Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DU509_RS07010 Genome accession   NZ_CP031115
Coordinates   1373956..1375071 (+) Length   371 a.a.
NCBI ID   WP_276129924.1    Uniprot ID   -
Organism   Rubrobacter indicoceani strain SCSIO 08198     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1368956..1380071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DU509_RS06995 ruvX 1371427..1371891 (+) 465 WP_119067893.1 Holliday junction resolvase RuvX -
  DU509_RS07000 mltG 1371888..1373072 (+) 1185 WP_119067895.1 endolytic transglycosylase MltG -
  DU509_RS07005 - 1373078..1373959 (+) 882 WP_119067897.1 shikimate dehydrogenase -
  DU509_RS07010 pilT 1373956..1375071 (+) 1116 WP_276129924.1 type IV pilus twitching motility protein PilT Machinery gene
  DU509_RS07015 - 1375193..1376413 (+) 1221 WP_119067900.1 type II secretion system F family protein -
  DU509_RS07020 - 1376489..1376779 (-) 291 WP_119067902.1 hypothetical protein -
  DU509_RS07025 - 1376779..1377462 (-) 684 WP_119067904.1 HNH endonuclease family protein -
  DU509_RS07030 rpe 1377633..1378334 (+) 702 WP_205544224.1 ribulose-phosphate 3-epimerase -
  DU509_RS07035 ribD 1378559..1379707 (+) 1149 WP_162924516.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40582.44 Da        Isoelectric Point: 6.9784

>NTDB_id=303462 DU509_RS07010 WP_276129924.1 1373956..1375071(+) (pilT) [Rubrobacter indicoceani strain SCSIO 08198]
MKPEPHLPELSEMLGGAVALEASDLHLTVGAPPSVRVSGRVRPLLSEGGAPCPPVSPSHARDVIYGILTDRQRKRLEEDL
ELDFSYTVANLSRFRVNVYVQRGSLGAAFRMIPHSITPLEDLGLPKVVADLADRPRGLVLVTGPTGSGKSTTLAAMIDRI
NEVREEHIMSVEDPIEFLHFHKKCLVNQREVGQDTRSFANALKHVLRQDPDVILVGEMRDLETIQLAITAAETGHMVFAT
LHTQDAPQTVDRVIDVFPPHQQQQVRTQLSNALEGVVTQTLVQRREERGGGRVVAAEVMVATSGVRNLIREGKNHQIYSA
MQTGAGVGMVTMDASLVDLVRRGEISKESALKKSSNPEEVHRMLGAGPRRG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=303462 DU509_RS07010 WP_276129924.1 1373956..1375071(+) (pilT) [Rubrobacter indicoceani strain SCSIO 08198]
TTGAAACCGGAGCCGCACCTCCCGGAGCTCTCCGAGATGCTCGGGGGAGCGGTTGCGCTCGAGGCGAGCGACCTGCACCT
GACGGTGGGGGCTCCGCCGTCGGTGAGGGTCTCCGGGCGGGTCCGGCCCCTGCTTTCTGAGGGCGGCGCCCCCTGCCCGC
CCGTCTCCCCCTCTCACGCAAGGGATGTGATCTACGGCATCCTCACCGACCGCCAGCGCAAGCGCCTGGAGGAGGACCTC
GAGCTTGACTTCTCCTACACCGTCGCAAACCTCTCGCGTTTTCGGGTGAACGTGTACGTGCAGAGGGGCTCTCTCGGGGC
GGCTTTTCGGATGATCCCGCACAGCATCACCCCCCTAGAGGACCTCGGCCTGCCGAAGGTGGTCGCAGACCTTGCAGACC
GCCCCCGGGGCCTTGTCCTTGTCACGGGCCCTACGGGGAGCGGCAAGTCAACGACCCTCGCTGCGATGATAGACCGCATA
AACGAGGTGCGTGAAGAGCACATAATGAGCGTGGAGGACCCGATAGAGTTTCTTCACTTCCACAAGAAGTGCCTTGTAAA
CCAGCGCGAGGTGGGCCAGGATACCCGCTCCTTTGCAAACGCCCTGAAGCACGTCCTGAGGCAGGACCCGGACGTGATCC
TTGTAGGCGAGATGCGAGACCTCGAGACCATACAGCTTGCCATCACCGCCGCCGAGACCGGGCACATGGTCTTTGCAACG
CTTCACACCCAGGACGCCCCGCAGACGGTAGACCGGGTGATAGACGTCTTTCCGCCCCACCAGCAGCAGCAGGTAAGGAC
GCAGCTCTCAAACGCTCTTGAGGGGGTAGTAACGCAGACGCTGGTGCAAAGAAGAGAGGAGAGAGGCGGCGGGCGGGTGG
TAGCGGCGGAGGTGATGGTCGCAACAAGCGGGGTAAGGAACCTTATAAGGGAGGGGAAGAACCACCAGATCTACTCCGCG
ATGCAGACCGGAGCCGGGGTGGGGATGGTGACGATGGACGCGAGCCTGGTGGACCTTGTGAGGCGGGGTGAGATCTCAAA
GGAGAGCGCCCTGAAGAAGTCCTCGAACCCCGAAGAGGTGCACAGGATGCTCGGTGCCGGGCCCAGGAGGGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

54.857

94.34

0.518
  pilT Acinetobacter nosocomialis M2

54.286

94.34

0.512
  pilT Acinetobacter baumannii D1279779

54.286

94.34

0.512
  pilT Acinetobacter baumannii strain A118

54.286

94.34

0.512
  pilT Legionella pneumophila strain Lp02

53.714

94.34

0.507
  pilT Legionella pneumophila strain ERS1305867

53.714

94.34

0.507
  pilT Pseudomonas aeruginosa PAK

53.143

94.34

0.501
  pilT Pseudomonas stutzeri DSM 10701

52.571

94.34

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.986

95.687

0.488
  pilT Neisseria meningitidis 8013

49.858

95.148

0.474
  pilT Vibrio cholerae strain A1552

50.725

92.992

0.472
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.725

92.992

0.472
  pilT Neisseria gonorrhoeae MS11

49.432

94.879

0.469
  pilU Pseudomonas stutzeri DSM 10701

42.061

96.765

0.407
  pilU Vibrio cholerae strain A1552

41.573

95.957

0.399
  pilU Acinetobacter baylyi ADP1

37.96

95.148

0.361

Multiple sequence alignment