Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DVV14_RS09935 Genome accession   NZ_CP031472
Coordinates   2191527..2192564 (-) Length   345 a.a.
NCBI ID   WP_006958261.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain RE22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2186527..2197564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVV14_RS09905 (DVV14_09960) - 2186745..2187449 (+) 705 WP_064488074.1 endonuclease -
  DVV14_RS09910 (DVV14_09965) rsmE 2187558..2188289 (+) 732 WP_038509531.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DVV14_RS09915 (DVV14_09970) gshB 2188303..2189253 (+) 951 WP_019277263.1 glutathione synthase -
  DVV14_RS09920 (DVV14_09975) - 2189310..2189873 (+) 564 WP_019277262.1 YqgE/AlgH family protein -
  DVV14_RS09925 (DVV14_09980) ruvX 2189891..2190313 (+) 423 WP_064488075.1 Holliday junction resolvase RuvX -
  DVV14_RS09930 (DVV14_09985) pilU 2190409..2191515 (-) 1107 WP_064488076.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DVV14_RS09935 (DVV14_09990) pilT 2191527..2192564 (-) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  DVV14_RS09940 (DVV14_09995) - 2192589..2193305 (+) 717 WP_064488077.1 YggS family pyridoxal phosphate-dependent enzyme -
  DVV14_RS09945 (DVV14_10000) proC 2193333..2194151 (+) 819 WP_064488078.1 pyrroline-5-carboxylate reductase -
  DVV14_RS09950 (DVV14_10005) - 2194190..2194747 (+) 558 WP_006958264.1 YggT family protein -
  DVV14_RS09955 (DVV14_10010) yggU 2194747..2195037 (+) 291 WP_064488079.1 DUF167 family protein YggU -
  DVV14_RS09960 (DVV14_10015) - 2195114..2195851 (-) 738 WP_064488080.1 hypothetical protein -
  DVV14_RS09965 (DVV14_10020) - 2196070..2196414 (+) 345 WP_064488081.1 hypothetical protein -
  DVV14_RS09970 (DVV14_10025) - 2196392..2196853 (+) 462 WP_234425272.1 hypothetical protein -
  DVV14_RS26495 - 2197365..2197523 (-) 159 WP_019274018.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38121.49 Da        Isoelectric Point: 6.1894

>NTDB_id=307055 DVV14_RS09935 WP_006958261.1 2191527..2192564(-) (pilT) [Vibrio coralliilyticus strain RE22]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFNHSEVHRLVFEIMNDAQRSEFEERLEVDFSFALPDVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLEAPSIFENIANMEKGLILVTGPTGSGKSTTLAAMVDHINRHHNKHILTIED
PIEFVHTNNKCLINQREVHNDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVSKKIEVEQGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=307055 DVV14_RS09935 WP_006958261.1 2191527..2192564(-) (pilT) [Vibrio coralliilyticus strain RE22]
ATGGATATCGCTGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTATCTCCCAT
GGTACGCATTGATGGTGAAGTAAGAAAGCTTGGCGTACCAGCGTTCAACCATTCCGAAGTGCATCGTTTGGTGTTTGAAA
TTATGAACGATGCTCAGCGTAGCGAATTTGAAGAACGTCTTGAAGTCGACTTTTCTTTTGCACTTCCGGACGTAGGCCGT
TTTAGGGTCAACGCTTTTAACCAATCTCGTGGTTGTGCGGCCGTATTTCGTACCATTCCAACCGATATCCCGACTTTAGA
GCAGCTAGAAGCGCCATCGATCTTTGAAAACATCGCCAATATGGAGAAAGGATTAATCTTAGTCACTGGGCCAACGGGCT
CAGGTAAATCGACCACTTTGGCTGCAATGGTGGATCATATTAATCGTCACCACAATAAGCATATCCTGACTATTGAAGAT
CCGATTGAGTTTGTGCACACCAATAACAAATGTCTGATCAACCAGCGTGAAGTTCACAATGATACCCATAGTTTTAATAA
CGCGCTGCGCAGTGCATTGCGTGAAGATCCCGATGTCATTTTAGTCGGTGAGCTACGCGATCAGGAAACGATCAGTCTAG
CGCTGACCGCTGCTGAAACTGGCCACTTGGTATTTGGTACTTTGCACACCAGTTCGGCAGCGAAAACTATCGACCGTATC
ATTGATGTTTTCCCGGGTAGCGACAAAGGCATGGTGCGTTCCATGCTCTCTGAGTCTCTGCGTGCGGTGATTGCGCAGAA
ATTACTCAAGCGCATTGGCGGTGGGAGAACCGCTTGTCATGAAATCATGATGGCAACGCCTGCTATTCGTAATCTGATCC
GCGAAGACAAAGTCGCGCAAATGCAGTCCGTGATTCAGACTGGTGCGGCGCATGGTATGCAAACCATGGAGCAGCACTCC
AAACAGTTGATTGCTCAAGGTCTGGTTGATGCAGAAGAAGTGTCGAAAAAAATCGAAGTTGAGCAAGGTCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

100

0.872
  pilT Vibrio cholerae strain A1552

87.246

100

0.872
  pilT Acinetobacter baylyi ADP1

68.713

99.13

0.681
  pilT Acinetobacter baumannii strain A118

69.277

96.232

0.667
  pilT Acinetobacter baumannii D1279779

69.277

96.232

0.667
  pilT Acinetobacter nosocomialis M2

68.976

96.232

0.664
  pilT Pseudomonas stutzeri DSM 10701

66.766

97.681

0.652
  pilT Pseudomonas aeruginosa PAK

65.497

99.13

0.649
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

64.497

97.971

0.632
  pilT Neisseria gonorrhoeae MS11

63.235

98.551

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.652

0.496
  pilU Vibrio cholerae strain A1552

42.462

94.203

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

41.25

92.754

0.383

Multiple sequence alignment