Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DWG18_RS03120 Genome accession   NZ_CP031413
Coordinates   642518..643555 (+) Length   345 a.a.
NCBI ID   WP_115645320.1    Uniprot ID   A0A345ZI06
Organism   Lysobacter sp. TY2-98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 637518..648555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWG18_RS03085 (DWG18_03085) - 637815..638651 (-) 837 WP_115645308.1 kinase -
  DWG18_RS03090 (DWG18_03090) pdxH 638669..639271 (+) 603 WP_115645310.1 pyridoxamine 5'-phosphate oxidase -
  DWG18_RS03095 (DWG18_03095) - 639280..640074 (+) 795 WP_115648005.1 ABC transporter substrate-binding protein -
  DWG18_RS03100 (DWG18_03100) - 640075..640536 (-) 462 WP_115645312.1 DUF4426 domain-containing protein -
  DWG18_RS03105 (DWG18_03105) proC 640546..641373 (-) 828 WP_115645314.1 pyrroline-5-carboxylate reductase -
  DWG18_RS03110 (DWG18_03110) - 641416..641703 (-) 288 WP_115645316.1 hypothetical protein -
  DWG18_RS03115 (DWG18_03115) - 641707..642552 (-) 846 WP_240318587.1 YggS family pyridoxal phosphate-dependent enzyme -
  DWG18_RS03120 (DWG18_03120) pilT 642518..643555 (+) 1038 WP_115645320.1 type IV pilus twitching motility protein PilT Machinery gene
  DWG18_RS03125 (DWG18_03125) pilU 643686..644861 (+) 1176 WP_115648006.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DWG18_RS03130 (DWG18_03130) - 644971..645540 (+) 570 WP_162823893.1 YqgE/AlgH family protein -
  DWG18_RS03135 (DWG18_03135) ruvX 645542..646006 (+) 465 WP_115645324.1 Holliday junction resolvase RuvX -
  DWG18_RS03140 (DWG18_03140) - 646010..646915 (+) 906 WP_115645326.1 aspartate carbamoyltransferase catalytic subunit -
  DWG18_RS03145 (DWG18_03145) - 646934..647968 (-) 1035 WP_162823681.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38520.37 Da        Isoelectric Point: 6.5056

>NTDB_id=306563 DWG18_RS03120 WP_115645320.1 642518..643555(+) (pilT) [Lysobacter sp. TY2-98]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAAAVFRTIPSEVLTLEDLNCPPIFRELIQQPQGLILVTGPTGSGKSTTLAAMLDFINKNEYAHLLSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVPSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQAVGMMTLDQHL
QDLVKRGLILKPQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=306563 DWG18_RS03120 WP_115645320.1 642518..643555(+) (pilT) [Lysobacter sp. TY2-98]
ATGGATATCGCCGAGCTTCTGGCGTTCTCGGTCAAGAACAAGGCGTCGGACCTTCATCTGTCGGCAGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTCCGTCGCATCAACATTCCGGCGCTCGAGCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGGGCCTGGCGCGC
TTCCGCGTGAACGCGTTCAACCAGAACCGCGGCGCGGCGGCGGTCTTCCGAACGATTCCGTCGGAAGTGCTGACGCTCGA
AGACCTGAACTGCCCGCCGATCTTCCGCGAGCTGATCCAGCAGCCGCAGGGCCTGATCCTCGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGCGATGCTCGACTTCATCAACAAGAACGAATATGCGCACCTGCTGTCGGTCGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
GGCCCTGCGTTCGGCGCTGCGTGAGGACCCGGACTACATCCTGGTCGGCGAGCTTCGCGACCTCGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACGTTGCACACGAGCTCGGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCTGGCGAAAAGCCGATGGTCCCTTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGTACGGCGGCCTGGGAAATCATGGTGGGCACACCCGCCATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGTCGATCCAGACGGGTCAGGCGGTCGGCATGATGACGCTCGACCAGCACCTG
CAGGATCTGGTCAAGCGCGGCCTGATCCTGAAGCCGCAGGCGCGCGAGTACGCCAAGGACAAGCGCCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345ZI06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.581

98.261

0.762
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.517

95.362

0.672
  pilT Vibrio cholerae strain A1552

70.517

95.362

0.672
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.826

100

0.478
  pilU Pseudomonas stutzeri DSM 10701

39.823

98.261

0.391
  pilU Acinetobacter baylyi ADP1

38.529

98.551

0.38
  pilU Vibrio cholerae strain A1552

40

94.203

0.377

Multiple sequence alignment