Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MG068_RS04665 Genome accession   NZ_CP031167
Coordinates   1001364..1002527 (+) Length   387 a.a.
NCBI ID   WP_049400675.1    Uniprot ID   A0AA42HYU0
Organism   Stenotrophomonas sp. ASS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 996364..1007527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG068_RS04635 (MG068_04620) soxR 996496..996930 (+) 435 WP_032127734.1 redox-sensitive transcriptional activator SoxR -
  MG068_RS04640 (MG068_04625) - 997004..997606 (-) 603 WP_132809397.1 short chain dehydrogenase -
  MG068_RS04645 (MG068_04630) - 997707..998582 (+) 876 WP_132809399.1 LysR family transcriptional regulator -
  MG068_RS04650 (MG068_04635) proC 998642..999463 (-) 822 WP_132809401.1 pyrroline-5-carboxylate reductase -
  MG068_RS04655 (MG068_04640) - 999482..1000159 (-) 678 WP_132809403.1 YggS family pyridoxal phosphate-dependent enzyme -
  MG068_RS04660 (MG068_04645) pilT 1000247..1001284 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  MG068_RS04665 (MG068_04650) pilU 1001364..1002527 (+) 1164 WP_049400675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MG068_RS04670 (MG068_04655) - 1002708..1003691 (+) 984 WP_107431433.1 aldo/keto reductase -
  MG068_RS04675 (MG068_04660) - 1003732..1004631 (-) 900 WP_132809405.1 LysR family transcriptional regulator -
  MG068_RS04680 (MG068_04665) - 1004935..1005147 (+) 213 WP_049462135.1 DUF4287 domain-containing protein -
  MG068_RS04685 (MG068_04670) - 1005244..1005693 (+) 450 WP_049421544.1 GNAT family N-acetyltransferase -
  MG068_RS04690 (MG068_04675) - 1005787..1006494 (+) 708 WP_132809407.1 YitT family protein -
  MG068_RS04695 (MG068_04680) - 1006568..1007110 (-) 543 WP_107431676.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43172.60 Da        Isoelectric Point: 6.6615

>NTDB_id=303830 MG068_RS04665 WP_049400675.1 1001364..1002527(+) (pilU) [Stenotrophomonas sp. ASS1]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=303830 MG068_RS04665 WP_049400675.1 1001364..1002527(+) (pilU) [Stenotrophomonas sp. ASS1]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACCGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGA
CCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAGTGCAACTTCGCCATCGGCCTGTCCGGCGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGTTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAGGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACTCTGCGTCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTTGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGATCGTCGCAACCAGTTGCT
GATGGATCTGTCGCTGAACCTGAAGGGCGTGGTTGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGTTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGA
GGACGCGCTGCGCTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCCCGCACCC
TGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

94.832

0.643
  pilU Acinetobacter baylyi ADP1

63.585

92.248

0.587
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395

Multiple sequence alignment