Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DUN60_RS12065 Genome accession   NZ_CP031055
Coordinates   2749099..2750136 (-) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio splendidus strain Vibrio sp     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2744099..2755136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DUN60_RS12040 rsmE 2744794..2745525 (+) 732 WP_065205683.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DUN60_RS12045 gshB 2745535..2746485 (+) 951 WP_004735482.1 glutathione synthase -
  DUN60_RS12050 - 2746602..2747165 (+) 564 WP_114634023.1 YqgE/AlgH family protein -
  DUN60_RS12055 ruvX 2747221..2747643 (+) 423 WP_017075420.1 Holliday junction resolvase RuvX -
  DUN60_RS12060 pilU 2747975..2749084 (-) 1110 WP_114634024.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DUN60_RS12065 pilT 2749099..2750136 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  DUN60_RS12070 - 2750163..2750867 (+) 705 WP_114634025.1 YggS family pyridoxal phosphate-dependent enzyme -
  DUN60_RS12075 proC 2750952..2751770 (+) 819 WP_114634026.1 pyrroline-5-carboxylate reductase -
  DUN60_RS12080 - 2751846..2752403 (+) 558 WP_004735475.1 YggT family protein -
  DUN60_RS12085 yggU 2752433..2752723 (+) 291 WP_050621014.1 DUF167 family protein YggU -
  DUN60_RS12090 - 2752752..2753183 (+) 432 WP_004735472.1 DUF4426 domain-containing protein -
  DUN60_RS12095 - 2753232..2753831 (+) 600 WP_114634027.1 XTP/dITP diphosphatase -
  DUN60_RS12100 hemW 2753831..2755018 (+) 1188 WP_114634028.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=302972 DUN60_RS12065 WP_004735478.1 2749099..2750136(-) (pilT) [Vibrio splendidus strain Vibrio sp]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=302972 DUN60_RS12065 WP_004735478.1 2749099..2750136(-) (pilT) [Vibrio splendidus strain Vibrio sp]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGCCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAATTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTGTTTCGTACCATTCCAGTAGAGATTCCCACCCTTGA
ACAGCTAGGGGCTCCTGAGATATTTGAAAGAATATCTAATTACGAAAAAGGTCTAGTATTGGTCACTGGGCCCACTGGTT
CAGGTAAGTCGACAACTTTGGCTGCGATGGTGGACTATGTTAACCGTAACCATAATAAGCATATATTGACGATTGAAGAT
CCGATCGAATTTGTTCATACCAACAACAAATGCCTAATCAATCAACGAGAAGTGCACCGCGACACTCACAGCTTCAAAGC
GGCGTTGCGTAGTGCGTTACGTGAAGATCCCGACGTAATCCTTGTGGGTGAACTTCGTGACCAAGAGACGATTAGCTTAG
CGCTAACCGCAGCAGAAACGGGTCACTTAGTTTTTGGTACTTTGCACACGAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGGAGCGACAAAGACATGGTGCGTTCAATGCTATCTGAATCGTTACGTTCGGTGATTGCCCAAAA
GCTGTTAAAGCGTGTCGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATCAGAAACTTGATCC
GTGAAGATAAGGTCGCGCAGATGTATTCGATTATTCAAACGGGCGCGGCACATGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGTTGATGGCTCAAGGCCTGGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment