Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MG068_RS04660 Genome accession   NZ_CP031167
Coordinates   1000247..1001284 (+) Length   345 a.a.
NCBI ID   WP_006422551.1    Uniprot ID   -
Organism   Stenotrophomonas sp. ASS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 995247..1006284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG068_RS04635 (MG068_04620) soxR 996496..996930 (+) 435 WP_032127734.1 redox-sensitive transcriptional activator SoxR -
  MG068_RS04640 (MG068_04625) - 997004..997606 (-) 603 WP_132809397.1 short chain dehydrogenase -
  MG068_RS04645 (MG068_04630) - 997707..998582 (+) 876 WP_132809399.1 LysR family transcriptional regulator -
  MG068_RS04650 (MG068_04635) proC 998642..999463 (-) 822 WP_132809401.1 pyrroline-5-carboxylate reductase -
  MG068_RS04655 (MG068_04640) - 999482..1000159 (-) 678 WP_132809403.1 YggS family pyridoxal phosphate-dependent enzyme -
  MG068_RS04660 (MG068_04645) pilT 1000247..1001284 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  MG068_RS04665 (MG068_04650) pilU 1001364..1002527 (+) 1164 WP_049400675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MG068_RS04670 (MG068_04655) - 1002708..1003691 (+) 984 WP_107431433.1 aldo/keto reductase -
  MG068_RS04675 (MG068_04660) - 1003732..1004631 (-) 900 WP_132809405.1 LysR family transcriptional regulator -
  MG068_RS04680 (MG068_04665) - 1004935..1005147 (+) 213 WP_049462135.1 DUF4287 domain-containing protein -
  MG068_RS04685 (MG068_04670) - 1005244..1005693 (+) 450 WP_049421544.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.38 Da        Isoelectric Point: 6.6652

>NTDB_id=303829 MG068_RS04660 WP_006422551.1 1000247..1001284(+) (pilT) [Stenotrophomonas sp. ASS1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=303829 MG068_RS04660 WP_006422551.1 1000247..1001284(+) (pilT) [Stenotrophomonas sp. ASS1]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATCCCGTCGCTGGCGCGC
TTCCGTGTCAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGTTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAAAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCATGGCTTCAACGA
AGCGCTGCGCTCGGCACTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAACTGCGCGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACTCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGTTCGATGCTGTCCGAATCGCTGCGTGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCTGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGACTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment