Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DXT74_RS03940 Genome accession   NZ_CP031514
Coordinates   847858..848895 (+) Length   345 a.a.
NCBI ID   WP_043639309.1    Uniprot ID   A0A1W0CSM2
Organism   Chromobacterium sp. Rain0013     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842858..853895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXT74_RS03910 (DXT74_03885) aroG 843068..844129 (-) 1062 WP_019102527.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  DXT74_RS03915 (DXT74_03890) dksA 844361..844771 (-) 411 WP_019102526.1 RNA polymerase-binding protein DksA -
  DXT74_RS03920 (DXT74_03895) - 845056..845361 (-) 306 WP_019102525.1 c-type cytochrome -
  DXT74_RS03925 (DXT74_03900) - 845453..846034 (-) 582 WP_043589318.1 YggT family protein -
  DXT74_RS03930 (DXT74_03905) proC 846043..846846 (-) 804 WP_043639303.1 pyrroline-5-carboxylate reductase -
  DXT74_RS03935 (DXT74_03910) - 846900..847592 (-) 693 WP_194427372.1 YggS family pyridoxal phosphate-dependent enzyme -
  DXT74_RS03940 (DXT74_03915) pilT 847858..848895 (+) 1038 WP_043639309.1 type IV pilus twitching motility protein PilT Machinery gene
  DXT74_RS03945 (DXT74_03920) pilU 848941..850077 (+) 1137 WP_043639312.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DXT74_RS03950 (DXT74_03925) - 850208..850795 (-) 588 WP_043639315.1 TetR/AcrR family transcriptional regulator -
  DXT74_RS03955 (DXT74_03930) - 851254..853173 (+) 1920 WP_052370646.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38350.14 Da        Isoelectric Point: 6.7774

>NTDB_id=307241 DXT74_RS03940 WP_043639309.1 847858..848895(+) (pilT) [Chromobacterium sp. Rain0013]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPMDHHDVHDMVYDIMNDYQRKIFEDTFECDFSFDLPGIAR
FRVNSFIQNRGMGAVFRVIPSKVLSLEQLGAPKIFQEIAAFPRGLVLVTGPTGSGKSTTLAAMIDFINDNHFSHILTVED
PIEFVHDSKKCLINQRELGLQTHSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKTKDGSGRVAAHEIMLGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LQELVRRNMVSPADARQKAANKDQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=307241 DXT74_RS03940 WP_043639309.1 847858..848895(+) (pilT) [Chromobacterium sp. Rain0013]
ATGGAAATTTCGGAGCTCTTGGCCTTTACGGTCAAGAACAAGGCGTCCGACCTGCACTTGTCCGCCGGGCTGCCGCCGAT
GATCCGGGTGAACGGCGACATCCGTCGCATCAACCTGCCGCCGATGGACCACCATGACGTGCACGACATGGTGTACGACA
TCATGAACGATTACCAGCGCAAAATCTTCGAAGACACCTTCGAGTGCGACTTTTCCTTCGATCTGCCCGGCATCGCCCGT
TTCCGCGTCAACTCATTCATACAGAACCGCGGCATGGGCGCGGTGTTTCGGGTGATTCCGTCCAAGGTGTTATCGCTGGA
ACAGCTGGGCGCGCCCAAGATCTTCCAGGAAATCGCCGCCTTTCCGCGCGGGCTGGTGCTGGTGACCGGCCCCACCGGCT
CCGGCAAATCCACTACGCTGGCGGCGATGATAGATTTCATCAACGACAACCACTTCTCCCACATCCTGACTGTCGAGGAC
CCGATCGAGTTTGTCCACGACAGCAAGAAGTGCCTGATCAACCAGCGTGAACTGGGTTTGCAGACACACAGCTTCGCCAA
TGCCTTGAAGTCCGCCTTGCGGGAAGACCCGGATGTGATCCTGGTGGGCGAACTGCGCGATCTGGAAACCATACGCCTGG
CTTTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCGGCCAAGACCATAGACCGCATC
GTCGACGTGTTCCCGGCCGGGGAAAAGGAGATGGTGCGCTCCATGCTGTCCGAGTCGGTGCGCGCGGTGATCGCCCAGAC
CCTGCTCAAAACCAAGGACGGCAGCGGGCGGGTGGCGGCGCATGAAATCATGCTGGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGATTGCTCAAATCAACTCCATGATCCAGACCGGCCAGCAGCATGGGATGCAGACGCTGGACCAGTGC
CTGCAGGAGCTGGTGCGGCGCAATATGGTGTCGCCGGCGGACGCCAGGCAAAAGGCCGCCAACAAGGATCAATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W0CSM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.174

100

0.722
  pilT Acinetobacter baumannii strain A118

72.174

100

0.722
  pilT Acinetobacter baumannii D1279779

72.174

100

0.722
  pilT Neisseria meningitidis 8013

71.429

99.42

0.71
  pilT Neisseria gonorrhoeae MS11

71.429

99.42

0.71
  pilT Pseudomonas stutzeri DSM 10701

71.014

100

0.71
  pilT Legionella pneumophila strain Lp02

70.725

100

0.707
  pilT Pseudomonas aeruginosa PAK

70.725

100

0.707
  pilT Legionella pneumophila strain ERS1305867

70.725

100

0.707
  pilT Acinetobacter baylyi ADP1

70.435

100

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.568

97.971

0.652
  pilT Vibrio cholerae strain A1552

66.568

97.971

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.888

98.261

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406

Multiple sequence alignment