Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DWG18_RS03125 Genome accession   NZ_CP031413
Coordinates   643686..644861 (+) Length   391 a.a.
NCBI ID   WP_115648006.1    Uniprot ID   A0A345ZP55
Organism   Lysobacter sp. TY2-98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 638686..649861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWG18_RS03095 (DWG18_03095) - 639280..640074 (+) 795 WP_115648005.1 ABC transporter substrate-binding protein -
  DWG18_RS03100 (DWG18_03100) - 640075..640536 (-) 462 WP_115645312.1 DUF4426 domain-containing protein -
  DWG18_RS03105 (DWG18_03105) proC 640546..641373 (-) 828 WP_115645314.1 pyrroline-5-carboxylate reductase -
  DWG18_RS03110 (DWG18_03110) - 641416..641703 (-) 288 WP_115645316.1 hypothetical protein -
  DWG18_RS03115 (DWG18_03115) - 641707..642552 (-) 846 WP_240318587.1 YggS family pyridoxal phosphate-dependent enzyme -
  DWG18_RS03120 (DWG18_03120) pilT 642518..643555 (+) 1038 WP_115645320.1 type IV pilus twitching motility protein PilT Machinery gene
  DWG18_RS03125 (DWG18_03125) pilU 643686..644861 (+) 1176 WP_115648006.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DWG18_RS03130 (DWG18_03130) - 644971..645540 (+) 570 WP_162823893.1 YqgE/AlgH family protein -
  DWG18_RS03135 (DWG18_03135) ruvX 645542..646006 (+) 465 WP_115645324.1 Holliday junction resolvase RuvX -
  DWG18_RS03140 (DWG18_03140) - 646010..646915 (+) 906 WP_115645326.1 aspartate carbamoyltransferase catalytic subunit -
  DWG18_RS03145 (DWG18_03145) - 646934..647968 (-) 1035 WP_162823681.1 HDOD domain-containing protein -
  DWG18_RS03150 (DWG18_03150) mgtE 648043..649404 (-) 1362 WP_115645330.1 magnesium transporter -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 42969.49 Da        Isoelectric Point: 7.0158

>NTDB_id=306564 DWG18_RS03125 WP_115648006.1 643686..644861(+) (pilU) [Lysobacter sp. TY2-98]
MSPDTPLSPAAPAAPGGSIDFSSYLKLMAQHRASDLFITAGMPPSIKVHGKISPITQNPLTPQQARDLVLSVMSPAQREE
FEKTHECNFAIGVANVGRFRVSCFFQRNQVGMVLRRIESKIPTVEELALPPIIKTLAMTKRGIIMFVGATGTGKSTSLAA
MIGYRNKNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGH
LVLCTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKI
KEVMKESTNLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAAGLGDVGVADVR

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=306564 DWG18_RS03125 WP_115648006.1 643686..644861(+) (pilU) [Lysobacter sp. TY2-98]
ATGTCCCCCGACACCCCGCTGTCGCCCGCAGCACCTGCCGCGCCCGGCGGTTCGATCGACTTCTCGTCGTATCTGAAGCT
GATGGCGCAGCACCGCGCCTCGGACCTGTTCATCACGGCCGGCATGCCGCCGTCGATCAAGGTCCACGGCAAGATTTCGC
CGATCACCCAGAACCCGCTCACGCCGCAGCAGGCCCGTGACCTGGTGCTGTCGGTGATGAGCCCGGCGCAGCGCGAAGAG
TTCGAAAAGACGCATGAGTGCAACTTCGCGATCGGCGTGGCCAACGTCGGCCGCTTCCGCGTGAGCTGCTTCTTCCAGCG
TAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGAGCAAGATCCCGACGGTCGAGGAACTCGCGCTGCCGCCGATCATCA
AGACGCTCGCGATGACCAAGCGCGGCATCATCATGTTCGTCGGCGCGACCGGTACGGGTAAGTCGACGTCGCTCGCCGCG
ATGATCGGCTACCGCAACAAGAATTCGACCGGCCACATCATCACGATCGAGGATCCGATCGAATTCGTGCACAAGCACGA
GGGCTGCATCATCACGCAGCGCGAAGTCGGCATCGATACCGATTCGTGGGAAGCCGCGCTGAAGAACACGTTGCGCCAGG
CGCCCGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCGATCGCGTTCGCCGAAACCGGCCAC
CTCGTGCTCTGCACGCTGCATGCGAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGATCGCCG
CAACCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGTGGCGCAGCAGCTGATCCCGACGCCGGACGGCAAGG
GCCGTCGCGTCGCAATGGAAATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGATC
AAGGAAGTGATGAAGGAGTCGACCAACCTGGGCATGAAGACCTTCGACCAGGCATTGTTCGAGCTCTACCAGGCCGGCGA
GATCTCCTACGAGGACGCGCTGCGTTACGCCGACTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCG
ACGCCCGCACGCTGGCCGCGGGCCTCGGCGACGTGGGTGTGGCCGACGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345ZP55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.68

92.583

0.627
  pilU Acinetobacter baylyi ADP1

64.426

91.304

0.588
  pilU Vibrio cholerae strain A1552

53.933

91.049

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.077

93.606

0.394
  pilT Acinetobacter nosocomialis M2

40.988

87.98

0.361
  pilT Acinetobacter baumannii D1279779

40.988

87.98

0.361
  pilT Acinetobacter baumannii strain A118

40.988

87.98

0.361

Multiple sequence alignment