Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DVV14_RS09930 Genome accession   NZ_CP031472
Coordinates   2190409..2191515 (-) Length   368 a.a.
NCBI ID   WP_064488076.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain RE22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2185409..2196515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVV14_RS09900 (DVV14_09955) - 2186168..2186665 (+) 498 WP_021458543.1 SprT family zinc-dependent metalloprotease -
  DVV14_RS09905 (DVV14_09960) - 2186745..2187449 (+) 705 WP_064488074.1 endonuclease -
  DVV14_RS09910 (DVV14_09965) rsmE 2187558..2188289 (+) 732 WP_038509531.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DVV14_RS09915 (DVV14_09970) gshB 2188303..2189253 (+) 951 WP_019277263.1 glutathione synthase -
  DVV14_RS09920 (DVV14_09975) - 2189310..2189873 (+) 564 WP_019277262.1 YqgE/AlgH family protein -
  DVV14_RS09925 (DVV14_09980) ruvX 2189891..2190313 (+) 423 WP_064488075.1 Holliday junction resolvase RuvX -
  DVV14_RS09930 (DVV14_09985) pilU 2190409..2191515 (-) 1107 WP_064488076.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DVV14_RS09935 (DVV14_09990) pilT 2191527..2192564 (-) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  DVV14_RS09940 (DVV14_09995) - 2192589..2193305 (+) 717 WP_064488077.1 YggS family pyridoxal phosphate-dependent enzyme -
  DVV14_RS09945 (DVV14_10000) proC 2193333..2194151 (+) 819 WP_064488078.1 pyrroline-5-carboxylate reductase -
  DVV14_RS09950 (DVV14_10005) - 2194190..2194747 (+) 558 WP_006958264.1 YggT family protein -
  DVV14_RS09955 (DVV14_10010) yggU 2194747..2195037 (+) 291 WP_064488079.1 DUF167 family protein YggU -
  DVV14_RS09960 (DVV14_10015) - 2195114..2195851 (-) 738 WP_064488080.1 hypothetical protein -
  DVV14_RS09965 (DVV14_10020) - 2196070..2196414 (+) 345 WP_064488081.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41230.85 Da        Isoelectric Point: 5.1932

>NTDB_id=307054 DVV14_RS09930 WP_064488076.1 2190409..2191515(-) (pilU) [Vibrio coralliilyticus strain RE22]
MDINAYLQGMLTEKASDLYITVGAPILYRVDGELRPRGESLSEAEVLTLLHSTMDEERQSDYSHSREANFAVVRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKDQFLFDLSMNLKGVIGQQLIRDKDGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMAKSQEIGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=307054 DVV14_RS09930 WP_064488076.1 2190409..2191515(-) (pilU) [Vibrio coralliilyticus strain RE22]
ATGGATATCAATGCTTATCTGCAAGGCATGCTGACTGAGAAAGCGTCAGACTTATACATCACTGTTGGGGCACCGATACT
TTATCGTGTTGATGGAGAGCTGAGACCGCGTGGAGAGAGCTTATCGGAAGCTGAAGTGCTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAGTGACTATAGCCACAGTCGAGAAGCGAACTTTGCCGTAGTGCGGGATTTTGGTCGCTTTCGT
GTTTCCGCTTTCTATCAGCGAGAATTACCCGGTGCGGTGATTCGTAGAATCGAAACCACCATACCCACATTTGATGACTT
AAAACTGCCCGAAACTCTGAAAGATCTTTCCATCGCCAAGCGCGGCTTAGTATTAGTAGTGGGCGCCACAGGTTCCGGTA
AATCCACAACAATGGCAGCGATGACAGGCTATCGAAATACTGAGCGCAGCGGCCATATTCTGACAGTTGAAGACCCGATT
GAGTTTGTCCACGAGCATCAGAAGTGCATCGTCACGCAACGTGAGGTTGGCTTAGACACAGAAAGTTATGAAGTCGCGCT
GAAGAACTCTTTGCGTCAGGCTCCTGATATGATCTTGATCGGTGAGATCCGCTCGCGAGAGACCATGGAGTACGCGATGA
CATTCGCTGAAACGGGGCATCTGTGTATGGCGACTTTGCACGCTAACAATGCTAACCAAGCTTTGGAAAGGATCCTTCAC
CTTGTACCGAAAGAGCAAAAAGACCAGTTCCTGTTTGATCTCTCCATGAACCTCAAAGGTGTTATCGGCCAGCAGTTGAT
CCGAGATAAAGACGGCCAAGGTCGTCATGGCGTGTTTGAAATTCTACTTAACAGTCCACGTGTATCGGATCTCATTCGAC
GCGGTGATCTGCATGAACTCAAATCAACCATGGCGAAATCTCAGGAAATTGGTATGCAGACGTTTGATCAGGCACTCTAC
CAATTGGTAGTGAATGACAAAATTACTGAAGAAGATGCGCTGCACAGTGCGGATTCAGCCAATGACCTGCGTATCATGCT
TAAAACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCTTTGGCTGATGTGAAGATTGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

92.12

0.408
  pilT Acinetobacter baylyi ADP1

42.522

92.663

0.394
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.154

88.315

0.372
  pilT Vibrio cholerae strain A1552

42.154

88.315

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

39.42

93.75

0.37
  pilT Neisseria gonorrhoeae MS11

39.42

93.75

0.37

Multiple sequence alignment