 978049..978516 (+) 468 WP_123795733.1 recombination regulator RecX Regulator
  D0T90_RS04505 (D0T90_04670) rsgA 979082..979948 (-) 867 WP_123795732.1 ribosome small subunit-dependent GTPase A -
  D0T90_RS04510 (D0T90_04675) - 980041..980751 (-) 711 WP_123795731.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0T90_RS04515 (D0T90_04680) pilT 980838..981881 (+) 1044 WP_123795730.1 type IV pilus twitching motility protein PilT Machinery gene
  D0T90_RS04520 (D0T90_04685) - 981978..983207 (+) 1230 WP_123795729.1 PilT/PilU family type 4a pilus ATPase -
  D0T90_RS04525 (D0T90_04690) - 983264..983689 (+) 426 WP_123795728.1 CopD family protein -
  D0T90_RS04530 (D0T90_04695) - 983710..984186 (+) 477 WP_123795727.1 CYTH domain-containing protein -
  D0T90_RS04535 (D0T90_04705) - 984407..985612 (-) 1206 WP_123795726.1 chloride channel protein -
  D0T90_RS04540 (D0T90_04710) - 986134..986622 (+) 489 WP_123795725.1 dihydrofolate reductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38298.72 Da        Isoelectric Point: 7.0253

>NTDB_id=310509 D0T90_RS04515 WP_123795730.1 980838..981881(+) (pilT) [Neisseria animalis strain ATCC 49930]
MQITDLLAFGVKNQASDLHLSAGMPPMIRVNGDIRRINLPEMDADEVGSLIDSVMTDHQRKAYRQDLETDFSFELPNVAR
FRANVFTTDRGPAAVFRTIPNTVLTLEDLGAPRILQKIADSPRGLVLVTGPTGSGKSTTLAAMIDYINKNQPAHILTIED
PVEFIHHSQKALINQRELHRHTHSFHNALRSALREDPDVILVGEMRDPETIRLALTAAETGHLVFATLHTTGAAKTIDRI
VDVFPAGEKEMVRAMLSESLRAVISQILLKKRDGNGRIAAHEILMSTPAVRNLIRENKTAQLQSALQTGHSHGMQTLDQA
LQQLLRQGLISAETARSKAQNSDYLAD

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=310509 D0T90_RS04515 WP_123795730.1 980838..981881(+) (pilT) [Neisseria animalis strain ATCC 49930]
ATGCAGATTACCGACTTACTCGCTTTCGGTGTAAAAAACCAAGCCTCCGATCTCCATTTGAGTGCCGGTATGCCGCCGAT
GATTCGCGTCAACGGAGACATCCGCCGCATCAACCTGCCTGAAATGGACGCAGACGAAGTCGGCAGCCTGATTGATTCGG
TCATGACCGACCACCAGCGCAAGGCATACCGGCAGGATTTGGAAACGGATTTTTCGTTTGAGCTGCCGAATGTGGCGCGC
TTCCGTGCCAATGTATTCACCACCGACCGCGGGCCTGCCGCCGTTTTCCGTACCATTCCCAACACGGTGTTGACGCTGGA
AGATTTGGGTGCCCCGCGCATTTTGCAAAAAATCGCCGACTCGCCGCGCGGCTTGGTATTGGTTACCGGCCCTACCGGCT
CGGGAAAATCCACCACGCTGGCAGCGATGATTGACTACATCAACAAAAACCAACCGGCCCATATCCTGACTATTGAAGAT
CCGGTTGAGTTTATACACCACAGCCAAAAAGCGCTCATCAACCAACGCGAACTGCACCGCCATACCCACAGCTTCCACAA
CGCCCTGCGCTCGGCTCTGCGCGAAGATCCCGATGTGATTCTGGTGGGCGAAATGCGCGATCCGGAAACCATACGGCTGG
CACTGACCGCTGCCGAAACCGGCCATCTGGTTTTCGCCACCCTGCATACCACCGGCGCGGCAAAAACCATAGACCGCATT
GTCGATGTTTTTCCCGCCGGAGAAAAAGAAATGGTTCGCGCAATGCTGTCCGAATCACTGCGTGCCGTGATTTCACAAAT
TCTGCTGAAAAAACGCGACGGCAACGGACGGATTGCCGCACACGAAATCCTGATGTCCACTCCGGCAGTACGCAATCTGA
TTCGCGAAAACAAAACCGCCCAGCTCCAATCCGCCCTGCAAACCGGCCACAGCCACGGTATGCAGACTTTGGACCAAGCA
CTGCAACAACTGCTGCGGCAAGGTCTGATTAGTGCCGAAACCGCCAGAAGCAAAGCACAAAACAGCGATTATCTTGCCGA
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P3MS09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

78.035

99.712

0.778
  pilT Neisseria gonorrhoeae MS11

78.035

99.712

0.778
  pilT Acinetobacter nosocomialis M2

70.588

97.983

0.692
  pilT Acinetobacter baumannii D1279779

70.588

97.983

0.692
  pilT Acinetobacter baumannii strain A118

70.588

97.983

0.692
  pilT Acinetobacter baylyi ADP1

69.679

98.847

0.689
  pilT Pseudomonas stutzeri DSM 10701

69.706

97.983

0.683
  pilT Pseudomonas aeruginosa PAK

68.529

97.983

0.671
  pilT Legionella pneumophila strain Lp02

64.809

98.271

0.637
  pilT Legionella pneumophila strain ERS1305867

64.809

98.271

0.637
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.118

97.983

0.628
  pilT Vibrio cholerae strain A1552

64.118

97.983

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.737

97.695

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.029

99.424

0.418
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Vibrio cholerae strain A1552

38.551

99.424

0.383

Multiple sequence alignment