Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DXT74_RS03945 Genome accession   NZ_CP031514
Coordinates   848941..850077 (+) Length   378 a.a.
NCBI ID   WP_043639312.1    Uniprot ID   A0A1W0CSX0
Organism   Chromobacterium sp. Rain0013     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 843941..855077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXT74_RS03915 (DXT74_03890) dksA 844361..844771 (-) 411 WP_019102526.1 RNA polymerase-binding protein DksA -
  DXT74_RS03920 (DXT74_03895) - 845056..845361 (-) 306 WP_019102525.1 c-type cytochrome -
  DXT74_RS03925 (DXT74_03900) - 845453..846034 (-) 582 WP_043589318.1 YggT family protein -
  DXT74_RS03930 (DXT74_03905) proC 846043..846846 (-) 804 WP_043639303.1 pyrroline-5-carboxylate reductase -
  DXT74_RS03935 (DXT74_03910) - 846900..847592 (-) 693 WP_194427372.1 YggS family pyridoxal phosphate-dependent enzyme -
  DXT74_RS03940 (DXT74_03915) pilT 847858..848895 (+) 1038 WP_043639309.1 type IV pilus twitching motility protein PilT Machinery gene
  DXT74_RS03945 (DXT74_03920) pilU 848941..850077 (+) 1137 WP_043639312.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DXT74_RS03950 (DXT74_03925) - 850208..850795 (-) 588 WP_043639315.1 TetR/AcrR family transcriptional regulator -
  DXT74_RS03955 (DXT74_03930) - 851254..853173 (+) 1920 WP_052370646.1 diguanylate cyclase -
  DXT74_RS03960 (DXT74_03935) - 853215..853922 (+) 708 WP_114153138.1 pseudouridine synthase -
  DXT74_RS03965 (DXT74_03940) - 854093..854470 (+) 378 WP_043639320.1 VOC family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42295.39 Da        Isoelectric Point: 6.2382

>NTDB_id=307242 DXT74_RS03945 WP_043639312.1 848941..850077(+) (pilU) [Chromobacterium sp. Rain0013]
MEKDQASKFILDLLKHATGKNASDVFIAADFPPAMKIDGKITPVAPQPLSAQHTKELVRAVMNDRQTEEFESKKEANFAI
NPPGLGRFRVSAYVQQGHAGMVLRKINTEIPTFDQLNLPLVLRDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSHNHD
HIITIEDPIEYVHQHKKSIITQREVGVDTDDWDIALKNTLRQAPDVILMGEIRDRETMGYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRSIISQRLVPIKSGRGRVAAVEILLNSPLVSDMIFKGEIAGLKEVMGRSRENG
MQTFDQSLFELYEADLISYEDALRNADSINDLRLKIKLYSEGSKSRDPLEGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=307242 DXT74_RS03945 WP_043639312.1 848941..850077(+) (pilU) [Chromobacterium sp. Rain0013]
ATGGAAAAAGACCAGGCTTCCAAGTTCATACTGGATCTGCTGAAGCACGCCACCGGCAAGAACGCCTCCGACGTTTTCAT
CGCCGCCGATTTCCCGCCGGCGATGAAGATAGACGGCAAGATCACTCCGGTGGCGCCGCAGCCTCTGTCCGCCCAGCACA
CCAAGGAACTGGTGCGCGCGGTGATGAACGACCGGCAGACCGAGGAGTTCGAATCCAAGAAGGAGGCGAACTTTGCGATC
AACCCGCCGGGTCTGGGGCGTTTCCGCGTTAGCGCCTATGTGCAGCAGGGCCATGCCGGCATGGTATTGCGCAAGATCAA
CACTGAAATCCCCACCTTCGACCAGCTCAACCTGCCGCTGGTGCTGCGCGACATCGCGCTGATCAAGCGCGGCCTGGTGA
TCTTCGTCGGCGGCACCGGTTCAGGCAAATCCACCTCGCTGGCCGCGCTGGTGGACTGGCGCAACAGCCATAACCACGAT
CACATCATCACCATCGAAGATCCGATCGAATACGTGCACCAGCACAAGAAGTCCATCATCACCCAGCGCGAGGTGGGCGT
GGACACCGATGATTGGGACATCGCCTTGAAGAACACCTTGCGCCAGGCGCCGGACGTGATTCTGATGGGCGAGATTCGCG
ATCGCGAAACCATGGGTTACGGGTTGCAGTTCGCCGAAACCGGCCATTTGTGCCTGGCCACGCTGCATGCCAACAACGCC
AACCAGGCGCTGGACCGCATTCTCAACTTCTTCCCCGAAGAGCGCCATCAACAGGTGTTGATGGATTTGTCGCTGAATAT
GCGCTCCATCATCTCTCAGCGGCTGGTGCCGATCAAAAGCGGCCGCGGCAGGGTGGCGGCGGTGGAAATTCTGCTCAACA
GCCCGCTGGTGTCCGACATGATCTTCAAGGGCGAAATCGCCGGCCTGAAGGAAGTGATGGGCCGCTCGCGCGAGAACGGC
ATGCAAACCTTCGATCAGTCCTTGTTCGAGTTGTACGAAGCGGATCTGATCAGCTACGAGGACGCCCTGCGCAACGCCGA
CTCCATTAACGACCTGAGGCTGAAGATCAAGCTTTACAGCGAGGGCTCCAAGTCGCGTGACCCGCTGGAAGGCATAGACC
ACCTCGACATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W0CSX0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.032

92.328

0.563
  pilU Acinetobacter baylyi ADP1

55.738

96.825

0.54
  pilU Vibrio cholerae strain A1552

53.602

91.799

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Acinetobacter baylyi ADP1

41.003

89.683

0.368
  pilT Pseudomonas aeruginosa PAK

40.653

89.153

0.362

Multiple sequence alignment